Rabbit Polyclonal S tag antibody. Suitable for IP, WB and reacts with Tag samples. Cited in 4 publications.
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Publications
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- The EMBO journal 41:e1107772022Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNorbert Volkmar,Christian M Gawden-Bone,James C Williamson,Jonathon Nixon-Abell,James A West,Peter H St George-Hyslop,Arthur Kaser,Paul J LehnerPubMed 35993436
- Parasites & vectors 13:4152020Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamira Salari et. al.PubMed 32787908
- The Plant cell 32:2823-28412020A SnRK1-RFWD3-Opaque2 Signaling Axis Regulates Diurnal Nitrogen Accumulation in Maize Seeds.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaobin Li et. al.PubMed 32699171
- Autophagy 15:1234-12572019Histone acetyltransferase MoHat1 acetylates autophagy-related proteins MoAtg3 and MoAtg9 to orchestrate functional appressorium formation and pathogenicity in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiyi Yin et. al.PubMed 30776962
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Tag | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes Use 2.5μg. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
Associated Products
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5 products for Alternative Product
Target data
Alternative names
KETAAAKFERQHMDS tag antibody, S peptide, S peptide epitope tag
Recommended products
Rabbit Polyclonal S tag antibody. Suitable for IP, WB and reacts with Tag samples. Cited in 4 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.
- Biological function summary
S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.
- Pathways
S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.
- Associated diseases and disorders
S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-S tag antibody (ab183674)
12% SDS-PAGE
All lanes: Western blot - Anti-S tag antibody (ab183674) at 1/5000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: S tag transfected 293T whole cell lysate at 30 µg
Predicted band size: 18 kDa
Immunoprecipitation - Anti-S tag antibody (ab183674)
S tag antibody ab183674 immunoprecipitates S tag protein in IP experiments. IP Sample: 650 μg whole cell lysate of S tag protein expressing HeLa cells (1), 20 μg whole cell lysate of transfected HeLa cells (2), Control with 2.5 μg of preimmune rabbit IgG (3). Immunoprecipitation of S tag protein by 2.5 μg of ab183674. 12% SDS-PAGE. The immunoprecipitated S tag protein was detected by ab183674 diluted at 1/10000. Anti-rabbit IgG was used as a secondary reagent.
All lanes: Immunoprecipitation - Anti-S tag antibody (ab183674)
Predicted band size: 18 kDa
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