AB76956

Anti-RUNX2 antibody [2B9]

Name: Anti-RUNX2 antibody [2B9] Mouse monoclonal (ab76956) | Abcam
Brand: Abcam Limited
SKU: AB76956
Price: 470 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(409 Publications)

Anti-RUNX2 antibody [2B9] (ab76956) is a mouse monoclonal antibody detecting RUNX2 in Western Blot, Flow Cytometry, ICC/IF. Suitable for Human, Rat,.

- Over 320 publications
- Trusted since 2009

View Alternative Names

AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A

4 Images

Flow Cytometry - Anti-RUNX2 antibody [2B9] (AB76956)

Flow Cyt

Unknown

Flow Cytometry - Anti-RUNX2 antibody [2B9] (AB76956)

Overlay histogram showing Saos 2 cells stained with ab76956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76956, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

This image was generated using the ascites version of the product.

Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (AB76956)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (AB76956)

ab76956 at 10μg/ml staining RUNX2 in HeLa cells by Immunofluorescence.

This image was generated using the ascites version of the product.

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Western blot - Anti-RUNX2 antibody [2B9] (AB76956)

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-RUNX2 antibody [2B9] (ab76956) at 1 µg/mL

All lanes:

PC12 cell lysate at 25 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 57 kDa

Observed band size: 56 kDa

false

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Western blot - Anti-RUNX2 antibody [2B9] (AB76956)

Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 37 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-RUNX2 antibody [2B9] (ab76956) at 1 µg/mL

All lanes:

Recombinant tagged human RUNX2 fragment at 0.2 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

Predicted band size: 57 kDa

Observed band size: 37 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2B9

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat, Human

Applications

ICC/IF, Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human RUNX2 aa 250-400. The exact immunogen used to generate this antibody is proprietary information.

Q13950

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg2c-kappa-monoclonal-18c8bc7ad10-isotype-control-ab170191'>ab170191</a> - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Product Specifications
Anti-RUNX2 antibody [2B9] (ab76956) is a mouse monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, WB in human, rat, samples.
Anti-RUNX2 antibody [2B9] (ab76956) specifically detects RUNX2 (UniProt ID: Q13950; Molecular weight: 57kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-RUNX2 antibody [2B9] (ab76956) has high sensitivity and specificity.
Anti-RUNX2 antibody [2B9] (ab76956) has been cited over 324 times in peer reviewed journals and is trusted by the scientific community.
Anti-RUNX2 antibody [2B9] (ab76956) has 7 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from Cell Culture supernatant.

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed : 28505335, PubMed : 28703881, PubMed : 28738062). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation : synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.

See full target information RUNX2

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Publications (409)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 13:1639117 PubMed40837020

2025

Ipriflavone ameliorates intervertebral disc degeneration by inhibiting osteoporosis of vertebral body and pyroptosis of the nucleus pulposus in instability of lumbar spine and diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Hai-Jing Zhang,Hui Wang,Gang Ji,Ya-Heng Zhao,Gao-Cen Li,Shao-Shi Guo,Lu-Feng Lin,Yu-Jie Jin,Xue-Li Zhang,Xin-Yu Nan,Chang-Cheng Liu,Guo-Bin Liu

Materials today. Bio 34:102144 PubMed40791800

2025

Study on mRNA delivery via GelMA hydrogel-encapsulated extracellular vesicles for enhanced bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Li,Yueyang Sheng,Yi You,Ying Wang,Yanzhuo Zhang,Jianfeng Tao,Chengai Wu,Xu Jiang

Small science 5:2400468 PubMed40657193

2025

Magnetoactive Nanotopography on Hydrogels for Stimulated Cell Adhesion and Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Md Shariful Islam,Thomas G Molley,Gagan K Jalandhra,Jason Fang,Jamie J Kruzic,Kristopher A Kilian

Journal of cellular and molecular medicine 29:e70703 PubMed40629256

2025

Hypoxia-Driven Regulation of Osteogenic Differentiation in Human Periosteal Stem Cells via the HIF-1α/miR-129-5p/BMP2 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Lu,Xinyu Wang,Nan Lu,Aimin Chen,Lianbo Xiao

Nature communications 16:6200 PubMed40615395

2025

Divide-and-conquer strategy with engineered ossification center organoids for rapid bone healing through developmental cell recruitment.

Applications

Unspecified application

Species

Unspecified reactive species

Xianzhu Zhang,Wei Jiang,Xinyu Wu,Chang Xie,Yi Zhang,Liying Li,Yuqing Gu,Zihao Hu,Xinrang Zhai,Renjie Liang,Tao Zhang,Wei Sun,Jinchun Ye,Wei Wei,Xiaozhao Wang,Yi Hong,Shufang Zhang,Youzhi Cai,Xiaohui Zou,Yihe Hu,Hongwei Ouyang

Cell biology and toxicology 41:99 PubMed40493284

2025

SENP3 alleviates osteoporosis via promoting SIRT3 transcription through the increase of DLX2 stability via SUMO2/3.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Bu,Xuezheng Xu,Yi Luo,Jianfan Liu,Feng Zhou

Scientific reports 15:19979 PubMed40481141

2025

EIF4A3 enhances the viability, invasion and osteogenic differentiation of BMSCs via the USP53/SMAD5 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Cheng,Xiaoling Yang,Yongtao Tang,Changjun Wu,Zulin Tang,Siqi Wei

Medicina (Kaunas, Lithuania) 61: PubMed40428778

2025

Investigating the Associations Between Hmga2 Overexpression, R-Loop Reduction, and Bone Loss in Aging Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yangyang Cao,Yantong Wang,Dengsheng Xia

Folia histochemica et cytobiologica 63:65-78 PubMed40421821

2025

MiR-494-3p knockdown promotes osteogenic differentiation of bone marrow mesenchymal stem cells in ovariectomised rats by targeting Sirt1/TLR4/NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianping Lei,Song Guo,Jiabing Kuang,Bo Shen,Zixi Li

Bioactive materials 49:608-626 PubMed40212781

2025

Continuous mechanical-gradient hydrogel with on-demand distributed Mn/Mg-doped hydroxyapatite@FeO for functional osteochondral regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Junwei Xu,Yi Cui,Ping Li,Xuemei Sun,Zhiheng Chen,Jingxi Wang,Xuenan Gu,Xiaogang Wang,Yubo Fan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-RUNX2 antibody [2B9]

Flow Cytometry - Anti-RUNX2 antibody [2B9] (AB76956)

Immunocytochemistry/ Immunofluorescence - Anti-RUNX2 antibody [2B9] (AB76956)

Western blot - Anti-RUNX2 antibody [2B9] (AB76956)

Western blot - Anti-RUNX2 antibody [2B9] (AB76956)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (409)

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