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Rabbit Recombinant Monoclonal RUNX1 / AML1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytIPIHC-PWB
Human
Not recommended
Not recommended
Not recommended
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Expected
Tested

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed:17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed:10207087, PubMed:14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed:17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity). Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation. Isoform AML-1L interferes with the transactivation activity of RUNX1.

Additional Targets

RUNX3, RUNX2

Alternative names

AML1, CBFA2, RUNX1, Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, CBF-alpha-2, PEA2-alpha B, PEBP2-alpha B

Recommended products

Rabbit Recombinant Monoclonal RUNX1 / AML1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR3098
Purification technique
Affinity purification Protein A
Specificity

The immunogen of this antibody is 100% homologous to mouse RUNX2 sequence.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248432 is the carrier-free version of Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] ab133261.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

RUNX1 also known as AML1 is a transcription factor that plays an important role in gene regulation. RUNX3 and RUNX2 are related proteins that belong to the same RUNX family which are defined by the runt homology domain. RUNX1 typically has a mass of approximately 50 kilodaltons (kDa). These proteins express in various tissues including hematopoietic cells for RUNX1 skeletal tissues for RUNX2 and immune cells for RUNX3. They bind to DNA influencing the transcription of target genes which are critical for development processes.

Biological function summary

RUNX proteins mediate cellular differentiation and proliferation. They often participate in complex with CBFβ which stabilizes their interaction with DNA and enhances transcriptional activity. RUNX1 in particular regulates hematopoiesis the process of blood cell formation. RUNX2 is fundamentally involved in osteogenesis facilitating bone growth and remodeling. RUNX3 contributes to immune responses by affecting T-cell differentiation and function. Their dynamic interactions dictate the expression levels of genes involved in these vital processes.

Pathways

RUNX proteins integrate into significant signaling cascades. In hematopoietic regulation RUNX1 interacts importantly within the Notch and TGF-β pathways. It cooperates with other proteins such as GATA1 and TAL1 influencing lineage commitment and differentiation of blood cells. RUNX2 critical in the ossification pathway often partners with proteins including SMADs in response to bone morphogenetic protein (BMP) signaling guiding osteoblast differentiation. These pathways demonstrate the versatility and specificity of RUNX proteins in biological processes.

Associated diseases and disorders

Aberrations in RUNX1 are strongly linked to acute myeloid leukemia (AML). Mutations or translocations affecting RUNX1 disrupt normal hematopoiesis leading to leukemogenic processes. RUNX3 involvement can be observed in cancers like gastric cancer where its silencing or inactivation associates with tumorigenesis. RUNX2 overexpression sometimes links with bone disorders such as cleidocranial dysplasia. Here RUNX2 may interact with other skeletal regulators like PTHLH influencing proper bone development and growth. Understanding these interactions provides insights into potential therapeutic targets for managing these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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