JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB53162

Anti-RXRG antibody

3

(3 Reviews)

|

(2 Publications)

Rabbit Polyclonal RXRG antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human RXRG.

View Alternative Names

NR2B3, RXRG, Retinoic acid receptor RXR-gamma, Nuclear receptor subfamily 2 group B member 3, Retinoid X receptor gamma

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RXRG antibody (AB53162)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RXRG antibody (AB53162)

ab53162 staining RXRG in human nucleus accumbens.
Left panel : with primary antibody at a dilution of 4μg/ml. Right panel : isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature : sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH9.0 in a Dako PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human RXRG. The exact immunogen used to generate this antibody is proprietary information.

P48443

Specificity

ab53162 detects endogenous levels of total Retinoic X Receptor gamma protein.

style
left-column

Complementary Products

Primary Antibodies

AB125001

Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Retinoid X Receptor alpha/RXRA antibody [EPR7106] (AB125001)

  • 5
  • 5
Primary Antibodies

AB31940

Anti-TBR1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TBR1 antibody (AB31940)

  • 4
  • 34
Primary Antibodies

AB53161

Anti-Retinoic Acid Receptor beta antibody

Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (AB53161)

  • 0
  • 2
Primary Antibodies

AB187159

Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor gamma antibody [EPR2020(N)] - N-terminal (AB187159)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2-4 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab53162 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RXRG also known as retinoid X receptor gamma is a nuclear receptor protein weighing approximately 55 kDa. It binds retinoic acids and mediates their effects in the cell. RXRG can form homodimers or heterodimers with other nuclear receptors. The protein's expression occurs mainly in the brain muscles and the spleen. As a transcription factor it regulates gene expression by binding to specific DNA sequences influencing various cellular processes.
Biological function summary

RXRG acts as a transcriptional regulator in the retinoid signaling pathway. It forms part of a heterodimer complex with receptors such as PPARs (peroxisome proliferator-activated receptors) and RXR α and β subunits. These complexes control genes involved in cell differentiation proliferation and apoptosis. Through RXRG cells can transduce external retinoid signals into specific gene expression responses impacting development and metabolic processes.

Pathways

RXRG integrates into significant signaling cascades including the retinoic acid signaling pathway and the adipogenesis pathway. It often partners with other nuclear receptors like RARs (retinoic acid receptors) and PPARs to mediate these pathways. For instance in the adipogenesis pathway RXRG's interaction with PPARγ regulates adipocyte differentiation and lipid metabolism. These pathways link to extensive roles in development and metabolic regulation.

RXRG plays a role in conditions such as obesity and certain types of cancer. In obesity the interaction between RXRG and PPARγ influences fat cell formation and energy balance. Additionally dysregulation of RXRG expression associates with cancers where it interacts with proteins like RXRα to alter cell proliferation and apoptosis. Understanding these associations highlights RXRG's importance in therapeutic research and potential drug targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. The high affinity ligand for RXRs is 9-cis retinoic acid (By similarity).
See full target information RXRG
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroimmune pharmacology : the official 10:55-73 PubMed25586717

2015

Cocaine induces nuclear export and degradation of neuronal retinoid X receptor-γ via a TNF-α/JNK- mediated mechanism.

Applications

ICC/IF, WB

Species

Mouse, Mouse

Jane Kovalevich,William Yen,Ahmet Ozdemir,Dianne Langford

Cell 142:320-32 PubMed20655472

2010

SIRT1 suppresses beta-amyloid production by activating the alpha-secretase gene ADAM10.

Applications

WB

Species

Mouse

Gizem Donmez,Diana Wang,Dena E Cohen,Leonard Guarente
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com