AB99362

Anti-RTF1 antibody

Name: Anti-RTF1 antibody (ab99362) | Abcam
Brand: Abcam Limited
SKU: AB99362
Price: 545 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RTF1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human RTF1.

View Alternative Names

KIAA0252, RTF1, RNA polymerase-associated protein RTF1 homolog

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody (AB99362)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody (AB99362)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and mouse teratoma (right) tissues labelling RTF1 with ab99362 at 1/1000 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-RTF1 antibody (AB99362)

Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab99362 at 3 µg/mg lysate; 20% of immunoprecipitate loaded in lane Western Blot : RTF1 antibody (ab99362) at 1 µg/ml

developed using the ECL technique

Exposure time : 3 seconds

Predicted band size : 77 kDa

All lanes:

Immunoprecipitation - Anti-RTF1 antibody (ab99362)

Predicted band size: 80 kDa

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Unknown

Western blot - Anti-RTF1 antibody (AB99362)

All lanes:

Western blot - Anti-RTF1 antibody (ab99362) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 80 kDa

true

Exposure time: 10s

CiteAb

Western blot - Anti-RTF1 antibody (AB99362)

RTF1 western blot using anti-RTF1 antibody ab99362. Publication image and figure legend from Eirin, A., Zhu, X. Y., et al., 2016, Sci Rep, PubMed 27786293.

ab99362 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab99362 please see the product overview.

(A) Functional annotation clustering (using DAVID 6.7) of proteins excluded from EVs. 1 : Nucleolus; 2 : Nucleoplasm; 3 : RNA splicing; 4 : Nucleotide binding; 5 : Nucleolus; 6 : Histone modification; 7 : DNA binding; 8 : RNA binding; 9 : Apoptosis; 10 : FHA regulatory proteins; 11 : Isopeptide bond; 12 : N-acetyltransferase activity; 13 : GTPase regulator activity; 14 : ATP binding; 15 : RNA transport; 16 : DNA replication; 17 : Mitochondria; 18 : Ubiquitin protein activity; 19 : Transcription factors; 20 : Macromolecular complex assembly; 21 : Lipid synthesis; 22 : Vesicle-mediated transport; 23 : Transcription cofactor activity; 24 : Helicase activity; 25 : Regulation of cell death; 26 : Cell division; 27 : Regulation of transferase activity; 28 : DNA repair; 29 : Respiratory development; 30 : Eye development; 31 : Mitochondrial membrane; 32 : Protein transport; 33 : Transcription regulator activity; 34 : Signaling proteins; 35 : Transcription repressor activity; 36 : Zinc fingers; 37 : Organelle membrane; 38 : Protein kinase cascade; 39 : Vasculature development; 40 : Immune system development; 41 : MAPkinase signaling pathway; 42 : Endoplasmic reticulum; 43 : WD40 repeat region; 44 : Zinc finger; 45 : GTP binding; 46 : Magnesium ion binding; 47 : Cytoskeleton; 48 : Enzyme receptor signaling; 49 : Calcium ion binding; 50 : Spermatogenesis; 51 : Response to hormone stimulus; 52 : Cell migration; 53 : Cytoplasmic vesicle; 54 : Protein dimerization activity; 55 : Peptidase activity; 56 : Cell fraction. (B) Expression of the candidate proteins complement component (C2), vascular endothelial growth factor (VEGF), von Willebrand factor (vWF), RNA polymerase-associated protein RTF1 homolog (RTF1), and FOS-Like Antigen 1 (FOSL1) was in accordance to the proteomics findings.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human RTF1. The exact immunogen used to generate this antibody is proprietary information.

Q92541

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Medaka fish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Tilapia": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RTF1 also known as RNA polymerase II-associated factor 1 homolog is a subunit of the PAF1 complex involved in transcription regulation. The protein has a molecular mass of approximately 105 kDa and is expressed in various tissues including the brain heart and lung. Mechanically RTF1 interacts with the RNA polymerase II complex and facilitates transcription elongation by modifying chromatin structure and participating in histone modification. This action makes RTF1 integral in the regulation of gene expression.

Biological function summary

RNA polymerase II-associated factor 1 plays an important role by promoting transcriptional elongation mRNA processing and histone methylation. It is a critical part of the PAF1 complex which also includes CDC73 CTR9 LEO1 and PAF1 proteins. This association aids the modulation of gene expression by influencing the transition from transcription initiation to elongation and additionally affects RNA processing mechanisms through interaction with other protein factors facilitating the correct synthesis and maturation of mRNA.

Pathways

Several cellular processes involve RNA polymerase II-associated factor 1 including the Wnt signaling pathway and the histone modification pathway. The protein's participation in Wnt signaling impacts cellular proliferation and differentiation. In the context of histone modifications RTF1 is linked to the deposition of histone methylation marks working closely with related proteins like PAF1 and CDC73. These pathways highlight the interconnectedness of RTF1 within cellular machinery.

RTF1 alterations have associations with cancer and neurodegenerative diseases. Dysregulation of the PAF1 complex notably through changes in RTF1 activity has been observed in several cancers where abnormal transcriptional control is a feature. Neurodegenerative disorders also show a link with defects in RTF1 possibly due to impaired transcriptional regulation. In these conditions the interaction and co-regulation with proteins like CDC73 contribute to a deeper understanding of the pathogenesis involved.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both independently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Binds single-stranded DNA. Required for maximal induction of heat-shock genes. Required for the trimethylation of histone H3 'Lys-4' (H3K4me3) on genes involved in stem cell pluripotency; this function is synergistic with CXXC1 indicative for an involvement of a SET1 complex (By similarity).

See full target information RTF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 6:36120 PubMed27786293

2016

Comparative proteomic analysis of extracellular vesicles isolated from porcine adipose tissue-derived mesenchymal stem/stromal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Alfonso Eirin,Xiang-Yang Zhu,Amrutesh S Puranik,John R Woollard,Hui Tang,Surendra Dasari,Amir Lerman,Andre J van Wijnen,Lilach O Lerman

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-RTF1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTF1 antibody (AB99362)

Immunoprecipitation - Anti-RTF1 antibody (AB99362)

Western blot - Anti-RTF1 antibody (AB99362)

Western blot - Anti-RTF1 antibody (AB99362)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise