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AB254600

Anti-RSU1 antibody

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(1 Publication)

Rabbit Polyclonal RSU1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RSU1 aa 100 to C-terminus.

View Alternative Names

RSP1, RSU1, Ras suppressor protein 1, RSP-1, Rsu-1

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSU1 antibody (AB254600)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RSU1 antibody (AB254600)

Paraffin-embedded human liver tissue stained for RSU1 using ab254600 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-RSU1 antibody (AB254600)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RSU1 antibody (AB254600)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling RSU1 using ab254600 at 4µg/ml (green) in ICC/IF.

Western blot - Anti-RSU1 antibody (AB254600)
  • WB

Supplier Data

Western blot - Anti-RSU1 antibody (AB254600)

All lanes:

Western blot - Anti-RSU1 antibody (ab254600) at 0.4 µg/mL

All lanes:

RT4 (human urinary bladder cancer cell line) cell lysate

Predicted band size: 32 kDa

false

Western blot - Anti-RSU1 antibody (AB254600)
  • WB

Supplier Data

Western blot - Anti-RSU1 antibody (AB254600)

All lanes:

Western blot - Anti-RSU1 antibody (ab254600) at 0.4 µg/mL

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (rat Wistar bladder tumor cell line) cell lysate

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human RSU1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15404

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RSU1 protein also referred to as Ras suppressor protein 1 is an influential regulator of cell adhesion and migration. It has a molecular mass of approximately 33 kDa. RSU1 mainly expresses in the brain and other tissues where it participates in various signaling pathways. The protein interfaces with integrin-linked kinase (ILK) and modulates actin cytoskeleton organization impacting cellular motility and interactions with the extracellular environment.
Biological function summary

The RSU1 protein integrates into a complex alongside ILK and other partners to affect cytoskeletal dynamics. It impacts cellular processes such as spreading adhesion and migration by modulating focal adhesion structures. RSU1 balances signaling cascades that influence cellular tension and stability contributing to cellular responses to external signals. This involvement makes RSU1 a notable player in maintaining cellular architecture and function.

Pathways

Involvement of RSU1 in the integrin and Rac1 signaling pathways highlights its functional roles. Through its interaction with ILK and p21-activated kinase (PAK) RSU1 regulates important steps in these pathways. The integration of RSU1 into these pathways underlines its contribution to cell shape modulation and movement emphasizing its role in normal cellular processes and homeostasis.

RSU1 research connects it with cancer progression and neurodegenerative diseases. Its dysregulation associates with altered cell migration and adhesion which are critical in cancer metastasis. RSU1 also engages with proteins like ILK which are implicated in tumorigenic pathways. In neurodegenerative contexts altered RSU1 expression patterns might affect neuronal structure and function linking it to conditions like Alzheimer's disease where cellular adhesion and cytoskeletal integrity are compromised.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Potentially plays a role in the Ras signal transduction pathway. Capable of suppressing v-Ras transformation in vitro.
See full target information RSU1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 6: PubMed37604583

2023

Tumor necrosis factor mediates USE1-independent FAT10ylation under inflammatory conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Leonie Schnell,Alina Zubrod,Nicola Catone,Johanna Bialas,Annette Aichem
View all publications
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Product promise

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