JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB233113

Anti-RSPO3 antibody

Be the first to review this product! Submit a review

|

(4 Publications)

Rabbit Polyclonal RSPO3 antibody. Suitable for WB, IHC-P and reacts with Pig, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human RSPO3.

View Alternative Names

PWTSR, THSD2, RSPO3, R-spondin-3, Protein with TSP type-1 repeat, Roof plate-specific spondin-3, Thrombospondin type-1 domain-containing protein 2, hPWTSR, hRspo3

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)

Paraffin-embedded human breast cancer tissue stained for RSPO3 using ab233113 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)

Paraffin-embedded human pancreatic cancer tissue stained for RSPO3 using ab233113 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)

Paraffin-embedded human glioma tissue stained for RSPO3 using ab233113 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)

Paraffin-embedded human bile duct cancer tissue stained for RSPO3 using ab233113 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO3 antibody (AB233113)

Paraffin-embedded human liver tissue stained for RSPO3 using ab233113 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-RSPO3 antibody (AB233113)
  • WB

Supplier Data

Western blot - Anti-RSPO3 antibody (AB233113)

All lanes:

Western blot - Anti-RSPO3 antibody (ab233113) at 2 µg/mL

All lanes:

Recombinant human RSPO3 protein

Predicted band size: 31 kDa

false

Western blot - Anti-RSPO3 antibody (AB233113)
  • WB

Supplier Data

Western blot - Anti-RSPO3 antibody (AB233113)

All lanes:

Western blot - Anti-RSPO3 antibody (ab233113) at 2 µg/mL

All lanes:

Pig small intestine lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Pig

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human RSPO3.

Q9BXY4

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab233113 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

R-spondin 3 (RSPO3) also known as CRISTIN3 is a protein with a molecular weight of approximately 27 kDa. Mechanically RSPO3 acts as a regulatory protein involved in modulating the Wnt signaling pathway by facilitating the interaction between LGR receptors and Frizzled LRP6 co-receptors. RSPO3 is expressed in various tissues with high levels observed in the placenta and also in the lung and kidney. This distribution suggests RSPO3 has diverse roles in different physiological contexts.
Biological function summary

RSPO3 functions as a growth factor-like protein that can enhance Wnt signaling leading to the stabilization of β-catenin and promoting cell proliferation and differentiation. RSPO3 is part of a larger protein complex that includes LGR4/5/6 receptors and the Wnt signaling ligands. This interaction is essential in processes like embryonic development and tissue regeneration illustrating its supportive role in maintaining cellular dynamics.

Pathways

RSPO3 plays a significant role in the Wnt/β-catenin signaling pathway where it works closely with proteins such as LRP6 and Frizzled receptors. It also connects with the non-canonical Wnt signaling pathway. This interaction enhances signal transduction vital for cell fate determination. RSPO3’s ability to bind LGR4 and facilitate its role in pathways related to growth and development emphasizes its importance in cellular communication and regulation.

Aberrant expression or mutations of RSPO3 have associations with cancer particularly colorectal cancer through alterations in the Wnt/β-catenin pathway. RSPO3 supports oncogenic processes by enhancing β-catenin stability leading to uncontrolled cell proliferation. It is also relevant in vascular diseases where its interaction with other proteins like VEGFA can influence angiogenesis. Understanding RSPO3’s function in these pathways aids in developing targeted therapies for these conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway (PubMed : 21727895, PubMed : 21909076, PubMed : 22615920). Acts as a key regulator of angiogenesis by controlling vascular stability and pruning : acts by activating the non-canonical Wnt signaling pathway in endothelial cells (By similarity) (PubMed : 21727895, PubMed : 21909076, PubMed : 22615920). Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3 (PubMed : 29769720).
See full target information RSPO3
style
left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed37998393

2023

LGR5 Expression Predicting Poor Prognosis Is Negatively Correlated with WNT5A in Colon Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lubna M Mehdawi,Souvik Ghatak,Payel Chakraborty,Anita Sjölander,Tommy Andersson

Acta biochimica Polonica 70:295-304 PubMed37220402

2023

Circular RNA-AnnexinA7 accelerates cisplatin resistance in non-small cell lung cancer via modulating microRNA-545-3p to mediate Cyclin D1.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yao,Hai Yang Zhang,Shuang Gu,Jin Long Zou,Qiang Zhang,Ri Chu Qu

Stem cell research & therapy 12:330 PubMed34099046

2021

Rspo3 regulates the abnormal differentiation of small intestinal epithelial cells in diabetic state.

Applications

Unspecified application

Species

Unspecified reactive species

Ti-Dong Shan,Han Yue,Xue-Guo Sun,Yue-Ping Jiang,Li Chen

Annals of translational medicine 8:1351 PubMed33313096

2020

RSPO3 is a marker candidate for predicting tumor aggressiveness in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Haifeng Gu,Hua Tu,Lili Liu,Ting Liu,Zhimin Liu,Wei Zhang,Jihong Liu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB95363

Anti-Glypican 3 antibody [SP86]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Glypican 3 antibody [SP86] (AB95363)

  • 0
  • 0
Primary Antibodies

AB75851

Anti-Cullin 3/CUL-3 antibody [EPR3196Y]

RabMAb|Recombinant

Western blot - Anti-Cullin 3/CUL-3 antibody [EPR3196Y] (AB75851)

  • 5
  • 2
Primary Antibodies

AB106556

Anti-RSPO1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSPO1 antibody (AB106556)

  • 3
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com