Rabbit Polyclonal RUNX1 / AML1 antibody. Suitable for WB and reacts with Human samples. Cited in 152 publications.
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- Journal of experimental & clinical cancer research : CR 42:2382023Combination of RUNX1 inhibitor and gemcitabine mitigates chemo-resistance in pancreatic ductal adenocarcinoma by modulating BiP/PERK/eIF2α-axis-mediated endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunhua She,Chao Wu,Weihua Guo,Yongjie Xie,Shouyi Li,Weishuai Liu,Chao Xu,Hui Li,Pei Cao,Yanfang Yang,Xiuchao Wang,Antao Chang,Yukuan Feng,Jihui HaoPubMed 37697370
- Biomolecules & biomedicine 23:405-4252023Identification of RUNX1 and IFNGR2 as prognostic-related biomarkers correlated with immune infiltration and subtype differentiation of low-grade glioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXia Zhang,Hongyu Chu,Yuan Cheng,Jie Ren,Wei Wang,Xicheng Liu,Xiaodong YanPubMed 36321611
- The EMBO journal 42:e1098032023Batf stabilizes Th17 cell development via impaired Stat5 recruitment of Ets1-Runx1 complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuy Pham,Daniel J Silberger,Kim N Nguyen,Min Gao,Casey T Weaver,Robin D HattonPubMed 36917143
- Cancer research 83:983-9962023SWI/SNF Blockade Disrupts PU.1-Directed Enhancer Programs in Normal Hematopoietic Cells and Acute Myeloid Leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCourtney Chambers,Katerina Cermakova,Yuen San Chan,Kristen Kurtz,Katharina Wohlan,Andrew Henry Lewis,Christiana Wang,Anh Pham,Milan Dejmek,Michal Sala,Mario Loeza Cabrera,Rogelio Aguilar,Radim Nencka,H Daniel Lacorazza,Rachel E Rau,H Courtney HodgesPubMed 36662812
- Molecules and cells 45:886-8952022RUNX1-Survivin Axis Is a Novel Therapeutic Target for Malignant Rhabdoid Tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasamitsu Mikami,Tatsuya Masuda,Takuya Kanatani,Mina Noura,Katsutsugu Umeda,Hidefumi Hiramatsu,Hirohito Kubota,Tomoo Daifu,Atsushi Iwai,Etsuko Yamamoto Hattori,Kana Furuichi,Saho Takasaki,Sunao Tanaka,Yasuzumi Matsui,Hidemasa Matsuo,Masahiro Hirata,Tatsuki R Kataoka,Tatsutoshi Nakahata,Yasumichi Kuwahara,Tomoko Iehara,Hajime Hosoi,Yoichi Imai,Junko Takita,Hiroshi Sugiyama,Souichi Adachi,Yasuhiko KamikuboPubMed 36572559
- Nature communications 13:71242022Regulome analysis in B-acute lymphoblastic leukemia exposes Core Binding Factor addiction as a therapeutic vulnerability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJason P Wray,Elitza M Deltcheva,Charlotta Boiers,Simon Е Richardson,Jyoti Bikram Chhetri,John Brown,Sladjana Gagrica,Yanping Guo,Anuradha Illendula,Joost H A Martens,Hendrik G Stunnenberg,John H Bushweller,Rachael Nimmo,Tariq EnverPubMed 36411286
- Bone research 10:632022Runx1 is a key regulator of articular cartilage homeostasis by orchestrating YAP, TGFβ, and Wnt signaling in articular cartilage formation and osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhang,Tao Zuo,Abigail McVicar,Hui-Lin Yang,Yi-Ping Li,Wei ChenPubMed 36307389
- Nature communications 13:61872022Runx2 and Runx3 differentially regulate articular chondrocytes during surgically induced osteoarthritis development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKosei Nagata,Hironori Hojo,Song Ho Chang,Hiroyuki Okada,Fumiko Yano,Ryota Chijimatsu,Yasunori Omata,Daisuke Mori,Yuma Makii,Manabu Kawata,Taizo Kaneko,Yasuhide Iwanaga,Hideki Nakamoto,Yuji Maenohara,Naohiro Tachibana,Hisatoshi Ishikura,Junya Higuchi,Yuki Taniguchi,Shinsuke Ohba,Ung-Il Chung,Sakae Tanaka,Taku SaitoPubMed 36261443
- Communications biology 5:9392022A RUNX-targeted gene switch-off approach modulates the BIRC5/PIF1-p21 pathway and reduces glioblastoma growth in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEtsuko Yamamoto Hattori,Tatsuya Masuda,Yohei Mineharu,Masamitsu Mikami,Yukinori Terada,Yasuzumi Matsui,Hirohito Kubota,Hidemasa Matsuo,Masahiro Hirata,Tatsuki R Kataoka,Tatsutoshi Nakahata,Shuji Ikeda,Susumu Miyamoto,Hiroshi Sugiyama,Yoshiki Arakawa,Yasuhiko KamikuboPubMed 36085167
