AB156052

Anti-Rubicon/Baron antibody

Name: Anti-Rubicon/Baron antibody (ab156052) | Abcam
Brand: Abcam Limited
SKU: AB156052
Price: 455 USD
Availability: InStock
Rating: 3 (5 reviews)

(5 Reviews)

(7 Publications)

Rabbit Polyclonal Rubicon/Baron antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 7 publications.

View Alternative Names

Kiaa0226, Rubcn, Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, Rubicon

2 Images

ICC/IF

Immunocytochemistry/ Immunofluorescence - Anti-Rubicon/Baron antibody (AB156052)

ICC/IF image of ab156052 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab156052 at 10μg/ml overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h.

ab50533 (Mouse monoclonal [Rab7-117] to RAB7) was also incubated along with ab156052 to demonstrate antigen co-localization. Signal detected using Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed (ab150120) (pseudo-colored red) DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

Lab

Western blot - Anti-Rubicon/Baron antibody (AB156052)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab156052 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-Rubicon/Baron antibody (ab156052) at 1 µg/mL

Lane 1:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 2:

Kidney (Mouse) Tissue Lysate at 10 µg

Lane 3:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Lane 4:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 109 kDa

Observed band size: 106 kDa,42 kDa,48 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Rubicon also known as Baron is a protein that functions mechanically to regulate autophagy and endosomal maturation. It has a molecular weight of approximately 70 kDa. You can find Rubicon expressed in various tissues but it is especially abundant in the liver spleen and pancreas. The protein acts by interacting with key autophagic and endocytic machinery including Rab7 and UVRAG which modulate vesicular trafficking processes vital for cellular homeostasis.

Biological function summary

Rubicon plays a role as part of the Beclin1-PI3K complex inhibiting autophagic activity. This protein modulates the fusion of autophagosomes with lysosomes a critical step in degrading cellular components. By preventing excessive autophagy Rubicon helps maintain cellular equilibrium balancing degradation and synthesis processes. Its involvement in endo-lysosomal trafficking is essential for the management of cellular waste and nutrient recycling.

Pathways

Rubicon has an important role in the PI3K/AKT signaling and autophagy pathways. Through its interaction with proteins like Beclin1 and Rab7 Rubicon becomes a significant modulator of autophagic flux influencing cellular growth responses and survival. The protein's regulatory function in these pathways impacts how cells respond to stress and nutrient availability linking metabolic demands with appropriate cellular responses.

Aberrant expression of Rubicon connects to cancer and neurodegenerative disorders including Alzheimer's disease. In cancer Rubicon's modulation of autophagy can influence tumor growth and resistance to apoptosis often working in conjunction with Beclin1 to impact tumor dynamics. In Alzheimer's disease the interaction of Rubicon with proteins involved in autophagy and endosomal pathways like Rab7 is associated with the accumulation of pathogenic proteins highlighting its potential role in disease pathogenesis and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process (PubMed : 19270693). Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (By similarity).. Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA : CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines. Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes (By similarity).

See full target information Rubcn

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7455 PubMed40796541

2025

Dual-targeted siRubicon delivery strategy triggers hepatocellular lipophagy for mitigating liver steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Lan,Qiushi Li,Mingxing Yu,Xu Duan,Tao Ming,Shuo Li,Chunjiong Wang,Yi Zhu,Zhongyang Shen,Deling Kong,Yang Liu

Journal of traditional and complementary medicine 15:434-445 PubMed40677544

2025

Salvianic acid A promotes osteogenic differentiation of bone marrow mesenchymal stem cells in senile osteoporosis through bromodomain-containing protein 4/Ariadne RBR E3 ubiquitin-protein ligase 1/Rubicon axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixian Xu,Jun Luo,Yongfa Zhang,Zhenyu Zhang,Jie Xu

Frontiers in immunology 14:1142492 PubMed36969163

2023

Human intestinal epithelial cells can internalize luminal fungi LC3-associated phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Sarit Cohen-Kedar,Efrat Shaham Barda,Keren Masha Rabinowitz,Danielle Keizer,Hanan Abu-Taha,Shoshana Schwartz,Kawsar Kaboub,Liran Baram,Eran Sadot,Ian White,Nir Wasserberg,Meirav Wolff-Bar,Adva Levy-Barda,Iris Dotan

Diabetes 70:562-576 PubMed33239448

2020

Podocyte EGFR Inhibits Autophagy Through Upregulation of Rubicon in Type 2 Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Yu Pan,Shirong Cao,Kensuke Sasaki,Yinqiu Wang,Aolei Niu,Xiaofeng Fan,Suwan Wang,Ming-Zhi Zhang,Raymond C Harris

Cell stress & chaperones 24:825-833 PubMed31183612

2019

Resveratrol, an activator of SIRT1, improves ER stress by increasing clusterin expression in HepG2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jinmi Lee,Seok-Woo Hong,Hyemi Kwon,Se Eun Park,Eun-Jung Rhee,Cheol-Young Park,Ki-Won Oh,Sung-Woo Park,Won-Young Lee

Nature microbiology 3:791-803 PubMed29849062

2018

Calcium sequestration by fungal melanin inhibits calcium-calmodulin signalling to prevent LC3-associated phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Kyrmizi,Helena Ferreira,Agostinho Carvalho,Julio Alberto Landero Figueroa,Pavlos Zarmpas,Cristina Cunha,Tonia Akoumianaki,Kostas Stylianou,George S Deepe,George Samonis,João F Lacerda,António Campos,Dimitrios P Kontoyiannis,Nikolaos Mihalopoulos,Kyung J Kwon-Chung,Jamel El-Benna,Isabel Valsecchi,Anne Beauvais,Axel A Brakhage,Nuno M Neves,Jean-Paul Latge,Georgios Chamilos

Oxidative medicine and cellular longevity 2018:4938649 PubMed29887941

2018

Phagocytosis of Inhibits Autophagic Flux in Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Duan,Qing Chen,Leilei Du,Jianbo Tong,Song Xu,Rong Zeng,Yuting Ma,Xu Chen,Min Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Rubicon/Baron antibody

Immunocytochemistry/ Immunofluorescence - Anti-Rubicon/Baron antibody (AB156052)

Western blot - Anti-Rubicon/Baron antibody (AB156052)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise