Anti-RSU1 antibody [EPR16127] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-RSU1 antibody [EPR16127] - BSA and Azide free (AB251068)

This data was developed using ab191409, the same antibody clone in a different buffer formulation.

Immunoprecipitation analysis of Caco 2 cell lysate labeling RSU1 using ab191409 at 1/100 dilution (Lane 1). An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2 : PBS instead of Caco 2 cell lysate.

All lanes:

Immunoprecipitation - Anti-RSU1 antibody [EPR16127] (<a href='/en-us/products/primary-antibodies/rsu1-antibody-epr16127-ab191409'>ab191409</a>)

Predicted band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-RSU1 antibody [EPR16127] - BSA and Azide free (AB251068)

This data was developed using ab191409, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RSU1 antibody [EPR16127] (<a href='/en-us/products/primary-antibodies/rsu1-antibody-epr16127-ab191409'>ab191409</a>) at 1/10000 dilution

All lanes:

Caco-2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-RSU1 antibody [EPR16127] - BSA and Azide free (AB251068)

This data was developed using ab191409, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RSU1 antibody [EPR16127] (<a href='/en-us/products/primary-antibodies/rsu1-antibody-epr16127-ab191409'>ab191409</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Mouse heart tissue lysate at 10 µg

Lane 3:

Mouse kidney tissue lysate at 10 µg

Lane 4:

Mouse spleen tissue lysate at 10 µg

Lane 5:

Rat brain tissue lysate at 10 µg

Lane 6:

Rat heart tissue lysate at 10 µg

Lane 7:

Rat kidney tissue lysate at 10 µg

Lane 8:

Rat spleen tissue lysate at 10 µg

Lane 9:

C6 cell lysate at 10 µg

Lane 10:

Raw264.7 cell lysate at 10 µg

Lane 11:

PC12 cell lysate at 10 µg

Lane 12:

NIH 3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false

• WB

Supplier Data

Western blot - Anti-RSU1 antibody [EPR16127] - BSA and Azide free (AB251068)

This data was developed using ab191409, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RSU1 antibody [EPR16127] (<a href='/en-us/products/primary-antibodies/rsu1-antibody-epr16127-ab191409'>ab191409</a>) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Caco 2 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 32 kDa

false