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AB219798

Anti-Ryanodine Receptor antibody [EPR21796]

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(8 Publications)

Rabbit Recombinant Monoclonal Ryanodine Receptor antibody. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

View Alternative Names

RYDR, RYR1, Ryanodine receptor 1, RYR-1, RyR1, Skeletal muscle calcium release channel, Skeletal muscle ryanodine receptor, Skeletal muscle-type ryanodine receptor, Type 1 ryanodine receptor

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse skeletal muscle (PMID : 26109061) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat skeletal muscle tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive staining of rat skeletal muscle cells (PMID : 21454501) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of mouse cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of rat cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of C2C12 (mouse muscle myoblast) cells labeling Ryanodine Receptor (green) with ab219798 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Confocal image showing cytoplasmic staining in differentiated C2C12 cells. Confluent C2C12 cells were grown for 8 days to differentiate into myotube in a complete culture medium containing 10% FBS, following the ATCC protocol for myotube formation. The nuclear counter stain is DAPI (blue).

ab195889, Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Ryanodine Receptor with ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in rat skeletal muscle (PMID : 26109061) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive cytoplasmic staining in Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of rat cerebellum tissue section (PMID : 18313230) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse skeletal muscle tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive staining in mouse skeletal muscle cells (PMID : 21454501) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling Ryanodine Receptor (green) with ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. Positive cytoplasmic staining in the Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of mouse cerebellum tissue section (PMID : 18313230) is observed. The nuclear counter stain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

Western blot - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)
  • WB

Supplier Data

Western blot - Anti-Ryanodine Receptor antibody [EPR21796] (AB219798)

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure times.

Lanes 1 & 3 : 3 minutes.

Lane 2 : 15 seconds.

All lanes:

Western blot - Anti-Ryanodine Receptor antibody [EPR21796] (ab219798) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 3:

Rat skeletal muscle tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 565 kDa

Observed band size: 565 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21796

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine receptor 2/RYR-2 antibody [EPR26288-70] (AB302716)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/30", "IHCFr-species-notes": "<p>Perform heat-mediated antigen retrieval by using <a href='/en-us/products/unavailable/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab94681'>ab94681</a> (Tris/EDTA buffer, pH9.0).</p><p>IHC is not recommended in human due to non-specific staining.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p>IHC is not recommended in human due to non-specific staining.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/30", "IHCFr-species-notes": "<p>Perform heat-mediated antigen retrieval by using <a href='/en-us/products/unavailable/antigen-retrieval-buffer-100x-tris-edta-buffer-ph-90-ab94681'>ab94681</a> (Tris/EDTA buffer, pH9.0).</p><p>IHC is not recommended in human due to non-specific staining.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "<p>IHC is not recommended in human due to non-specific staining.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ryanodine receptor also known as RyR is a significant calcium ion channel that facilitates the release of calcium from the sarcoplasmic reticulum into the cytosol. It is especially important in cardiac and skeletal muscle cells influencing muscle contraction. The receptor exists in three isoforms: RyR1 RyR2 and RyR3 with RyR1 dominant in skeletal muscle RyR2 in cardiac muscle and RyR3 more ubiquitously in the body. RyR is a large protein with a mass of approximately 2 megadaltons comprising multiple subunits and forming part of a transmembrane complex.
Biological function summary

These receptors regulate calcium ions important for triggering muscle contractions and other calcium-dependent processes. RyR operates as part of a complex with other proteins such as FKBP12 (calstabin) and calmodulin which modulate its function. The receptor plays an important role in excitation-contraction coupling in muscles ensuring proper physiological responses to stimuli. Calcium release through these channels not only manages contraction but also assists in signal transduction within various cellular contexts.

