Anti-RUNX2 antibody [2B9] is a mouse monoclonal antibody designed to detect RUNX2, a transcription factor essential for osteoblastic differentiation and skeletal morphogenesis. Validated for immunocytochemistry/immunofluorescence (ICC/IF), western blot (WB), and flow cytometry (Flow Cyt). Reacts with human, rat and more samples.
- Cited in over 320 publications
- Trusted by researchers since 2009
- Immunogen corresponding to Recombinant Fragment Protein within Human RUNX2 aa 250-400
Images
![Flow Cytometry - Anti-RUNX2 antibody [2B9] (AB76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--flow-cytometry-img50461.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-RUNX2 antibody [2B9] (AB76956)")
![Western blot - Anti-RUNX2 antibody [2B9] (AB76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--western-blot-img160189.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RUNX2 antibody [2B9] (AB76956)")
![Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (AB76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--immunocytochemistry-immunofluorescence-img61554.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (AB76956)")
![Western blot - Anti-RUNX2 antibody [2B9] (AB76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--western-blot-img150802.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-RUNX2 antibody [2B9] (AB76956)")
Publications
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- Journal of orthopaedic surgery and research 18:8762023BRD4 facilitates osteogenic differentiation of human bone marrow mesenchymal stem cells through WNT4/NF-κB pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Ning,Huihui Guo,Mingming Ma,Zhengang ZhaPubMed 37980502
- Arthritis research & therapy 25:1942023Preosteoclast plays a pathogenic role in syndesmophyte formation of ankylosing spondylitis through the secreted PDGFB - GRB2/ERK/RUNX2 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulong Tang,Kai Yang,Qingmei Liu,Yanyun Ma,Hao Zhu,Kunhai Tang,Chengchun Geng,Jiangnan Xie,Dachun Zhuo,Wenyu Wu,Li Jin,Wenze Xiao,Jiucun Wang,Qi Zhu,Jing LiuPubMed 37798786
- iScience 26:1079232023Circ_0000888 regulates osteogenic differentiation of periosteal mesenchymal stem cells in congenital pseudarthrosis of the tibia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuoyang Li,Yaoxi Liu,Yiyong Huang,Qian Tan,Haibo Mei,Guanghui Zhu,Kun Liu,Ge YangPubMed 37810257
- Journal of vascular research 60:193-2032023Emodin Inhibits the Indoxyl Sulfate-Induced trans-Differentiation of Vascular Smooth Muscle Cells through Upregulating Thrombospondin-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeidong Zhang,Jing Chen,Xiao Tan,Pan Zhang,Xialian Xu,Xiaoqiang Ding,Shuan Zhao,Shi JinPubMed 37669629
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:1480-14962023GSTT1 as a Predictive Marker and Enhancer for Osteogenic Potential of Human Adipose-Derived Stromal/Stem Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEugene Lee,Jae-Yeon Moon,Ji-Yun Ko,Seo-Young Park,Gun-Il ImPubMed 37537994
- Journal of orthopaedic translation 41:33-412023Disruption of Col9a2 expression leads to defects in osteochondral homeostasis and osteoarthritis-like phenotype in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Dong,Huihui Xu,Pinger Wang,Liang Fang,Luwei Xiao,Shuaijie Lv,Peijian Tong,Hongting JinPubMed 37635809
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229872023Hmga1-overexpressing lentivirus protects against osteoporosis by activating the Wnt/β-catenin pathway in the osteogenic differentiation of BMSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhixin Wu,Jiayong Zhu,Yinxian Wen,Pengfei Lei,Jie Xie,Haifei Shi,Ronghuan Wu,Xianfeng Lou,Yihe HuPubMed 37555233
- Journal of nanobiotechnology 21:2262023Cold exposure protects against medial arterial calcification development via autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFu-Xing-Zi Li,Jun-Jie Liu,Feng Xu,Su-Kang Shan,Ming-Hui Zheng,Li-Min Lei,Xiao Lin,Bei Guo,Chang-Chun Li,Feng Wu,Ke-Xin Tang,Ye-Chi Cao,Yun-Yun Wu,Jia-Yue Duan,Yan-Lin Wu,Si-Yang He,Xi Chen,Ling-Qing YuanPubMed 37461031
- Nature communications 14:40912023In situ activation of flexible magnetoelectric membrane enhances bone defect repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Liu,Han Zhao,Chenguang Zhang,Shiqi Xu,Fengyi Zhang,Ling Wei,Fangyu Zhu,Ying Chen,Yumin Chen,Ying Huang,Mingming Xu,Ying He,Boon Chin Heng,Jinxing Zhang,Yang Shen,Xuehui Zhang,Houbing Huang,Lili Chen,Xuliang DengPubMed 37429900
- International journal of molecular sciences 24:2023Accelerated Bone Loss in Transgenic Mice Expressing Constitutively Active TGF-β Receptor Type I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesParichart Toejing,Nithidol Sakunrangsit,Pinyada Pho-On,Chinnatam Phetkong,Asada Leelahavanichkul,Somyoth Sridurongrit,Matthew B Greenblatt,Sutada LotinunPubMed 37445982
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human RUNX2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q13950
Consider this alternative
Reactivity data
Select an application| Flow Cyt | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected |
| Chimpanzee | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended |
| Rhesus monkey | Predicted | Predicted | Predicted |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog, Pig, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human, Rat | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog, Pig, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Dog, Pig, Chimpanzee, Rhesus monkey | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed:28505335, PubMed:28703881, PubMed:28738062). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
Alternative names
AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A
Recommended products
Anti-RUNX2 antibody [2B9] is a mouse monoclonal antibody designed to detect RUNX2, a transcription factor essential for osteoblastic differentiation and skeletal morphogenesis. Validated for immunocytochemistry/immunofluorescence (ICC/IF), western blot (WB), and flow cytometry (Flow Cyt). Reacts with human, rat and more samples.
- Cited in over 320 publications
- Trusted by researchers since 2009
- Immunogen corresponding to Recombinant Fragment Protein within Human RUNX2 aa 250-400
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human RUNX2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q13950
- Carrier free
- Yes
- Clone number
- 2B9
- Purification technique
- Affinity purification Protein A
- Light chain type
- kappa
- Concentration
- Purification notes
Purified from Cell Culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Product SpecificationsAnti-RUNX2 antibody [2B9] (ab76956) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, WB in human, rat, samples.
Anti-RUNX2 antibody [2B9] (ab76956) specifically detects RUNX2 (UniProt ID: Q13950; Molecular weight: 57kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-RUNX2 antibody [2B9] (ab76956) has high sensitivity and specificity.
Anti-RUNX2 antibody [2B9] (ab76956) has been cited over 324 times in peer reviewed journals and is trusted by the scientific community.
Anti-RUNX2 antibody [2B9] (ab76956) has 7 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-RUNX2 antibody [2B9] (ab76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--flow-cytometry-img50461.jpg "Flow Cytometry - Anti-RUNX2 antibody [2B9] (ab76956)")
Flow Cytometry - Anti-RUNX2 antibody [2B9] (ab76956)
Overlay histogram showing Saos 2 cells stained with ab76956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76956, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (Mouse IgG2a, Kappa Monoclonal [B12/8] - Isotype Control ab91361, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
![Western blot - Anti-RUNX2 antibody [2B9] (ab76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--western-blot-img160189.jpg "Western blot - Anti-RUNX2 antibody [2B9] (ab76956)")
Western blot - Anti-RUNX2 antibody [2B9] (ab76956)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-RUNX2 antibody [2B9] (ab76956) at 1 µg/mL
All lanes: Recombinant tagged human RUNX2 fragment at 0.2 µg
SecondaryAll lanes: Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size: 57 kDa
Observed band size: 37 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (ab76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--immunocytochemistry-immunofluorescence-img61554.jpg "Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (ab76956)")
Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (ab76956)
ab76956 at 10μg/ml staining RUNX2 in HeLa cells by Immunofluorescence.
This image was generated using the ascites version of the product.
![Western blot - Anti-RUNX2 antibody [2B9] (ab76956), expandable thumbnail](https://content.abcam.com/products/images/runx2-antibody-2b9-ab76956--western-blot-img150802.jpg "Western blot - Anti-RUNX2 antibody [2B9] (ab76956)")
Western blot - Anti-RUNX2 antibody [2B9] (ab76956)
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-RUNX2 antibody [2B9] (ab76956) at 1 µg/mL
All lanes: PC12 cell lysate at 25 µg
SecondaryAll lanes: Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 57 kDa
Observed band size: 56 kDa
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