AB4751

Anti-RUB1 antibody

Name: Anti-RUB1 antibody (ab4751) | Abcam
Brand: Abcam Limited
SKU: AB4751
Price: 525 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal RUB1 antibody. Suitable for WB and reacts with Recombinant full length protein - Saccharomyces cerevisiae, Saccharomyces cerevisiae samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C RUB1.

View Alternative Names

YDR139C, YD9302.15C, NEDD8-like protein RUB1, Related to ubiquitin protein 1, Ubiquitin-like protein RUB1

2 Images

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Western blot - Anti-RUB1 antibody (AB4751)

ab4751 tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1 : 200 and 1 : 1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1 : 500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1 : 2000 dilution of Donkey polyclonal to Rabbit IgG (HRP) (ab7083) for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). ab4751 tested by immunoblot against a yeast cell lysate. A dilution of the antibody between 1 : 200 and 1 : 1,000 will show strong reactivity specifically with free Rub1 protein (indicated by arrow) and Rub1 conjugates. In this blot the antibody was used at a 1 : 500 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1 : 2000 dilution of Donkey polyclonal to Rabbit IgG (HRP) (ab7083) for 1 hour at r

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Western blot - Anti-RUB1 antibody (ab4751)

Predicted band size: 9 kDa

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Western blot - Anti-RUB1 antibody (AB4751)

Immunoblot of Rub1 fusion protein. Anti-Rub1 antibody generated by immunization with recombinant yeast Rub1 was tested by immunoblot against yeast lysates expressing the Rub1-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1 : 1,000 dilution of Goat polyclonal to GFP (ab6673) followed by reaction with a 1 : 15,000 dilution of Donkey polyclonal to Goat IgG (ab7125). Panel C shows specific reaction with Rub1 using a 1 : 1,000 dilution of ab4751 followed by reaction with a 1 : 15,000 dilution of Goat polyclonal to Rabbit IgG (HRP) (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat mi

All lanes:

Western blot - Anti-RUB1 antibody (ab4751)

Predicted band size: 9 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C RUB1.

Q03919

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Recombinant full length protein - Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification technique

Ion exchange chromatography

Purification notes

Rub1 Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like protein modifier that can be covalently attached to lysine residues of target proteins. Activated by the dimeric UBA3-ULA1 E1 enzyme and conjugated by the E2 UBC12 to substrate proteins. RUB1-conjugated (neddylated) substrate proteins include the cullins CDC53, RTT101 and CUL3, and the modification enhances the ubiquitin-ligase activity of the corresponding cullin-RING-based E3 ubiquitin-protein ligase complexes (CRLs).

See full target information RUB1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 44:68 PubMed39994761

2025

Reciprocal regulation of MMP-28 and EGFR is required for sustaining proliferative signaling in PDAC.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengtao Hong,Xing Huang,Linghao Xia,Tingbo Liang,Xueli Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RUB1 antibody

Western blot - Anti-RUB1 antibody (AB4751)

Western blot - Anti-RUB1 antibody (AB4751)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purity

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise