JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275219

Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (Capture)

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal RUNX2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

AML3, CBFA1, OSF2, PEBP2A, RUNX2, Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, Polyomavirus enhancer-binding protein 2 alpha A subunit, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, CBF-alpha-1, OSF-2, PEA2-alpha A, PEBP2-alpha A

1 Images
Sandwich ELISA - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (Capture) (AB275219)
  • sELISA

Lab

Sandwich ELISA - Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free (Capture) (AB275219)

Sandwich ELISA of ab253543 with the capture antibody ab275219 dilution at 2 µg/mL and detector antibody ab275220 dilution at 0.5 µg/mL.

  • 578 PE

    PE Anti-RUNX2 antibody [EPR22858-106] – ChIP Grade

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-RUNX2 antibody [EPR22858-106] – ChIP Grade

  • 660 APC

    APC Anti-RUNX2 antibody [EPR22858-106] – ChIP Grade

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-RUNX2 antibody [EPR22858-106] – ChIP Grade

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-RUNX2 antibody [EPR22858-106] – ChIP Grade

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-RUNX2 antibody [EPR22858-106] – ChIP Grade

  • Unconjugated

    Anti-RUNX2 antibody [EPR22858-106] - ChIP Grade

  • Carrier free

    Anti-RUNX2 antibody [EPR22858-106] - BSA and Azide free

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-RUNX2 antibody [EPR22858-106] - ChIP Grade

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22858-106

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
style
left-column

Product details

ab275219 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human RUNX2. The recommended pair for sandwich ELISA is:
Capture: ab275219, Human RUNX2 Capture Antibody (unconjugated)
Detector: ab275220, Human RUNX2 Detector Antibody (unconjugated)
The reference range value is 15.63 - 2000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RUNX2 also known as core-binding factor subunit alpha-1 (CBFA1) is a transcription factor with a molecular weight of approximately 56 kDa. It plays a pivotal role in regulating osteoblast differentiation and skeletal morphogenesis. This protein resides mainly in the nucleus and shows a high expression in bone tissue. RUNX2 binds to DNA sequences to control transcription of genes essential for bone development and maturation. The protein also possesses a Runt-homology domain enabling it to attach to specific DNA sequences.
Biological function summary

This protein orchestrates bone formation by influencing the expression of genes involved in osteoblast differentiation. RUNX2 operates as part of the larger transcriptional complex that includes other proteins that modulate its activity. It maintains bone homeostasis by regulating the expression of osteogenic markers including bone sialoprotein and osteocalcin. The ability of RUNX2 to drive osteoblast lineage commitment highlights its importance in skeletal health and development.

Pathways

RUNX2 shows significant involvement in both the Wnt signaling and TGF-beta signaling pathways. Within the Wnt signaling pathway RUNX2 interacts with beta-catenin to facilitate osteoblastogenesis and support bone matrix deposition. In the TGF-beta signaling pathway RUNX2 modulates Smad-dependent transcriptional activity important for extracellular matrix production and bone remodeling. These interactions illustrate RUNX2's central role in mediating bone development and maintenance.

RUNX2 mutations and dysregulation link to cleidocranial dysplasia a condition characterized by delayed closure of sutures in the skull and other skeletal anomalies. Additionally RUNX2 is related to osteosarcoma a type of bone cancer where its abnormal expression often contributes to tumor progression. In these contexts RUNX2 interacts with proteins like p53 an important regulator of cell cycle highlighting its influence on tumor suppressor networks and osteogenic pathways.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis (PubMed : 28505335, PubMed : 28703881, PubMed : 28738062). Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. In osteoblasts, supports transcription activation : synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE) (By similarity). Inhibits KAT6B-dependent transcriptional activation.
See full target information RUNX2
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB100632

Human RAGE ELISA Kit

Sandwich ELISA - Human RAGE ELISA Kit (AB100632)

  • 3
  • 1
Primary Antibodies

AB272276

Anti-Osteocalcin antibody [EPR22338-92] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Osteocalcin antibody [EPR22338-92] - BSA and Azide free (Detector) (AB272276)

  • 4
  • 1
Primary Antibodies

AB22552

Anti-Sp7 / Osterix antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sp7 / Osterix antibody (AB22552)

  • 5
  • 24
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB214821

Anti-BMP2 antibody [EPR20807]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-BMP2 antibody [EPR20807] (AB214821)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com