AB248432

Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal RUNX1 / AML1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.

View Alternative Names

AML1, CBFA2, RUNX1, Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit, CBF-alpha-2, PEA2-alpha B, PEBP2-alpha B, AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A, AML2, CBFA3, PEBP2A3, RUNX3, Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, CBF-alpha-3, PEA2-alpha C, PEBP2-alpha C

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human heart tissue using ab133261 showing -ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human testis tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labelling RUNX1 / AML1 with ab133261 at 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human breast tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab133261 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Lab

Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (<a href='/en-us/products/primary-antibodies/runx1-aml1-runx3-runx2-antibody-epr3098-ab133261'>ab133261</a>) at 1/1000 dilution

Lane 1:

Myc-DDK-tagged Human RUNX1 recombinant protein at 20 ng

Lane 2:

His-tagged Human RUNX2 recombinant protein at 20 ng

Lane 3:

His-tagged Human RUNX3 recombinant protein at 20 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 48.7 kDa

false

Exposure time: 5s

Unknown

Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

This data was developed using ab133261, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098] (<a href='/en-us/products/primary-antibodies/runx1-aml1-runx3-runx2-antibody-epr3098-ab133261'>ab133261</a>) at 1/1000 dilution

Lane 1:

Molt 4 cell lysate at 10 µg

Lane 2:

Human fetal thymus lysate at 10 µg

Lane 3:

Mouse thymus lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 48 kDa

false

Unconjugated

Anti-RUNX1 / AML1 + RUNX3 + RUNX2 antibody [EPR3098]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3098

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The immunogen of this antibody is 100% homologous to mouse RUNX2 sequence.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab248432 is the carrier-free version of ab133261.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RUNX1 also known as AML1 is a transcription factor that plays an important role in gene regulation. RUNX3 and RUNX2 are related proteins that belong to the same RUNX family which are defined by the runt homology domain. RUNX1 typically has a mass of approximately 50 kilodaltons (kDa). These proteins express in various tissues including hematopoietic cells for RUNX1 skeletal tissues for RUNX2 and immune cells for RUNX3. They bind to DNA influencing the transcription of target genes which are critical for development processes.

Biological function summary

RUNX proteins mediate cellular differentiation and proliferation. They often participate in complex with CBFβ which stabilizes their interaction with DNA and enhances transcriptional activity. RUNX1 in particular regulates hematopoiesis the process of blood cell formation. RUNX2 is fundamentally involved in osteogenesis facilitating bone growth and remodeling. RUNX3 contributes to immune responses by affecting T-cell differentiation and function. Their dynamic interactions dictate the expression levels of genes involved in these vital processes.

Pathways

RUNX proteins integrate into significant signaling cascades. In hematopoietic regulation RUNX1 interacts importantly within the Notch and TGF-β pathways. It cooperates with other proteins such as GATA1 and TAL1 influencing lineage commitment and differentiation of blood cells. RUNX2 critical in the ossification pathway often partners with proteins including SMADs in response to bone morphogenetic protein (BMP) signaling guiding osteoblast differentiation. These pathways demonstrate the versatility and specificity of RUNX proteins in biological processes.

Aberrations in RUNX1 are strongly linked to acute myeloid leukemia (AML). Mutations or translocations affecting RUNX1 disrupt normal hematopoiesis leading to leukemogenic processes. RUNX3 involvement can be observed in cancers like gastric cancer where its silencing or inactivation associates with tumorigenesis. RUNX2 overexpression sometimes links with bone disorders such as cleidocranial dysplasia. Here RUNX2 may interact with other skeletal regulators like PTHLH influencing proper bone development and growth. Understanding these interactions provides insights into potential therapeutic targets for managing these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). Essential for the development of normal hematopoiesis (PubMed : 17431401). Acts synergistically with ELF4 to transactivate the IL-3 promoter and with ELF2 to transactivate the BLK promoter (PubMed : 10207087, PubMed : 14970218). Inhibits KAT6B-dependent transcriptional activation (By similarity). Involved in lineage commitment of immature T cell precursors. CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity). Controls the anergy and suppressive function of regulatory T-cells (Treg) by associating with FOXP3. Activates the expression of IL2 and IFNG and down-regulates the expression of TNFRSF18, IL2RA and CTLA4, in conventional T-cells (PubMed : 17377532). Positively regulates the expression of RORC in T-helper 17 cells (By similarity).. Isoform AML-1G shows higher binding activities for target genes and binds TCR-beta-E2 and RAG-1 target site with threefold higher affinity than other isoforms. It is less effective in the context of neutrophil terminal differentiation.. Isoform AML-1L interferes with the transactivation activity of RUNX1.

See full target information RUNX1

Additional targets

RUNX3,RUNX2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Western blot - Anti-RUNX1 / AML1+RUNX3+RUNX2 antibody [EPR3098] - BSA and Azide free (AB248432)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

Product promise