Pathways

The ryanodine receptor integrates significantly into the excitation-contraction coupling and the calcium signaling pathways. In excitation-contraction coupling the receptor responds to electrical stimuli by releasing calcium that initiates contraction. In the calcium signaling pathway it participates by releasing and regulating intracellular calcium level interacting with proteins such as L-type calcium channels and calsequestrin to maintain cell homeostasis. These interactions define the RyR's role in cellular respiration muscle contraction and other physiological processes.

Abnormal function or mutations in RyR often associate with cardiac and skeletal muscle diseases such as malignant hyperthermia and catecholaminergic polymorphic ventricular tachycardia. Dysregulation or mutation-induced alterations affect calcium release and contribute to pathogenesis. Connections between RyR dysfunction and other proteins such as junctin or triadin in muscle cells frequently disrupt normal muscle function illustrating RyR's significant role in these disorders. The receptor's involvement in calcium dysregulation suggests potential avenues for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules (PubMed : 11741831, PubMed : 16163667, PubMed : 18268335, PubMed : 18650434, PubMed : 26115329). Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm (PubMed : 18268335). Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis (By similarity).
See full target information RYR1
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1557685 PubMed40206075

2025

Allicin ameliorates acute myocardial infarction in rats by modulating calcium homeostasis in cardiomyocytes through the induction of hydrogen sulfide production.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyu Liu,Shaojun Xu,Juan Wang,Xinxia Li,Ruiting Liu,Le Zhao,Yikui Li,Rongmei Shi,Jinyan Zhang

Frontiers in cell and developmental biology 11:1243299 PubMed37745298

2023

Shank3 related muscular hypotonia is accompanied by increased intracellular calcium concentrations and ion channel dysregulation in striated muscle tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Berra Yildiz,Lisa Schiedt,Medhanie Mulaw,Jürgen Bockmann,Sarah Jesse,Anne-Kathrin Lutz,Tobias M Boeckers

ACS applied bio materials 6:2860-2874 PubMed37342003

2023

Conductive Bacterial Nanocellulose-Polypyrrole Patches Promote Cardiomyocyte Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Sumithra Yasaswini Srinivasan,Marina Cler,Osnat Zapata-Arteaga,Bernhard Dörling,Mariano Campoy-Quiles,Elena Martínez,Elisabeth Engel,Soledad Pérez-Amodio,Anna Laromaine

Biomedicines 11: PubMed37189811

2023

Age-Dependent Changes in Calcium Regulation after Myocardial Ischemia-Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Bencurova,Terezia Lysikova,Katarina Leskova Majdova,Peter Kaplan,Peter Racay,Jan Lehotsky,Zuzana Tatarkova

iScience 25:104577 PubMed35789849

2022

Microgravity-induced stress mechanisms in human stem cell-derived cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Aviseka Acharya,Harshal Nemade,Symeon Papadopoulos,Jürgen Hescheler,Felix Neumaier,Toni Schneider,Krishna Rajendra Prasad,Khadija Khan,Ruth Hemmersbach,Eduardo Gade Gusmao,Athanasia Mizi,Argyris Papantonis,Agapios Sachinidis

Physiological reports 9:e15011 PubMed34523260

2021

The ryanodine receptor stabilizer S107 ameliorates contractility of adult Rbm20 knockout rat cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Chaoqun Zhu,Zhiyong Yin,Yanghai Zhang,Chunyan Wang,Andrea Sanchez Walk,Ying-Hsi Lin,Timothy A McKinsey,Kathleen C Woulfe,Jun Ren,Herbert G Chew

Nature communications 12:1031 PubMed33589620

2021

Janus 3D printed dynamic scaffolds for nanovibration-driven bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Camarero-Espinosa,Lorenzo Moroni

Cancer management and research 12:137-144 PubMed32021430

2020

Desmoplastic Reaction and Tumor Budding in Cervical Squamous Cell Carcinoma are Prognostic Factors for Distant Metastasis: A Retrospective Study.

Applications

Unspecified application

Species

Unspecified reactive species

Lanqing Cao,Ping-Li Sun,Yangyang He,Min Yao,Hongwen Gao
View all publications
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Product promise

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