AB196355

Anti-Ryanodine receptor 2/RYR-2 antibody - N-terminal

Name: Anti-Ryanodine receptor 2/RYR-2 antibody - N-terminal (ab196355) | Abcam
Brand: Abcam Limited
SKU: AB196355
Price: 535 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal Ryanodine receptor 2/RYR-2 antibody. N-terminal. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human RYR2.

View Alternative Names

Ryanodine receptor 2, RYR-2, RyR2, hRYR-2, Cardiac muscle ryanodine receptor, Cardiac muscle ryanodine receptor-calcium release channel, Type 2 ryanodine receptor, RYR2

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human RYR2. The exact immunogen used to generate this antibody is proprietary information.

Q92736

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ryanodine receptor 2 (RYR-2) also known as cardiac ryanodine receptor is an intracellular calcium release channel that plays an important role in cardiac muscle contraction. This large protein has a mass of about 564 kDa and is mainly expressed in cardiac muscle tissue. Mechanically RYR-2 regulates the release of calcium ions from the sarcoplasmic reticulum into the cytosol. This calcium release occurs through the calcium-induced calcium release mechanism which is important in excitation-contraction coupling in cardiac muscle cells.

Biological function summary

RYR-2 enables the heart muscle cells to contract and relax by supplying calcium ions necessary for muscle contraction. It is part of a larger complex that includes proteins such as FK506-binding protein 12.6 (FKBP12.6) and calsequestrin which help regulate its function. Proper function of this complex is necessary for maintaining the rhythmic contraction and relaxation cycles of the heart. Any disruptions in this function can lead to irregular heartbeats.

Pathways

RYR-2 is significant in cardiac calcium signaling pathways that involve calcium-induced calcium release. Through this pathway it works closely with L-type calcium channels like Cav1.2 which are responsible for the initial influx of calcium that activates RYR-2. This cooperation allows for the amplification of calcium signals needed for strong cardiac muscle contractions. Proper signaling in these pathways is necessary for maintenance of heart rhythm and contractile strength.

RYR-2 mutations associate with several cardiac disorders including catecholaminergic polymorphic ventricular tachycardia (CPVT) and arrhythmogenic right ventricular dysplasia type 2 (ARVD2). These conditions involve irregular heartbeats often triggered by physical exercise or stress. The relationship between RYR-2 and these disorders connects through its dysfunction in calcium signaling regulation which impacts heart rhythm stability. The malfunctioning of RYR-2 also affects related proteins like FKBP12.6 contributing further to disrupted calcium homeostasis and arrhythmic events.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering cardiac muscle contraction. Aberrant channel activation can lead to cardiac arrhythmia. In cardiac myocytes, calcium release is triggered by increased Ca(2+) cytosolic levels due to activation of the L-type calcium channel CACNA1C. The calcium channel activity is modulated by formation of heterotetramers with RYR3. Required for cellular calcium ion homeostasis. Required for embryonic heart development.

See full target information RYR2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Animal bioscience 38:303-315 PubMed39210798

2024

shRNA-interfered of Nrf2 reveals a critical role for Keap1-Nrf2 signaling pathway during effects of zearalenone induced oxidative stress in IPEC-J2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qun Cheng,Shu Zhen Jiang,Li Bo Huang,Wei Ren Yang

International journal of molecular sciences 25: PubMed39000424

2024

Generation and Accumulation of Various Advanced Glycation End-Products in Cardiomyocytes May Induce Cardiovascular Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takanobu Takata,Shinya Inoue,Togen Masauji,Katsuhito Miyazawa,Yoshiharu Motoo

Cell reports 40:111203 PubMed35977512

2022

STK25 inhibits PKA signaling by phosphorylating PRKAR1A.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaokan Zhang,Bryan Z Wang,Michael Kim,Trevor R Nash,Bohao Liu,Jenny Rao,Roberta Lock,Manuel Tamargo,Rajesh Kumar Soni,John Belov,Eric Li,Gordana Vunjak-Novakovic,Barry Fine

BMC complementary medicine and therapies 22:115 PubMed35468773

2022

Trans-cinnamaldehyde protects against phenylephrine-induced cardiomyocyte hypertrophy through the CaMKII/ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Qian,Jing Tian,Sining Wang,Xiaoli Shan,Pei Zhao,Huihua Chen,Ming Xu,Wei Guo,Chen Zhang,Rong Lu

Circulation 139:949-964 PubMed30586718

2018

Ryanodine Receptor Glycation Favors Mitochondrial Damage in the Senescent Heart.

Applications

Unspecified application

Species

Unspecified reactive species

Marisol Ruiz-Meana,Marta Minguet,Diana Bou-Teen,Elisabet Miro-Casas,Celia Castans,Jose Castellano,Elena Bonzon-Kulichenko,Alberto Igual,Rafael Rodriguez-Lecoq,Jesús Vázquez,David Garcia-Dorado

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ryanodine receptor 2/RYR-2 antibody - N-terminal

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise