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AB246911

Anti-RTN3/HAP antibody

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(1 Publication)

Rabbit Polyclonal RTN3/HAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RTN3 aa 50-250.

View Alternative Names

ASYIP, NSPL2, RTN3, Reticulon-3, Homolog of ASY protein, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II, HAP, NSP-like protein 2, NSP-like protein II, NSPLII

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-RTN3/HAP antibody (AB246911)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RTN3/HAP antibody (AB246911)

PFA-fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for RTN3/HAP using ab246911 (green) at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)

Paraffin-embedded human liver tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in hepatocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)

Paraffin-embedded human colon tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in glandular cells as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)

Paraffin-embedded human lymph node tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in lymphoid cells as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (AB246911)

Paraffin-embedded human cerebral cortex tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RTN3 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

O95197

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RTN3 also known as reticulon-3 or HAP (Hydroxy Alkenal Protection protein) is a member of the reticulon family of proteins which have roles in various cellular processes. This protein has a mass of approximately 130 kDa and is expressed in several tissues with significantly higher levels in the brain and spinal cord. RTN3 localizes to the endoplasmic reticulum where it participates in maintaining the structure of the tubular network influencing intracellular transport and signaling.
Biological function summary

The RTN3/HAP protein can influence neurite outgrowth implicating it in the development and maintenance of the nervous system. This protein associates closely with other members of the reticulon family forming complexes important for its functional roles within the cell. RTN3/HAP has been implicated in mechanisms that protect neurons from stress especially related to endoplasmic reticulum stress by its participation in membrane remodeling and regulation of lipid metabolism.

Pathways

Data shows that RTN3/HAP interacts with components of the endoplasmic reticulum stress response pathway and lipid biosynthesis pathways. It works alongside proteins such as RTN4/Nogo contributing to the regulation of axon guidance and regeneration. These pathways are essential for maintaining cellular homeostasis and responding to physiological stress with RTN3/HAP helping adapt or protect cells under challenging conditions.

Researchers have found associations between RTN3/HAP and neurodegenerative diseases like Alzheimer's disease where it is involved in pathologic processes such as amyloid-beta peptide aggregation. Aberrant expression or mutation of RTN3/HAP can disrupt normal protein turnover linking it to the pathophysiology of such diseases along with related proteins like presenilin and amyloid precursor protein (APP). Understanding these associations offers insights into therapeutic targets for managing these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. Induces the formation of endoplasmic reticulum tubules (PubMed : 25612671). Also acts as an inflammation-resolving regulator by interacting with both TRIM25 and RIGI, subsequently impairing RIGI 'Lys-63'-linked polyubiquitination leading to IRF3 and NF-kappa-B inhibition.. (Microbial infection) Plays a positive role in viral replication and pathogenesis of enteroviruses.
See full target information RTN3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 27: PubMed37144506

2023

MAGL regulates synovial macrophage polarization vis inhibition of mitophagy in osteoarthritic pain.

Applications

Unspecified application

Species

Unspecified reactive species

Chengyong Gu,Mo Chen,Xueyan Li,Dechun Geng,Chen Wang
View all publications
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