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AB181994

Anti-RWDD1 antibody [EPR13715(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal RWDD1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

DFRP2, CGI-24, PTD013, RWDD1, RWD domain-containing protein 1, DRG family-regulatory protein 2

3 Images
Flow Cytometry (Intracellular) - Anti-RWDD1 antibody [EPR13715(B)] (AB181994)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-RWDD1 antibody [EPR13715(B)] (AB181994)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixedHelacells labeling RWDD1with ab181994at a 1/10 dilution, or with rabbit IgG isotype control. Goat anti rabbit IgG (FITC) at a 1/150 dilutionwas used as a secondary antibody.

Immunoprecipitation - Anti-RWDD1 antibody [EPR13715(B)] (AB181994)
  • IP

Supplier Data

Immunoprecipitation - Anti-RWDD1 antibody [EPR13715(B)] (AB181994)

Immunoprecipitation of Jurkat labeling RWDD1 using ab181994 at a 1/60 dilution (lane 1). Lane 2 shows the negative control. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as a secondary antibody at a dilution of 1/1500. Blocking/dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-RWDD1 antibody [EPR13715(B)] (ab181994)

Predicted band size: 28 kDa

false

Western blot - Anti-RWDD1 antibody [EPR13715(B)] (AB181994)
  • WB

Supplier Data

Western blot - Anti-RWDD1 antibody [EPR13715(B)] (AB181994)

All lanes:

Western blot - Anti-RWDD1 antibody [EPR13715(B)] (ab181994) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Lane 4:

A673 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 28 kDa

false

  • Carrier free

    Anti-RWDD1 antibody [EPR13715(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13715(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RWDD1 also known as RWD domain-containing protein 1 is a protein with a molecular mass of approximately 12 kDa. This protein is expressed in various tissues including the brain liver and kidney. Mechanically RWDD1 interacts with other proteins through its RWD domain which is a conserved region facilitating protein-protein interactions. This domain helps RWDD1 participate in cellular processes by serving as a regulatory subunit in protein complexes.
Biological function summary

RWDD1 influences cellular activities due to its role in modulating protein degradation pathways. It is part of protein complexes that are involved in tagging proteins for degradation. This modulation affects many cellular processes such as cell cycle control and stress responses. The protein may work closely with proteasomal subunit proteins suggesting it has a specific function in maintaining cellular protein homeostasis.

Pathways

RWDD1 is actively involved in the ubiquitin-proteasome system which is a significant pathway for protein degradation. It interacts with proteins involved in ubiquitination processes which tag proteins for breakdown. Additionally RWDD1 plays a role in cellular response pathways to oxidative stress through associations with proteins like Nrf2 a well-known regulator of the antioxidant response.

Dysregulation of RWDD1 may link to cancer and neurodegenerative diseases. Abnormal RWDD1 function can lead to impaired protein degradation contributing to the accumulation of damaged or misfolded proteins a common feature in these conditions. Associations with proteins such as p53 in cancer and links to tau proteins in Alzheimer’s disease highlight its potential involvement in disease mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protects DRG2 from proteolytic degradation.
See full target information RWDD1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ biofilms and microbiomes 11:93 PubMed40461483

2025

TSP50 deficiency in neural stem cells aggravates colitis in mice by altering intestinal microbiome.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoli Li,Rong Jin,Zhaoxia Wang,Chunxue Niu,Zhenbo Song,Xiaoling Liu,Jian Huang,Huan Zhang,Xia Qian,Feng Gao,Shuyue Wang,Chunlei Yu,Luguo Sun,Yanxin Huang,Lihua Zheng,Guannan Wang,Ying Sun,Xiaoguang Yang,Yongli Bao,Jiawei Li
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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