Skip to main content

Mouse Monoclonal RUNX3 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RUNX3 aa 150-300.


Images

Flow Cytometry - Anti-RUNX3 antibody [2B3] (AB135248), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [2B3] (AB135248), expandable thumbnail
  • Western blot - Anti-RUNX3 antibody [2B3] (AB135248), expandable thumbnail
  • Western blot - Anti-RUNX3 antibody [2B3] (AB135248), expandable thumbnail
  • Western blot - Anti-RUNX3 antibody [2B3] (AB135248), expandable thumbnail

Publications

Key facts

Isotype

IgG2b

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human RUNX3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13761

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBIHC-PICC/IF
Human
Expected
Tested
Tested
Expected
Mouse
Tested
Expected
Expected
Expected

Tested
Tested

Species

Mouse

Dilution info
1/200.00000 - 1/400.00000
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Expected
Expected

Species

Mouse, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Forms the heterodimeric complex core-binding factor (CBF) with CBFB. RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (By similarity). May be involved in the control of cellular proliferation and/or differentiation. In association with ZFHX3, up-regulates CDKN1A promoter activity following TGF-beta stimulation (PubMed:20599712). CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding sequence within the ZBTB7B locus acting as transcriptional silencer and allowing for cytotoxic T cell differentiation. CBF complexes binding to the transcriptional silencer is essential for recruitment of nuclear protein complexes that catalyze epigenetic modifications to establish epigenetic ZBTB7B silencing (By similarity).

Alternative names

AML2, CBFA3, PEBP2A3, RUNX3, Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, Core-binding factor subunit alpha-3, Oncogene AML-2, Polyomavirus enhancer-binding protein 2 alpha C subunit, SL3-3 enhancer factor 1 alpha C subunit, SL3/AKV core-binding factor alpha C subunit, CBF-alpha-3, PEA2-alpha C, PEBP2-alpha C

Recommended products

Mouse Monoclonal RUNX3 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human RUNX3 aa 150-300.

Key facts

Isotype

IgG2b

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human RUNX3 aa 150-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13761
Clone number

2B3

Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Notes

This product was changed from ascites to supernatant. Lot no's high than GR206339-20 are from Tissue Culture Supernatant

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

RUNX3 also known as Runt-related transcription factor 3 is a protein that plays an important role in regulating gene expression. The protein has a mass of approximately 44 kDa. RUNX3 is found mainly in tissues like the gastrointestinal tract and neural systems. It functions as a DNA-binding transcription factor that regulates various genes responsible for important cellular processes.

Biological function summary

The function of RUNX3 involves the regulation of cell proliferation differentiation and apoptosis. RUNX3 often forms a complex with the core-binding factor beta (CBFβ) enhancing its stability and DNA-binding capability. This protein is essential in maintaining cellular homeostasis and influencing the immune response particularly through its role in T cell development.

Pathways

RUNX3 is heavily involved in the TGF-beta signaling pathway and the Wnt signaling pathway. It interacts with several proteins such as SMADs in the TGF-beta pathway to modulate gene expression that affects cell growth and survival. Interaction with beta-catenin in the Wnt signaling pathway further emphasizes its importance in regulating cell fate decisions.

Associated diseases and disorders

Alterations in RUNX3 expression or function are linked to cancer including gastric cancer and colorectal cancer. It often shows reduced activity or expression in these cancers which associates with proteins like TGF-beta leading to impaired cell growth control. RUNX3 dysregulation is also connected to neurological disorders due to its role in neural development and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry - Anti-RUNX3 antibody [2B3] (ab135248), expandable thumbnail

    Flow Cytometry - Anti-RUNX3 antibody [2B3] (ab135248)

    Flow cytometric analysis of NIH 3T3 cells labelling RUNX3 with ab135248 at 1/200 dilution (green). Negative control (red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [2B3] (ab135248), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RUNX3 antibody [2B3] (ab135248)

    Immunohistochemical analysis of Paraffin-embedded Human cervical cancer tissue labelling RUNX3 with ab135248 at 1/200 dilution followed by DAB staining.

  • Western blot - Anti-RUNX3 antibody [2B3] (ab135248), expandable thumbnail

    Western blot - Anti-RUNX3 antibody [2B3] (ab135248)

    Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
    Lane 2: RUNX3 knockout HAP1 whole cell lysate (20 μg)
    Lane 3: HeLa whole cell lysate (20 μg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab135248 observed at 44 kDa. Red - loading control, Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560, observed at 50 kDa.

    ab135248 was shown to recognize RUNX3 in wild-type HAP1 cells as signal was lost at the expected MW in RUNX3 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and RUNX3 knockout samples were subjected to SDS-PAGE. ab135248 and Anti-alpha Tubulin antibody [EPR13478(B)] - Loading Control ab176560 (Rabbit anti-alpha Tubulin loading control) were incubated overnight at 4°C at 1/500 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-RUNX3 antibody [2B3] (ab135248)

    Predicted band size: 44 kDa

  • Western blot - Anti-RUNX3 antibody [2B3] (ab135248), expandable thumbnail

    Western blot - Anti-RUNX3 antibody [2B3] (ab135248)

    All lanes: Western blot - Anti-RUNX3 antibody [2B3] (ab135248) at 1/500 dilution

    All lanes: Recombinant Human RUNX3 protein

    Predicted band size: 44 kDa

  • Western blot - Anti-RUNX3 antibody [2B3] (ab135248), expandable thumbnail

    Western blot - Anti-RUNX3 antibody [2B3] (ab135248)

    All lanes: Western blot - Anti-RUNX3 antibody [2B3] (ab135248) at 1/500 dilution

    Lane 1: HEK293 cell lysate, non-transfected

    Lane 2: HEK293 cell lysate, transfected with RUNX3 (amino acids 186-252)-hIgGFc

    Predicted band size: 44 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com