Rabbit Polyclonal RTN3/HAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RTN3 aa 50-250.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RTN3 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link O95197
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Target data
Function
May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. Induces the formation of endoplasmic reticulum tubules (PubMed:25612671). Also acts as an inflammation-resolving regulator by interacting with both TRIM25 and RIGI, subsequently impairing RIGI 'Lys-63'-linked polyubiquitination leading to IRF3 and NF-kappa-B inhibition. (Microbial infection) Plays a positive role in viral replication and pathogenesis of enteroviruses.
Alternative names
ASYIP, NSPL2, RTN3, Reticulon-3, Homolog of ASY protein, Neuroendocrine-specific protein-like 2, Neuroendocrine-specific protein-like II, HAP, NSP-like protein 2, NSP-like protein II, NSPLII
Recommended products
Rabbit Polyclonal RTN3/HAP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RTN3 aa 50-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RTN3 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link O95197
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RTN3 also known as reticulon-3 or HAP (Hydroxy Alkenal Protection protein) is a member of the reticulon family of proteins which have roles in various cellular processes. This protein has a mass of approximately 130 kDa and is expressed in several tissues with significantly higher levels in the brain and spinal cord. RTN3 localizes to the endoplasmic reticulum where it participates in maintaining the structure of the tubular network influencing intracellular transport and signaling.
- Biological function summary
The RTN3/HAP protein can influence neurite outgrowth implicating it in the development and maintenance of the nervous system. This protein associates closely with other members of the reticulon family forming complexes important for its functional roles within the cell. RTN3/HAP has been implicated in mechanisms that protect neurons from stress especially related to endoplasmic reticulum stress by its participation in membrane remodeling and regulation of lipid metabolism.
- Pathways
Data shows that RTN3/HAP interacts with components of the endoplasmic reticulum stress response pathway and lipid biosynthesis pathways. It works alongside proteins such as RTN4/Nogo contributing to the regulation of axon guidance and regeneration. These pathways are essential for maintaining cellular homeostasis and responding to physiological stress with RTN3/HAP helping adapt or protect cells under challenging conditions.
- Associated diseases and disorders
Researchers have found associations between RTN3/HAP and neurodegenerative diseases like Alzheimer's disease where it is involved in pathologic processes such as amyloid-beta peptide aggregation. Aberrant expression or mutation of RTN3/HAP can disrupt normal protein turnover linking it to the pathophysiology of such diseases along with related proteins like presenilin and amyloid precursor protein (APP). Understanding these associations offers insights into therapeutic targets for managing these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-RTN3/HAP antibody (ab246911)
PFA-fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells stained for RTN3/HAP using ab246911 (green) at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
Paraffin-embedded human cerebral cortex tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
Paraffin-embedded human colon tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in glandular cells as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
Paraffin-embedded human lymph node tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in lymphoid cells as expected.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RTN3/HAP antibody (ab246911)
Paraffin-embedded human liver tissue stained for RTN3/HAP using ab246911 at 1/50 dilution in immunohistochemical analysis. No positivity in hepatocytes as expected.
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