AB189515

Anti-RSPO4 antibody [EPR9806(2)]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal RSPO4 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications.

View Alternative Names

C20orf182, RSPO4, R-spondin-4, Roof plate-specific spondin-4, hRspo4

2 Images

Supplier Data

Western blot - Anti-RSPO4 antibody [EPR9806(2)] (AB189515)

All lanes:

Western blot - Anti-RSPO4 antibody [EPR9806(2)] (ab189515) at 1/5000 dilution

Lane 1:

Mouse brain at 10 µg

Lane 2:

Rat brain at 10 µg

Lane 3:

C6 at 10 µg

Lane 4:

Raw 264.7 cell lysate at 10 µg

Lane 5:

NIH3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Predicted band size: 26 kDa

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Supplier Data

Western blot - Anti-RSPO4 antibody [EPR9806(2)] (AB189515)

All lanes:

Western blot - Anti-RSPO4 antibody [EPR9806(2)] (ab189515) at 1/20000 dilution

All lanes:

Human RSPO4 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9806(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RSPO4 also known as R-spondin 4 is a protein that plays a role in cell signaling and regulates growth and development processes. Its molecular weight is approximately 22 kDa. RSPO4 is active in various tissues with expression in the intestine bone and other epithelial tissues. It functions by enhancing Wnt signaling an important mechanism for cell proliferation and differentiation through binding to LGR receptors which then interact with ZNRF3 and RNF43 to prevent the degradation of Wnt co-receptors.

Biological function summary

The role of RSPO4 involves the modulation of cellular communication essential for tissue development and regeneration. It does not work alone; instead it is part of a complex that includes LGR4 LGR5 and LGR6 receptors which facilitates the amplification of Wnt signals. This activity supports processes such as stem cell maintenance and epithelial tissue homeostasis.

Pathways

RSPO4 functions as an important enhancer of the Wnt/β-catenin signaling pathway and also interacts with non-canonical Wnt pathways. RSPO4's activity connects with proteins such as β-catenin that function downstream in these pathways ensuring proper signaling for cell fate determination and tissue morphogenesis.

Disruption in RSPO4 expression relates to developmental issues and certain types of cancers. RSPO4 mutations are known to cause anonychia congenita a condition characterized by the absence of nails. Alterations in RSPO4 signaling have also been linked to colorectal cancer with abnormal Wnt signaling being a common factor in these pathologies. The interactions between RSPO4 and LGR receptors serve as potential therapeutic targets in these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors (PubMed : 29769720). Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway (PubMed : 21727895, PubMed : 21909076).

See full target information RSPO4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nutrients 17: PubMed40573160

2025

High Dietary Phosphorus Impairs Bone Microarchitecture and Induces Alterations in the LGR4-R-Spondins Axis in Rats with Normal Renal Function.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Fernández-Villabrille,Francisco Baena-Huerta,Laura Suárez-Fernández,Elena Nefyodova,Paula Calvó,Nerea González-García,Helena Gil-Peña,Carlos Gómez-Alonso,Cristina Alonso-Montes,Manuel Naves-Díaz,Christa Maes,Natalia Carrillo-López,Sara Panizo

The journal of gene medicine 23:e3382 PubMed34350661

2021

Inhibition of the long non-coding RNA UNC5B-AS1/miR-4455/RSPO4 axis reduces cervical cancer growth in vitro and in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Fu,Yuanyuan Zhang,Min Wang,Junwu Hu,Yuelan Fang

Stem cell research & therapy 12:330 PubMed34099046

2021

Rspo3 regulates the abnormal differentiation of small intestinal epithelial cells in diabetic state.

Applications

Unspecified application

Species

Unspecified reactive species

Ti-Dong Shan,Han Yue,Xue-Guo Sun,Yue-Ping Jiang,Li Chen

Medical oncology (Northwood, London, England) 37:90 PubMed32926243

2020

Associated analysis of PER1/TUBB2B with endometrial cancer development caused by circadian rhythm disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoxia Wang,Hui Wang,Zhaojun Wang,Simin He,Zhiping Jiang,Changping Yan,Sanyuan Zhang,Tong Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RSPO4 antibody [EPR9806(2)]

Western blot - Anti-RSPO4 antibody [EPR9806(2)] (AB189515)

Western blot - Anti-RSPO4 antibody [EPR9806(2)] (AB189515)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise