Mouse Monoclonal POLS antibody. Suitable for WB, I-ELISA and reacts with Rubella virus samples. Cited in 11 publications.
Images
![Western blot - Anti-Rubella Virus capsid antibody [9B11] (AB34749), expandable thumbnail](https://content.abcam.com/products/images/rubella-virus-capsid-antibody-9b11-ab34749--western-blot-img52583.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Rubella Virus capsid antibody [9B11] (AB34749)")
Publications
Filter by
- International journal of molecular sciences 23:2022Rubella Virus Triggers Type I Interferon Antiviral Response in Cultured Human Neural Cells: Involvement in the Control of Viral Gene Expression and Infectious Progeny Production.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSayuri Sakuragi,Huanan Liao,Kodai Yajima,Shigeyoshi Fujiwara,Hiroyuki NakamuraPubMed 36077193
- Frontiers in immunology 12:7960652021Rubella Virus Infected Macrophages and Neutrophils Define Patterns of Granulomatous Inflammation in Inborn and Acquired Errors of Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLudmila Perelygina,Raeesa Faisthalab,Emily Abernathy,Min-Hsin Chen,LiJuan Hao,Lionel Bercovitch,Diana K Bayer,Lenora M Noroski,Michael T Lam,Maria Pia Cicalese,Waleed Al-Herz,Arti Nanda,Joud Hajjar,Koen Vanden Driessche,Shari Schroven,Julie Leysen,Misha Rosenbach,Philipp Peters,Johannes Raedler,Michael H Albert,Roshini S Abraham,Hemalatha G Rangarjan,David Buchbinder,Lisa Kobrynski,Anne Pham-Huy,Julie Dhossche,Charlotte Cunningham Rundles,Anna K Meyer,Amy Theos,T Prescott Atkinson,Amy Musiek,Mehdi Adeli,Ute Derichs,Christoph Walz,Renate Krüger,Horst von Bernuth,Christoph Klein,Joseph Icenogle,Fabian Hauck,Kathleen E SullivanPubMed 35003119
- Microorganisms 9:2021The Epithelial-to-Mesenchymal Transition-Like Process Induced by TGF-β1 Enhances Rubella Virus Binding and Infection in A549 Cells via the Smad Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNgan Thi Kim Pham,Quang Duy Trinh,Kazuhide Takada,Chika Takano,Mari Sasano,Shoko Okitsu,Hiroshi Ushijima,Shihoko Komine-Aizawa,Satoshi HayakawaPubMed 33806778
- Genes to cells : devoted to molecular & cellular mechanisms 23:1023-10422018Albatross/FBF1 contributes to both centriole duplication and centrosome separation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkihito Inoko,Tomoki Yano,Tatsuo Miyamoto,Shinya Matsuura,Tohru Kiyono,Naoki Goshima,Masaki Inagaki,Yuko HayashiPubMed 30318703
- IEEE journal of solid-state circuits 53:2054-20642018High-Density Redox Amplified Coulostatic Discharge-Based Biosensor Array.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander C Sun et. al.PubMed 30559530
- Viruses 10:2018Myelin Oligodendrocyte Glycoprotein-Independent Rubella Infection of Keratinocytes and Resistance of First-Trimester Trophoblast Cells to Rubella Virus In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQuang Duy Trinh et. al.PubMed 29300335
- Clinical infectious diseases : an official publication of the Infectious Diseases Society of America 64:83-862016Cutaneous and Visceral Chronic Granulomatous Disease Triggered by a Rubella Virus Vaccine Strain in Children With Primary Immunodeficiencies.Applications:IHCReactive species:Unspecified reactive speciesBénédicte Neven,Philippe Pérot,Julie Bruneau,Marlene Pasquet,Marie Ramirez,Jean-Sébastien Diana,Stéphanie Luzi,Nicole Corre-Catelin,Christophe Chardot,Despina Moshous,Stéphanie Leclerc Mercier,Nizar Mahlaoui,Nathalie Aladjidi,Brigitte Le Bail,Marc Lecuit,Christine Bodemer,Thierry Jo Molina,Stéphane Blanche,Marc EloitPubMed 27810866
- The Journal of allergy and clinical immunology 138:1436-1439.e112016Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies.Applications:IFReactive species:Unspecified reactive speciesLudmila Perelygina et. al.PubMed 27613149
- Japanese journal of infectious diseases 69:350-12016Chance Isolation of Non-Pathogenic Vaccine-Derived Measles and Rubella Viruses from Children with Acute Respiratory Infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoko Aoki et. al.PubMed 26902222
- PloS one 10:e01332672015Differences in Establishment of Persistence of Vaccine and Wild Type Rubella Viruses in Fetal Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLudmila Perelygina et. al.PubMed 26177032
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | I-ELISA | |
|---|---|---|
| Rubella virus | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rubella virus | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
I-ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rubella virus | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Target data
Function
Capsid protein. Capsid protein interacts with genomic RNA and assembles into icosahedric core particles 65-70 nm in diameter (PubMed:28575072). The resulting nucleocapsid eventually associates with the cytoplasmic domain of E2 at the cell membrane, leading to budding and formation of mature virions from host Golgi membranes (PubMed:28575072). Phosphorylation negatively regulates RNA-binding activity, possibly delaying virion assembly during the viral replication phase. Capsid protein dimerizes and becomes disulfide-linked in the virion (PubMed:24282305). Modulates genomic RNA replication. Modulates subgenomic RNA synthesis by interacting with human C1QBP/SF2P32 (PubMed:10823864). Induces both perinuclear clustering of mitochondria and the formation of electron-dense intermitochondrial plaques, both hallmarks of rubella virus infected cells (PubMed:16051872). Induces apoptosis when expressed in transfected cells (PubMed:11017784). Spike glycoprotein E2. Responsible for viral attachment to target host cell, by binding to the cell receptor. Its transport to the plasma membrane depends on interaction with E1 protein. The surface glycoproteins display an irregular helical organization and a pseudo-tetrameric inner nucleocapsid arrangement (PubMed:28575072). Spike glycoprotein E1. Class II viral fusion protein (PubMed:23292515). Fusion activity is inactive as long as E1 is bound to E2 in mature virion. After virus attachment to target cell and clathrin-mediated endocytosis, acidification of the endosome would induce dissociation of E1/E2 heterodimer and concomitant trimerization of the E1 subunits (By similarity). This E1 homotrimer is fusion active, and promotes release of viral nucleocapsid in cytoplasm after endosome and viral membrane fusion (PubMed:9765418). The cytoplasmic tail of spike glycoprotein E1 modulates virus release (PubMed:10233921, PubMed:10708417). The surface glycoproteins display an irregular helical organization and a pseudo-tetrameric inner nucleocapsid arrangement (PubMed:28575072).
Additional Targets
POLS_RUBVT
Alternative names
Structural polyprotein, p110
Recommended products
Mouse Monoclonal POLS antibody. Suitable for WB, I-ELISA and reacts with Rubella virus samples. Cited in 11 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 9B11
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 28/11/2017. Lot numbers higher than GR120838-1 and GR210838-5 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Rubella Virus capsid is an important structural component of the Rubella virus particle. Sometimes called the capsid protein or capsid proteins it is involved in the assembling and packaging of the viral genome into new virions. The Rubella Virus capsid protein is around 33 kilodaltons in mass. It is expressed within infected host cells where it facilitates the initial steps of viral replication and particle formation. This capsid protein is essential for the structural integrity of the virus ensuring proper encapsulation of the viral RNA.
- Biological function summary
The Rubella Virus capsid interacts with various host cell factors to mediate processes like viral assembly and budding. It is part of a capsid protein complex which plays a role in the lifecycle of the virus by protecting the viral nucleic material. This nucleoprotein protein interacts with the host cell’s machinery influencing cell entry and immune evasion. The capsid elicits immune responses in the host which are detectable through assays such as the capsid ELISA often used in anti-rubella serological testing.
- Pathways
The Rubella Virus capsid is closely associated with the viral replication pathway and immune signaling pathways. It partners with other viral proteins to regulate replication ensuring successful synthesis of new virus particles. The host immune response against the capsid is significant involving pathways that trigger antibody production. The interaction of capsid proteins with host elements can modify immune responses assisting the virus in evading detection. Notable related proteins include the envelope proteins which work in tandem during the viral lifecycle.
- Associated diseases and disorders
The Rubella Virus capsid is fundamentally linked to rubella particularly congenital rubella syndrome. This capsid protein triggers immune responses that may not fully prevent infection but are important in diagnostic evaluations through tools like anti-rubella ELISA. Rubella infection can impact fetal development during pregnancy leading to severe outcomes. The interaction of the capsid with other viral components like the nucleoprotein protein is significant in the spread and pathology of the virus influencing the course of infectivity and disease manifestation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749), expandable thumbnail](https://content.abcam.com/products/images/rubella-virus-capsid-antibody-9b11-ab34749--western-blot-img52583.jpg "Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)")
Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749)
All lanes: Western blot - Anti-Rubella Virus capsid antibody [9B11] (ab34749) at 1 µg/mL
Lane 1: crude yeast cell lysate [mock control]
Lanes 2 - 3: irrelevant viral proteins
Lane 4: recombinant purified rubella capsid protein
Lane 5: crude lysate of transformed yeast cells expressing rubella capsid protein
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com