Rabbit Polyclonal RUNX2 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 15 publications.
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- Nature communications 14:40912023In situ activation of flexible magnetoelectric membrane enhances bone defect repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Liu,Han Zhao,Chenguang Zhang,Shiqi Xu,Fengyi Zhang,Ling Wei,Fangyu Zhu,Ying Chen,Yumin Chen,Ying Huang,Mingming Xu,Ying He,Boon Chin Heng,Jinxing Zhang,Yang Shen,Xuehui Zhang,Houbing Huang,Lili Chen,Xuliang DengPubMed 37429900
- Human cell 36:1389-14022023Effect of psoralen on the regulation of osteogenic differentiation induced by periodontal stem cell-derived exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Yu,Xiaonan Wu,Wenyi Zhang,Fuhang Chu,Qi Zhang,Meihua Gao,Yingjie Xu,Yingtao WuPubMed 37269415
- Molecular biology reports 50:4769-47792023Runx2 overexpression promotes bone repair of osteonecrosis of the femoral head (ONFH).Applications:Unspecified applicationReactive species:Unspecified reactive speciesHai-Jia Xu,Xiang-Zhong Liu,Lu Yang,Yu Ning,Liang-Liang Xu,Da-Ming Sun,Wen Liao,Yi Yang,Zhang-Hua LiPubMed 37029290
- Matrix biology : journal of the International Society for Matrix Biology 119:19-562023Phase-specific signatures of wound fibroblasts and matrix patterns define cancer-associated fibroblast subtypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMateusz S Wietecha,David Lauenstein,Michael Cangkrama,Sybille Seiler,Juyoung Jin,Andreas Goppelt,Manfred Claassen,Mitchell P Levesque,Reinhard Dummer,Sabine WernerPubMed 36914141
- Physiological research 71:379-3882022Pharmacological inhibition of eIF2alpha phosphorylation by integrated stress response inhibitor (ISRIB) ameliorates vascular calcification in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJ Dong,S Jin,J Guo,R Yang,D Tian,H Xue,L Xiao,Q Guo,R Wang,M Xu,X Teng,Y WuPubMed 35616039
- Journal of orthopaedic translation 31:52-612021NF-κB activation impedes the transdifferentiation of hypertrophic chondrocytes at the growth plate of mouse embryos in diabetic pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXi Liu,Fan Qian,Qiwei Fan,Li Lin,Meiyao He,Peizhi Li,Hongmei Cai,Lisha Ma,Xin Cheng,Xuesong YangPubMed 34934622
- International journal of nanomedicine 16:6693-67182021Mussel-Inspired Gold Nanoparticle and PLGA/L-Lysine-g-Graphene Oxide Composite Scaffolds for Bone Defect Repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan Fu,Yikun Jiang,Xiaoyu Yang,Yu Wang,Wei Ji,Guoliang JiaPubMed 34621123
- Experimental and therapeutic medicine 22:9402021Psoralen accelerates osteogenic differentiation of human bone marrow mesenchymal stem cells by activating the TGF-β/Smad3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongquan Huang,Liu Liao,Haitao Su,Xinlin Chen,Tao Jiang,Jun Liu,Qiuke HouPubMed 34306204
- Experimental and therapeutic medicine 21:1972021Shuxuetong injection simultaneously ameliorates dexamethasone-driven vascular calcification and osteoporosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhe Xu et. al.PubMed 33488806
- Journal of controlled release : official journal of the Controlled Release Society 329:270-2852020Fabricating a novel HLC-hBMP2 fusion protein for the treatment of bone defects.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuoyue Chen,Zhen Zhang,Zhaoyue Wang,Jiawei Wu,Yihang Wang,He Si,Xin Xie,Lijun Shang,Daidi Fan,Fulin ChenPubMed 33278483
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| ICC | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed:28505335, PubMed:28703881, PubMed:28738062). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
Alternative names
AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A
Recommended products
Rabbit Polyclonal RUNX2 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 15 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RUNX2 also known as core-binding factor subunit alpha-1 (CBFA1) is a transcription factor with a molecular weight of approximately 56 kDa. It plays a pivotal role in regulating osteoblast differentiation and skeletal morphogenesis. This protein resides mainly in the nucleus and shows a high expression in bone tissue. RUNX2 binds to DNA sequences to control transcription of genes essential for bone development and maturation. The protein also possesses a Runt-homology domain enabling it to attach to specific DNA sequences.
- Biological function summary
This protein orchestrates bone formation by influencing the expression of genes involved in osteoblast differentiation. RUNX2 operates as part of the larger transcriptional complex that includes other proteins that modulate its activity. It maintains bone homeostasis by regulating the expression of osteogenic markers including bone sialoprotein and osteocalcin. The ability of RUNX2 to drive osteoblast lineage commitment highlights its importance in skeletal health and development.
- Pathways
RUNX2 shows significant involvement in both the Wnt signaling and TGF-beta signaling pathways. Within the Wnt signaling pathway RUNX2 interacts with beta-catenin to facilitate osteoblastogenesis and support bone matrix deposition. In the TGF-beta signaling pathway RUNX2 modulates Smad-dependent transcriptional activity important for extracellular matrix production and bone remodeling. These interactions illustrate RUNX2's central role in mediating bone development and maintenance.
- Associated diseases and disorders
RUNX2 mutations and dysregulation link to cleidocranial dysplasia a condition characterized by delayed closure of sutures in the skull and other skeletal anomalies. Additionally RUNX2 is related to osteosarcoma a type of bone cancer where its abnormal expression often contributes to tumor progression. In these contexts RUNX2 interacts with proteins like p53 an important regulator of cell cycle highlighting its influence on tumor suppressor networks and osteogenic pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry - Anti-RUNX2 antibody (ab114133)
ab114133 staining RUNX2 in U2OS cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab114133 at 5µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
Western blot - Anti-RUNX2 antibody (ab114133)
The predicted molecular weight of RUNX2 is 56 kDa (SwissProt), however we expect to observe a banding pattern around 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This antibody was raised against an immunogen that is predicted to recognize isoforms 1,2 and 3 of RUNX2. The predicted molecular weights of isoforms 1,2 and 3 are 56, 55 and 54 kDa respectively.All lanes: Western blot - Anti-RUNX2 antibody (ab114133) at 1 µg/mL
All lanes: Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 64 kDa, 65 kDa
Exposure time: 1min
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