Rabbit Polyclonal RTKN/Rhotekin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RTKN aa 1-150.
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Publications
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- Biochemical genetics 61:2135-21482023RTKN2 Inhibits the Growth, Migration, Invasion and Glycolysis of Lung Adenocarcinoma Cells by Inactivating the NF-κB Signalling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNa Wang,Jinxiang WangPubMed 36952123
- Oncology reports 37:1227-12342016MicroRNA-152 inhibits tumor cell growth by directly targeting RTKN in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiejing Zhou,Yanjun Zhang,Yuhong Qi,Dequan Yu,Qiuju Shao,Jun LiangPubMed 27922690
- Oncology reports 35:2529-342016Inhibition of rhotekin exhibits antitumor effects in lung cancer cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RTKN aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BST9
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Target data
Function
Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures.
Alternative names
RTKN1, RTKN, Rhotekin
Recommended products
Rabbit Polyclonal RTKN/Rhotekin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RTKN aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RTKN aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BST9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Keep as concentrated solution.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Rhotekin also known as RTKN is a scaffold protein with a molecular mass of approximately 62 kDa. It interacts with Rho GTPases facilitating the assembly of signaling complexes. Expressed in various tissues RTKN displays high levels of expression in liver and kidney tissues. Alternative splicing of RTKN results in multiple transcript variants.
- Biological function summary
RTKN modulates several cellular processes by influencing cytoskeletal organization. This protein is a part of a Rho GTPase signaling complex that plays roles in cellular morphology and movement. It binds to activated Rho which leads to inhibition of Rho-associated kinase (ROCK) an important regulator of actin structures. This binding stabilizes RTKN's influence on cytoskeletal arrangement.
- Pathways
Cellular processes worldwide involve Rho GTPase signals where RTKN plays an important role. It links to the Wnt signaling pathway pivotal for cell proliferation and differentiation through its interaction with Dishevelled proteins. RTKN functions alongside related proteins like ROCK within these pathways. These interactions influence signal transduction and cellular responses.
- Associated diseases and disorders
RTKN shows associations with various cancers including gastric cancer through its interactions with aberrant Rho GTPase activity. Moreover RTKN has implications in neurodegenerative diseases like Alzheimer's often involving disrupted Rho signaling alongside proteins like tau. These connections highlight RTKN's importance in disease progression and make it a potential therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-RTKN/Rhotekin antibody (ab154954)
All lanes: Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/5000 dilution
Lane 1: Jurkat whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Lane 3: NCI-H929 whole cell lysate at 30 µg
Predicted band size: 63 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (ab154954)
Rhotekin-transfected (right) or untransfected (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Stained with ab154954 diluted at 1/500 (green). Nuclear staining using Hoechst 33342 (blue).
Western blot - Anti-RTKN/Rhotekin antibody (ab154954)
All lanes: Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/500 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: human Rhotekin-transfected 293T whole cell lysate at 30 µg
Predicted band size: 63 kDa
Western blot - Anti-RTKN/Rhotekin antibody (ab154954)
7.5% SDS PAGE
All lanes: Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/1000 dilution
All lanes: Jurkat whole cell lysate at 30 µg
Predicted band size: 63 kDa
Western blot - Anti-RTKN/Rhotekin antibody (ab154954)
7.5% SDS PAGE
All lanes: Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/10000 dilution
All lanes: mouse brain whole cell lysate at 20 µg
Predicted band size: 63 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (ab154954)
Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling RTKN/Rhotekin with ab154954 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).
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