- iScience 25:1049362022Activation of GDNF-ERK-Runx1 signaling contributes to P2X3R gene transcription and bone cancer pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhu-Lin Yuan,Xiao-Dan Liu,Zi-Xian Zhang,Song Li,Yue Tian,Ke Xi,Jie Cai,Xiao-Mei Yang,Min Liu,Guo-Gang XingPubMed 36072549
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
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Target data
Function
Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed:17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity). Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Isoform AML-1L interferes with the transactivation activity of RUNX1.
Alternative names
AML1, CBFA2, RUNX1, Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, CBF-alpha-2, PEA2-alpha B, PEBP2-alpha B
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Rabbit Polyclonal RUNX1 / AML1 antibody. Suitable for WB and reacts with Human samples. Cited in 152 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Antibody batches of a concentration <1mg/ml will have BSA added to them.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RUNX1 also known as AML1 is a transcription factor with a molecular weight of approximately 48 kDa. It belongs to the Runt-related transcription factor family and plays a critical role in hematopoiesis. RUNX1 is expressed in hematopoietic stem cells and various other tissues where it regulates the expression of genes involved in the differentiation and proliferation of blood cells. It exerts its function by binding to specific DNA sequences thereby controlling the transcriptional activity necessary for normal hematopoietic development.
- Biological function summary
RUNX1 is essential in the formation of blood cells and is part of the core-binding factor (CBF) complex. This complex is a heterodimer comprising RUNX1 and the CBFβ subunit. The interaction between RUNX1 and CBFβ stabilizes the DNA binding capability of RUNX1 facilitating the activation of target gene transcription. The proper functioning of RUNX1 is necessary for the maintenance of normal lineage specification of hematopoietic progenitors affecting both myeloid and lymphoid cell lineages.
- Pathways
RUNX1 plays a significant role in the Wnt signaling pathway and the TGF-beta signaling pathway. RUNX1 interacts with several proteins in these pathways including SMAD proteins and β-catenin which are important for transmitting extracellular signals that regulate cell growth and differentiation. RUNX1’s role in these pathways highlights its importance not only in hematopoiesis but also in preventing abnormal cell proliferation.
- Associated diseases and disorders
RUNX1 mutations are strongly associated with acute myeloid leukemia (AML) and familial platelet disorder. In AML RUNX1 mutations disrupt normal hematopoiesis leading to the uncontrolled proliferation of immature blood cells. RUNX1-related proteins such as the GM-CSF receptor can contribute to disease progression by altering cytokine signaling. RUNX1's involvement in familial platelet disorder reflects its importance in maintaining normal blood cell counts and function as loss of RUNX1 function leads to predisposition to leukemia.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-RUNX1 / AML1 antibody (ab23980)
This antibody recognized three distinct bands of between 48 and 55 kDa in Jurkat nuclear lysate. These may represent distinct isoforms of Runx1 or may represent post-translationally modified forms.
All lanes: Western blot - Anti-RUNX1 / AML1 antibody (ab23980) at 1 µg/mL
All lanes: Jurkat nuclear extract lysate (ab14844) at 20 µg
SecondaryAll lanes: Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Predicted band size: 48 kDa
Observed band size: 48 kDa, 52 kDa, 55 kDa
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