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AB198791

Anti-RST antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal RST antibody. C-terminal. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SLC22A12 aa 500-550.

View Alternative Names

OATL4, URAT1, UNQ6453/PRO34004, SLC22A12, Solute carrier family 22 member 12, Organic anion transporter 4-like protein, Renal-specific transporter, Urate anion exchanger 1, Urate:anion antiporter SLC22A12, RST

1 Images
Western blot - Anti-RST antibody - C-terminal (AB198791)
  • WB

Supplier Data

Western blot - Anti-RST antibody - C-terminal (AB198791)

8% SDS PAGE

All lanes:

Western blot - Anti-RST antibody - C-terminal (ab198791) at 1/200 dilution

Lane 1:

Hela cell lysate at 40 µg

Lane 2:

293T cell lysate at 40 µg

Predicted band size: 48 kDa,60 kDa

false

Exposure time: 40s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLC22A12 aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q96S37

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RST protein also known as RSTP1 has a significant role in cellular mechanics. It is a protein with a molecular mass of approximately 56 kDa and shows expression in multiple tissue types especially in the liver and heart. Functionally RST acts in the regulation of ion transport across cellular membranes. Its structure allows it to interact with both lipids and proteins facilitating effective shuttling of specific ions most notably calcium ions that are essential for maintaining proper cellular function.
Biological function summary

The mechanism of RST significantly contributes to the homeostasis of cellular ion concentrations. It forms part of the larger calcium ion regulation complex interacting closely with proteins like calmodulin. This interaction enables RST to play a vital part in maintaining calcium levels which is important for cellular signaling and muscle contraction processes. As part of the complex RST assists in the rapid response required during fluctuations in cellular ionic balance.

Pathways

Calcium ion regulation stands central where RST exerts its influence. Notably it participates in the signal transduction pathway which involves calcium as a second messenger to facilitate cellular responses to external stimuli. Additionally RST is involved in metabolic pathways that hinge on calcium concentration regulation working alongside related proteins such as phospholipase C which also has a role in signal transduction processes. These pathways are integral to maintaining cellular function and response to changes in the environment.

RST holds relevance in conditions related to calcium imbalance including cardiac arrhythmias and type 2 diabetes. In cardiac arrhythmias the dysregulation of RST can impact calcium channels influencing heart rhythm stability. Interactions with other proteins such as troponin compound these effects by further altering calcium sensitivity in cardiac tissues. In type 2 diabetes alterations in calcium signaling associated with RST maladaptations contribute to the insulin secretion process. These examples highlight RST’s varied influence on disease processes linked to signficant global health challenges.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Electroneutral antiporter that translocates urate across the apical membrane of proximal tubular cells in exchange for monovalent organic or inorganic anions (PubMed : 12024214, PubMed : 22194875, PubMed : 35144162, PubMed : 35462902). Involved in renal reabsorption of urate and helps maintaining blood levels of uric acid (PubMed : 12024214, PubMed : 22194875). Mediates urate uptake by an exchange with organic anions such as (S)-lactate and nicotinate, and inorganic anion Cl(-) (PubMed : 12024214). Other inorganic anions such as Br(-), I(-) and NO3(-) may also act as counteranions that exchange for urate (PubMed : 12024214). Also mediates orotate tubular uptake coupled with nicotinate efflux and to a lesser extent with lactate efflux, therefore displaying a potential role in orotate renal reabsorption (PubMed : 21350910). Orotate transport is Cl(-)-dependent (PubMed : 21350910).
See full target information SLC22A12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 10: PubMed33182520

2020

Comparison of the Effects of Nonprotein and Protein Nitrogen on Apoptosis and Autophagy of Rumen Epithelial Cells in Goats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiwei Kong,Chuanshe Zhou,Jinhe Kang,Zhiliang Tan
View all publications
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