AB191409

Anti-RSU1 antibody [EPR16127]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RSU1 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

RSP1, RSU1, Ras suppressor protein 1, RSP-1, Rsu-1

4 Images

Supplier Data

Immunoprecipitation - Anti-RSU1 antibody [EPR16127] (AB191409)

Immunoprecipitation analysis of Caco 2 cell lysate labeling RSU1 using ab191409 at 1/100 dilution (Lane 1). An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of Caco 2 cell lysate.

All lanes:

Immunoprecipitation - Anti-RSU1 antibody [EPR16127] (ab191409)

Predicted band size: 32 kDa

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Supplier Data

Western blot - Anti-RSU1 antibody [EPR16127] (AB191409)

All lanes:

Western blot - Anti-RSU1 antibody [EPR16127] (ab191409) at 1/10000 dilution

All lanes:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-RSU1 antibody [EPR16127] (AB191409)

All lanes:

Western blot - Anti-RSU1 antibody [EPR16127] (ab191409) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Lane 9:

C6 cell lysate at 10 µg

Lane 10:

Raw264.7 cell lysate at 10 µg

Lane 11:

PC12 cell lysate at 10 µg

Lane 12:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

Supplier Data

Western blot - Anti-RSU1 antibody [EPR16127] (AB191409)

All lanes:

Western blot - Anti-RSU1 antibody [EPR16127] (ab191409) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Caco 2 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

Carrier free

Anti-RSU1 antibody [EPR16127] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16127

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The RSU1 protein also referred to as Ras suppressor protein 1 is an influential regulator of cell adhesion and migration. It has a molecular mass of approximately 33 kDa. RSU1 mainly expresses in the brain and other tissues where it participates in various signaling pathways. The protein interfaces with integrin-linked kinase (ILK) and modulates actin cytoskeleton organization impacting cellular motility and interactions with the extracellular environment.

Biological function summary

The RSU1 protein integrates into a complex alongside ILK and other partners to affect cytoskeletal dynamics. It impacts cellular processes such as spreading adhesion and migration by modulating focal adhesion structures. RSU1 balances signaling cascades that influence cellular tension and stability contributing to cellular responses to external signals. This involvement makes RSU1 a notable player in maintaining cellular architecture and function.

Pathways

Involvement of RSU1 in the integrin and Rac1 signaling pathways highlights its functional roles. Through its interaction with ILK and p21-activated kinase (PAK) RSU1 regulates important steps in these pathways. The integration of RSU1 into these pathways underlines its contribution to cell shape modulation and movement emphasizing its role in normal cellular processes and homeostasis.

RSU1 research connects it with cancer progression and neurodegenerative diseases. Its dysregulation associates with altered cell migration and adhesion which are critical in cancer metastasis. RSU1 also engages with proteins like ILK which are implicated in tumorigenic pathways. In neurodegenerative contexts altered RSU1 expression patterns might affect neuronal structure and function linking it to conditions like Alzheimer's disease where cellular adhesion and cytoskeletal integrity are compromised.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Potentially plays a role in the Ras signal transduction pathway. Capable of suppressing v-Ras transformation in vitro.

See full target information RSU1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancers 17: PubMed40075680

2025

Cystatin M/E Ameliorates Multiple Myeloma-Induced Hyper Osteolytic Bone Resorption.

Applications

Unspecified application

Species

Unspecified reactive species

Dongzheng Gai,Perry C Caviness,Oxana P Lazarenko,Jennifer F Chen,Christopher E Randolph,Zijun Zhang,Yan Cheng,Fumou Sun,Hongwei Xu,Michael L Blackburn,Guido Tricot,John D Shaughnessy,Jin-Ran Chen,Fenghuang Zhan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RSU1 antibody [EPR16127]

Immunoprecipitation - Anti-RSU1 antibody [EPR16127] (AB191409)

Western blot - Anti-RSU1 antibody [EPR16127] (AB191409)

Western blot - Anti-RSU1 antibody [EPR16127] (AB191409)

Western blot - Anti-RSU1 antibody [EPR16127] (AB191409)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise