Rabbit Polyclonal RYBP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human RYBP aa 200 to C-terminus.
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- Journal of neuroinflammation 16:62019Early treatment with minocycline following stroke in rats improves functional recovery and differentially modifies responses of peri-infarct microglia and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWai Ping Yew et. al.PubMed 30626393
- Cellular physiology and biochemistry : internation 51:2324-23402018Circular RNA circPVT1 Promotes Proliferation and Invasion Through Sponging miR-125b and Activating E2F2 Signaling in Non-Small Cell Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiuyuan Li et. al.PubMed 30537738
- Cell stem cell 20:462-477.e62017RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanhuan Li et. al.PubMed 28111200
- Scientific reports 6:260612016Polycomb repressive complex 1 controls uterine decidualization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFenghua Bian et. al.PubMed 27181215
- Epigenetics 11:389-972016Characterization of non-canonical Polycomb Repressive Complex 1 subunits during early mouse embryogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndré Eid,Maria-Elena Torres-PadillaPubMed 27081692
- Genes & development 29:1224-382015The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilipe V Jacinto et. al.PubMed 26080816
- PLoS pathogens 9:e10038132013Biphasic euchromatin-to-heterochromatin transition on the KSHV genome following de novo infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZsolt Toth et. al.PubMed 24367262
- Nature cell biology 15:373-842013Kdm2b maintains murine embryonic stem cell status by recruiting PRC1 complex to CpG islands of developmental genes.
- Molecular cancer research : MCR 10:558-692012The unliganded glucocorticoid receptor positively regulates the tumor suppressor gene BRCA1 through GABP beta.Applications:ChIPReactive species:Unspecified reactive speciesHeather D Ritter et. al.PubMed 22328717
- Cell 148:664-782012RYBP-PRC1 complexes mediate H2A ubiquitylation at polycomb target sites independently of PRC2 and H3K27me3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human RYBP aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N488
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-1 µg/mL | Notes Can be blocked with DEDAF peptide (human). |
Species Human | Dilution info 0.5-1 µg/mL | Notes Can be blocked with DEDAF peptide (human). |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5-10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911).
Alternative names
DEDAF, YEAF1, RYBP, RING1 and YY1-binding protein, Apoptin-associating protein 1, Death effector domain-associated factor, YY1 and E4TF1-associated factor 1, APAP-1, DED-associated factor
Recommended products
Rabbit Polyclonal RYBP antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human RYBP aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human RYBP aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8N488
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
DEDAF Antibody is affinity chromatography purified via peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase 8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase 8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RYBP also known as Ring1 and YY1 Binding Protein has a critical mechanical role in gene regulation. It has a molecular weight of approximately 28 kDa. It is a part of the Polycomb group (PcG) proteins which take part in chromatin remodeling to regulate gene expression. RYBP is found in the nucleus of cells and is known to bind DNA directly. Its expression occurs widely across various tissues indicating its broad involvement in cellular functions.
- Biological function summary
This protein actively participates in maintaining transcriptional repression through its involvement in the Polycomb Repressive Complex 1 (PRC1). PRC1 includes other key proteins such as Ring1A/B and CBX proteins which work together to modulate chromatin structure. RYBP contributes to the ubiquitination of histone H2A a process essential for maintaining gene silencing. This activity is important during developmental stages influencing cell fate decisions and lineage commitment.
- Pathways
RYBP plays an essential role in the regulation of the Wnt signaling pathway and the apoptosis pathway. Within the Wnt pathway RYBP acts in part by interacting with proteins such as β-catenin influencing cell proliferation and differentiation. In the apoptosis pathway it is involved through its interactions with E3 ubiquitin ligases potentially affecting cell death regulation. These pathways showcase how RYBP integrates into important cellular processes impacting cellular homeostasis.
- Associated diseases and disorders
RYBP has connections to cancer and neurodevelopmental disorders. Its altered expression is linked to tumor progression where it interacts with other PcG proteins like EZH2 often showing overexpression in cancers. These interactions can lead to abnormal gene silencing contributing to oncogenesis. In neurodevelopmental contexts disruptions in RYBP's function are associated with changes in neural differentiation and might correlate with disorders like autism spectrum disorders highlighting its broader relevance beyond typical gene expression roles.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
This image is courtesy of a customer review submitted by Dr. Ligia TavaresImmunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976)
ab5976 staining RYBP in Mouse embryonic stem cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton 0.5% and blocked with 1% milk for 30 minutes at 22°C. Samples were incubated with primary antibody (1/1000) for 1 hour at 22°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG (H+L) polyclonal (1/400) was used as the secondary antibody.
Western blot - Anti-RYBP antibody (ab5976)
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 μg /ml.
Western blot analysis of DEDAF expression in human A549 (lane A), HepG2 (lane B), and mouse 3T3 (lane C) cell lysates withab5976 at 1 µg /ml.
All lanes: Western blot - Anti-RYBP antibody (ab5976)
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RYBP antibody (ab5976)
Immunohistochemistry of DEDAF in mouse liver tissue with DEDAF antibody at 5 μg/ml.
Immunocytochemistry/ Immunofluorescence - Anti-RYBP antibody (ab5976)
Immunofluorescence of DEDAF in A549 cells using ab5976 at 20 ug/ml.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-RYBP antibody (ab5976)
RYBP western blot using anti-RYBP antibody ab5976. Publication image and figure legend from Bian, F., Gao, F., et al., 2016, Sci Rep, PubMed 27181215.
ab5976 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab5976 please see the product overview.
Analysis of uterine expression for Cbx2, Cbx4, Ring1B, and Rybp during early pregnancy at protein levels.(A) Western blot analysis. Total protein extracts of uterine tissues were collected during early pregnancy on D4, D5-IS, D7-IIS, and D7-IS. Experimentally induced deciduoma and control horns were also analyzed on D7 pseudopregnancy. Ut, uterus; IS, implantation site; IIS, interimplantation site; Co, control; DM, deciduoma. (B) Quantitation was achieved by direct analysis of the bands from (A). Fold changes in protein levels were normalized by Actin. *Values are statistically different (P < 0.05). (C) IHC localization of Cbx2, Cbx4, and Rybp expression. Representative uterine tissue sections on D4, D5-IS, and D7-IS in the M- and AM-pole locations are shown. Sections are shown at 100X and 400X (as insets). Red staining indicates the localization of immunoreaction. le, luminal epithelium; ge, glandular epithelium, s, stroma; ds, decidualizing stroma; pdz, primary decidual zone; sdz, secondary decidual zone; dec, decidual cells; e, embryo; tgc, trophoblast giant cells; M, mesometrial pole; AM, antimesometrial pole. Arrow indicates polyploid cells. (D) IF analysis of Ring1B. Blue and green staining indicates the localization of DAPI (for nuclear) and Ring1B, respectively. Sections are shown at 100X and 400X (as insets). These experiments were repeated at least three times with similar results.
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