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AB154954

Anti-RTKN/Rhotekin antibody

5

(1 Review)

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(3 Publications)

Rabbit Polyclonal RTKN/Rhotekin antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RTKN aa 1-150.

View Alternative Names

RTKN1, RTKN, Rhotekin

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (AB154954)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (AB154954)

Immunofluorescence analysis of paraformaldehyde-fixed A431 cells labeling RTKN/Rhotekin with ab154954 at 1/200 dilution (green). The image in the lower panel is costained with Hoechst 33342 (blue).

Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (AB154954)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-RTKN/Rhotekin antibody (AB154954)

Rhotekin-transfected (right) or untransfected (left) HeLa cells were fixed in ice-cold MeOH for 5 min. Stained with ab154954 diluted at 1/500 (green). Nuclear staining using Hoechst 33342 (blue).

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)
  • WB

Unknown

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/1000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 63 kDa

false

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)
  • WB

Supplier Data

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)

All lanes:

Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/5000 dilution

Lane 1:

Jurkat whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Lane 3:

NCI-H929 whole cell lysate at 30 µg

Predicted band size: 63 kDa

false

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)
  • WB

Unknown

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)

7.5% SDS PAGE

All lanes:

Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/10000 dilution

All lanes:

mouse brain whole cell lysate at 20 µg

Predicted band size: 63 kDa

false

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)
  • WB

Supplier Data

Western blot - Anti-RTKN/Rhotekin antibody (AB154954)

All lanes:

Western blot - Anti-RTKN/Rhotekin antibody (ab154954) at 1/500 dilution

Lane 1:

293T whole cell lysate at 30 µg

Lane 2:

human Rhotekin-transfected 293T whole cell lysate at 30 µg

Predicted band size: 63 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human RTKN aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q9BST9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Keep as concentrated solution.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rhotekin also known as RTKN is a scaffold protein with a molecular mass of approximately 62 kDa. It interacts with Rho GTPases facilitating the assembly of signaling complexes. Expressed in various tissues RTKN displays high levels of expression in liver and kidney tissues. Alternative splicing of RTKN results in multiple transcript variants.
Biological function summary

RTKN modulates several cellular processes by influencing cytoskeletal organization. This protein is a part of a Rho GTPase signaling complex that plays roles in cellular morphology and movement. It binds to activated Rho which leads to inhibition of Rho-associated kinase (ROCK) an important regulator of actin structures. This binding stabilizes RTKN's influence on cytoskeletal arrangement.

Pathways

Cellular processes worldwide involve Rho GTPase signals where RTKN plays an important role. It links to the Wnt signaling pathway pivotal for cell proliferation and differentiation through its interaction with Dishevelled proteins. RTKN functions alongside related proteins like ROCK within these pathways. These interactions influence signal transduction and cellular responses.

RTKN shows associations with various cancers including gastric cancer through its interactions with aberrant Rho GTPase activity. Moreover RTKN has implications in neurodegenerative diseases like Alzheimer's often involving disrupted Rho signaling alongside proteins like tau. These connections highlight RTKN's importance in disease progression and make it a potential therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates Rho signaling to activate NF-kappa-B and may confer increased resistance to apoptosis to cells in gastric tumorigenesis. May play a novel role in the organization of septin structures.
See full target information RTKN
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biochemical genetics 61:2135-2148 PubMed36952123

2023

RTKN2 Inhibits the Growth, Migration, Invasion and Glycolysis of Lung Adenocarcinoma Cells by Inactivating the NF-κB Signalling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Na Wang,Jinxiang Wang

Oncology reports 37:1227-1234 PubMed27922690

2016

MicroRNA-152 inhibits tumor cell growth by directly targeting RTKN in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiejing Zhou,Yanjun Zhang,Yuhong Qi,Dequan Yu,Qiuju Shao,Jun Liang

Oncology reports 35:2529-34 PubMed26935528

2016

Inhibition of rhotekin exhibits antitumor effects in lung cancer cells.

Applications

WB

Species

Unspecified reactive species

Weizhen Zhang,Zhenyu Liang,Jing Li
View all publications
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