Anti-RYBP antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (AB5976)

Immunofluorescence of DEDAF in A549 cells using ab5976 at 20 ug/ml.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RYBP antibody (AB5976)

Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 μg/ml.

• ICC/IF

AbReview26126****

Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (AB5976)

ab5976 staining RYBP in Mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.5% and blocked with 1% milk for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 22°C. An Alexa Fluor^®488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/400) was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Dr. Ligia Tavares

• WB

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Western blot - Anti-RYBP antibody (AB5976)

Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 μg /ml.

Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.

All lanes:

Western blot - Anti-RYBP antibody (ab5976)

Predicted band size: 25 kDa

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• WB

CiteAb

Western blot - Anti-RYBP antibody (AB5976)

RYBP western blot using anti-RYBP antibody ab5976. Publication image and figure legend from Bian, F., Gao, F., et al., 2016, Sci Rep, PubMed 27181215.

ab5976 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab5976 please see the product overview.

Analysis of uterine expression for Cbx2, Cbx4, Ring1B, and Rybp during early pregnancy at protein levels.(A) Western blot analysis. Total protein extracts of uterine tissues were collected during early pregnancy on D4, D5-IS, D7-IIS, and D7-IS. Experimentally induced deciduoma and control horns were also analyzed on D7 pseudopregnancy. Ut, uterus; IS, implantation site; IIS, interimplantation site; Co, control; DM, deciduoma. (B) Quantitation was achieved by direct analysis of the bands from (A). Fold changes in protein levels were normalized by Actin. *Values are statistically different (p < 0.05). (C) IHC localization of Cbx2, Cbx4, and Rybp expression. Representative uterine tissue sections on D4, D5-IS, and D7-IS in the M- and AM-pole locations are shown. Sections are shown at 100X and 400X (as insets). Red staining indicates the localization of immunoreaction. le, luminal epithelium; ge, glandular epithelium, s, stroma; ds, decidualizing stroma; pdz, primary decidual zone; sdz, secondary decidual zone; dec, decidual cells; e, embryo; tgc, trophoblast giant cells; M, mesometrial pole; AM, antimesometrial pole. Arrow indicates polyploid cells. (D) IF analysis of Ring1B. Blue and green staining indicates the localization of DAPI (for nuclear) and Ring1B, respectively. Sections are shown at 100X and 400X (as insets). These experiments were repeated at least three times with similar results.

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