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Rabbit Recombinant Monoclonal Ryanodine Receptor antibody. Carrier free. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (AB231086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (AB231086), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (AB231086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (AB231086), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (AB231086), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-FrIHC-P
Human
Expected
Predicted
Predicted
Predicted
Mouse
Expected
Tested
Expected
Tested
Rat
Expected
Expected
Tested
Expected

Expected
Expected

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

IHC is not recommended in human due to non-specific staining.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

IHC is not recommended in human due to non-specific staining.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Cytosolic calcium-activated calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytosol and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules (PubMed:11741831, PubMed:16163667, PubMed:18268335, PubMed:18650434, PubMed:26115329). Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm (PubMed:18268335). Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis (By similarity).

Alternative names

RYDR, RYR1, Ryanodine receptor 1, RYR-1, RyR1, Skeletal muscle calcium release channel, Skeletal muscle ryanodine receptor, Skeletal muscle-type ryanodine receptor, Type 1 ryanodine receptor

Recommended products

Rabbit Recombinant Monoclonal Ryanodine Receptor antibody. Carrier free. Suitable for WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR21796
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab231086 is the carrier-free version of Anti-Ryanodine Receptor antibody [EPR21796] ab219798.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ryanodine receptor also known as RyR is a significant calcium ion channel that facilitates the release of calcium from the sarcoplasmic reticulum into the cytosol. It is especially important in cardiac and skeletal muscle cells influencing muscle contraction. The receptor exists in three isoforms: RyR1 RyR2 and RyR3 with RyR1 dominant in skeletal muscle RyR2 in cardiac muscle and RyR3 more ubiquitously in the body. RyR is a large protein with a mass of approximately 2 megadaltons comprising multiple subunits and forming part of a transmembrane complex.

Biological function summary

These receptors regulate calcium ions important for triggering muscle contractions and other calcium-dependent processes. RyR operates as part of a complex with other proteins such as FKBP12 (calstabin) and calmodulin which modulate its function. The receptor plays an important role in excitation-contraction coupling in muscles ensuring proper physiological responses to stimuli. Calcium release through these channels not only manages contraction but also assists in signal transduction within various cellular contexts.

Pathways

The ryanodine receptor integrates significantly into the excitation-contraction coupling and the calcium signaling pathways. In excitation-contraction coupling the receptor responds to electrical stimuli by releasing calcium that initiates contraction. In the calcium signaling pathway it participates by releasing and regulating intracellular calcium level interacting with proteins such as L-type calcium channels and calsequestrin to maintain cell homeostasis. These interactions define the RyR's role in cellular respiration muscle contraction and other physiological processes.

Associated diseases and disorders

Abnormal function or mutations in RyR often associate with cardiac and skeletal muscle diseases such as malignant hyperthermia and catecholaminergic polymorphic ventricular tachycardia. Dysregulation or mutation-induced alterations affect calcium release and contribute to pathogenesis. Connections between RyR dysfunction and other proteins such as junctin or triadin in muscle cells frequently disrupt normal muscle function illustrating RyR's significant role in these disorders. The receptor's involvement in calcium dysregulation suggests potential avenues for therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of C2C12 (mouse muscle myoblast) cells labeling Ryanodine Receptor (green) with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% TritonX-100. Confocal image showing cytoplasmic staining in differentiated C2C12 cells. Confluent C2C12 cells were grown for 8 days to differentiate into myotube in a complete culture medium containing 10% FBS, following the ATCC protocol for myotube formation. The nuclear counter stain is DAPI (blue).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 was used as counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling Ryanodine Receptor with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of mouse cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse skeletal muscle tissue labeling Ryanodine Receptor (green) with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive staining in mouse skeletal muscle cells (PMID: 21454501) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling Ryanodine Receptor with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in Purkinje cells of rat cerebellum (PMID 18313230) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Ryanodine Receptor with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in rat skeletal muscle (PMID: 26109061) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Ryanodine Receptor with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in mouse skeletal muscle (PMID: 26109061) is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat cerebellum tissue labeling Ryanodine Receptor (green) with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive cytoplasmic staining in Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of rat cerebellum tissue section (PMID: 18313230) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned rat skeletal muscle tissue labeling Ryanodine Receptor (green) with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive staining of rat skeletal muscle cells (PMID: 21454501) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

  • Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Ryanodine Receptor antibody [EPR21796] - BSA and Azide free (ab231086)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen sectioned mouse cerebellum tissue labeling Ryanodine Receptor (green) with Anti-Ryanodine Receptor antibody [EPR21796] ab219798 at 1/30 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Positive cytoplasmic staining in the Purkinjie cells, negative staining in cells localized in granular layer and molecular layer of mouse cerebellum tissue section (PMID: 18313230) is observed. The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

    Perform heat-mediated antigen retrieval by using ab94681 (Tris/EDTA buffer, pH9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ryanodine Receptor antibody [EPR21796] ab219798).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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