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AB122157

Anti-RSPH4A antibody

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(1 Publication)

Rabbit Polyclonal RSPH4A antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RSPH4A aa 1-100.

View Alternative Names

RSHL3, RSPH4A, Radial spoke head protein 4 homolog A, Radial spoke head-like protein 3

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH4A antibody (AB122157)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH4A antibody (AB122157)

ab122157, at 1/75, staining RSPH4A in paraffin embedded Human bronchus tissue by Immunohistochemistry.

Western blot - Anti-RSPH4A antibody (AB122157)
  • WB

Unknown

Western blot - Anti-RSPH4A antibody (AB122157)

All lanes:

Western blot - Anti-RSPH4A antibody (ab122157) at 1/250 dilution

Lane 1:

RT-4 cell lysate

Lane 2:

U-251 MG cell lysate

Lane 3:

Human plasma

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 81 kDa

true

Immunocytochemistry/ Immunofluorescence - Anti-RSPH4A antibody (AB122157)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RSPH4A antibody (AB122157)

ab122157, at 4 µg/ml, staining RSPH4A (green) in PFA-fixed, Triton-permeabilized Human A-431 cells by Immunofluorescence.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RSPH4A aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q5TD94

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/500", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSPH4A also known as radial spoke head 4 homolog A is a protein with a mass of approximately 77 kDa. It plays a mechanical role in the structure and function of cilia. RSPH4A is expressed mainly in tissues where cilia are present such as respiratory epithelium and sperm cells. The protein is an essential component of the radial spokes which are structural proteins in the axoneme of motile cilia and flagella.
Biological function summary

The proper assembly and function of cilia are contingent upon the function of RSPH4A. As part of the radial spoke complex RSPH4A interacts with other radial spoke components contributing to the regulation of ciliary and flagellar motility. This regulation is necessary for effective fluid movement across cell surfaces and for ensuring the sperm's ability to navigate toward the ovum highlighting its role in fertility.

Pathways

The integrity of cilia movement requires functional radial spoke proteins and related pathways. RSPH4A participates in the ciliary motility pathway influencing components like dynein arms and other spoke proteins such as RSPH1. These proteins collectively modulate the interaction of dynein arms with microtubules necessary for the effective beating of cilia and flagella affecting processes such as respiratory airflow and reproductive capabilities.

RSPH4A mutations have a critical link to primary ciliary dyskinesia (PCD) a condition characterized by chronic respiratory tract infections and fertility issues. RSPH4A dysfunction leads to abnormal cilia movement impacting respiratory clearance and sperm motility. Patients with PCD often exhibit disrupted coordination of cilia which can co-occur with related disorders facilitated by pathogenic variations in proteins like DNAH5 another axonemal protein.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the axonemal radial spoke head which plays an important role in ciliary motility (PubMed : 19200523). Essential for triplet radial spokes (RS1, RS2 and RS3) head assembly in the motile cilia (By similarity).
See full target information RSPH4A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 16:58-67 PubMed33364434

2020

SLC12A8 plays a key role in bladder cancer progression and EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Lai Li,Zheng-Feng Li,Qing-Wei Cao,Wen-Zhen Wang
View all publications
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Product promise

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