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Rabbit Recombinant Monoclonal Rubicon/Baron antibody. Suitable for WB and reacts with Human samples.

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Images

Western blot - Anti-Rubicon/Baron antibody [EPR26996-20] (AB307720), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Inhibits PIK3C3 activity; under basal conditions negatively regulates PI3K complex II (PI3KC3-C2) function in autophagy. Negatively regulates endosome maturation and degradative endocytic trafficking and impairs autophagosome maturation process. Can sequester UVRAG from association with a class C Vps complex (possibly the HOPS complex) and negatively regulates Rab7 activation (PubMed:20974968, PubMed:21062745). Involved in regulation of pathogen-specific host defense of activated macrophages. Following bacterial infection promotes NADH oxidase activity by association with CYBA thereby affecting TLR2 signaling and probably other TLR-NOX pathways. Stabilizes the CYBA:CYBB NADPH oxidase heterodimer, increases its association with TLR2 and its phagosome trafficking to induce antimicrobial burst of ROS and production of inflammatory cytokines (PubMed:22423966). Following fungal or viral infection (implicating CLEC7A (dectin-1)-mediated myeloid cell activation or RIGI-dependent sensing of RNA viruses) negatively regulates pro-inflammatory cytokine production by association with CARD9 and sequestering it from signaling complexes (PubMed:22423967).

Alternative names

KIAA0226, RUBCN, Run domain Beclin-1-interacting and cysteine-rich domain-containing protein, Rubicon, Beclin-1 associated RUN domain containing protein, Baron

Recommended products

Rabbit Recombinant Monoclonal Rubicon/Baron antibody. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26996-20
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Rubicon also known as Baron is a protein that functions mechanically to regulate autophagy and endosomal maturation. It has a molecular weight of approximately 70 kDa. You can find Rubicon expressed in various tissues but it is especially abundant in the liver spleen and pancreas. The protein acts by interacting with key autophagic and endocytic machinery including Rab7 and UVRAG which modulate vesicular trafficking processes vital for cellular homeostasis.

Biological function summary

Rubicon plays a role as part of the Beclin1-PI3K complex inhibiting autophagic activity. This protein modulates the fusion of autophagosomes with lysosomes a critical step in degrading cellular components. By preventing excessive autophagy Rubicon helps maintain cellular equilibrium balancing degradation and synthesis processes. Its involvement in endo-lysosomal trafficking is essential for the management of cellular waste and nutrient recycling.

Pathways

Rubicon has an important role in the PI3K/AKT signaling and autophagy pathways. Through its interaction with proteins like Beclin1 and Rab7 Rubicon becomes a significant modulator of autophagic flux influencing cellular growth responses and survival. The protein's regulatory function in these pathways impacts how cells respond to stress and nutrient availability linking metabolic demands with appropriate cellular responses.

Associated diseases and disorders

Aberrant expression of Rubicon connects to cancer and neurodegenerative disorders including Alzheimer's disease. In cancer Rubicon's modulation of autophagy can influence tumor growth and resistance to apoptosis often working in conjunction with Beclin1 to impact tumor dynamics. In Alzheimer's disease the interaction of Rubicon with proteins involved in autophagy and endosomal pathways like Rab7 is associated with the accumulation of pathogenic proteins highlighting its potential role in disease pathogenesis and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-Rubicon/Baron antibody [EPR26996-20] (ab307720), expandable thumbnail

    Western blot - Anti-Rubicon/Baron antibody [EPR26996-20] (ab307720)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Low expression: THP-1 (PMID: 22423966). In lane 1-3, the lysates were stored at -80°C prior to Western Blotting.

    The bands beneath the target band (150 kDa) are likely to be degradation products. In lanes 4-5, the lysates were freshly made and used for Western Blotting immediately to minimize protein degradation. In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-Rubicon/Baron antibody [EPR26996-20] (ab307720) at 1/1000 dilution

    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 2: K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 3: THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Lane 4: Raji whole cell lysate at 20 µg

    Lane 5: THP-1 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 150 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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