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AB223045

Anti-RSPH9 antibody

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(1 Publication)

Rabbit Polyclonal RSPH9 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RSPH9 aa 50-200.

View Alternative Names

C6orf206, MRPS18AL1, RSPH9, Radial spoke head protein 9 homolog

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH9 antibody (AB223045)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH9 antibody (AB223045)

Paraffin-embedded human melanoma cancer tissue stained for RSPH9 using ab223045 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH9 antibody (AB223045)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPH9 antibody (AB223045)

Paraffin-embedded human testis tissue stained for RSPH9 using ab223045 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-RSPH9 antibody (AB223045)
  • WB

Supplier Data

Western blot - Anti-RSPH9 antibody (AB223045)

All lanes:

Western blot - Anti-RSPH9 antibody (ab223045) at 1/2000 dilution

Lane 1:

Mouse liver lysate

Lane 2:

Mouse skeletal muscle lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 27 kDa,31 kDa,37 kDa

Observed band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RSPH9 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9H1X1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSPH9 also known as Radial Spoke Head 9 Homolog is a protein with an approximate mass of 29 kDa. It plays a mechanical role in the structural integrity and function of cilia and flagella acting as an important element of the radial spoke complex. Expression of RSPH9 is found mainly in tissues with motile cilia such as the respiratory epithelium brain ventricles and the reproductive system. The protein stabilizes this complex facilitating proper movement of cilia and flagella.
Biological function summary

The radial spoke complex containing RSPH9 works together to regulate the bending of axonemes which is essential for ciliary and flagellar motility. RSPH9 assists with converting chemical energy into motion enabling effective fluid movement across surfaces or propulsion of cells. It forms part of a larger assembly that includes other radial spoke and axonemal dynein proteins each contributing to coordinated movement necessary for normal physiological functions.

Pathways

Effective ciliary function relies on the coordination of RSPH9 with proteins involved in the dynein-arm activation and intraflagellar transport. RSPH9 associates with the dynein regulatory complex pathway that directly affects ciliary beat frequency. Interaction with proteins such as dynein heavy chain (DNAH5) and intraflagellar transport complex facilitates the intricate process of axonemal microtubule arrangement and transportation required for normal cellular function.

Defects in RSPH9 are linked to conditions like Primary Ciliary Dyskinesia (PCD) and certain types of male infertility due to impaired motility of sperm flagella. These disorders arise due to mutations that disrupt the normal structure and function of cilia and flagella. Proteins such as radial spoke head protein 4 homolog (RSPH4A) also contribute to these conditions with both RSPH9 and RSPH4A frequently identified in genetic studies of PCD highlighting their critical roles in maintaining ciliary function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia (PubMed : 19200523). Essential for both the radial spoke head assembly and the central pair microtubule stability in ependymal motile cilia (By similarity). Required for motility of olfactory and neural cilia and for the structural integrity of ciliary axonemes in both 9+0 and 9+2 motile cilia (By similarity).
See full target information RSPH9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 75:205-213 PubMed38736267

2024

Campothecin suppresses cell proliferation and migration in head and neck squamous cell carcinoma by blocking RAB27A-mediated phosphatidylinositol 3 kinase (PI3K)/protein kinase B (AKT) pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Y Zhao,Y Wang,L Zhao,L Qu,J H Zheng
View all publications
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Product promise

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