AB183674

Anti-S tag antibody

Lab Essentials
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(9 Publications)

Rabbit Polyclonal S tag antibody. Suitable for IP, WB and reacts with Tag samples. Cited in 9 publications.

View Alternative Names

KETAAAKFERQHMDS tag antibody, S peptide, S peptide epitope tag

2 Images

Supplier Data

Immunoprecipitation - Anti-S tag antibody (AB183674)

S tag antibody ab183674 immunoprecipitates S tag protein in IP experiments. IP Sample : 650 μg whole cell lysate of S tag protein expressing HeLa cells (1), 20 μg whole cell lysate of transfected HeLa cells (2), Control with 2.5 μg of preimmune rabbit IgG (3). Immunoprecipitation of S tag protein by 2.5 μg of ab183674. 12% SDS-PAGE. The immunoprecipitated S tag protein was detected by ab183674 diluted at 1/10000. Anti-rabbit IgG was used as a secondary reagent.

All lanes:

Immunoprecipitation - Anti-S tag antibody (ab183674)

Predicted band size: 18 kDa

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Supplier Data

Western blot - Anti-S tag antibody (AB183674)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-S tag antibody (ab183674) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

S tag transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.5 µg", "IP-species-notes": "<p>Use 2.5μg.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.

Biological function summary

S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.

Pathways

S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.

S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information S tag

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 6:102319 PubMed40882623

2025

Selenized neural stem cell-derived exosomes: A neotype therapeutic agent for traumatic injuries of the central nervous system.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Wang,Guihong Lu,Peilin Guo,Haochong Zhang,Yan Wang,Diwei Zheng,Chengliang Lyu,Dongfang Wang,Shang Li,Feng Li,Jiawei Zhao,Meng Qin,Weiping Li,Hui Tan,Guanghui Ma,Wei Wei

Frontiers in cellular and infection microbiology 15:1631926 PubMed40799935

2025

Characterization of key spike RBD residues influencing SARS-CoV-2 variant adaptation to avian ACE2.

Applications

Unspecified application

Species

Unspecified reactive species

Weitong Yao,Yujun Li,Huize Sun,Danting Ma,Xiaojuan Tang,Aiping Zeng,Fang Huang

Microbiology spectrum 11:e0267623 PubMed37943512

2023

Evolution of SARS-CoV-2 Spikes shapes their binding affinities to animal ACE2 orthologs.

Applications

Unspecified application

Species

Unspecified reactive species

Weitong Yao,Yujun Li,Danting Ma,Xudong Hou,Haimin Wang,Xiaojuan Tang,Dechun Cheng,He Zhang,Chengzhi Du,Hong Pan,Chao Li,Hua Lin,Mengsi Sun,Qiang Ding,Yingjie Wang,Jiali Gao,Guocai Zhong

Molecular & cellular proteomics : MCP 22:100579 PubMed37211047

2023

A New Cellular Interactome of SARS-CoV-2 Nucleocapsid Protein and Its Biological Implications.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan-Qin Min,Mengzhuo Huang,Kuan Feng,Yajie Jia,Xiulian Sun,Yun-Jia Ning

Nature plants 9:96-111 PubMed36624255

2023

Cell-type-specific PtrWOX4a and PtrVCS2 form a regulatory nexus with a histone modification system for stem cambium development in Populus trichocarpa.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufang Dai,Rui Zhai,Jiaojiao Lin,Zhifeng Wang,Dekai Meng,Meng Li,Yuli Mao,Boyuan Gao,Hongyan Ma,Baofeng Zhang,Yi Sun,Shuang Li,Chenguang Zhou,Ying-Chung Jimmy Lin,Jack P Wang,Vincent L Chiang,Wei Li

The EMBO journal 41:e110777 PubMed35993436

2022

Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Volkmar,Christian M Gawden-Bone,James C Williamson,Jonathon Nixon-Abell,James A West,Peter H St George-Hyslop,Arthur Kaser,Paul J Lehner

Parasites & vectors 13:415 PubMed32787908

2020

Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice.

Applications

Unspecified application

Species

Unspecified reactive species

Samira Salari,Iraj Sharifi,Ali Reza Keyhani,Pooya Ghasemi Nejad Almani

The Plant cell 32:2823-2841 PubMed32699171

2020

A SnRK1-RFWD3-Opaque2 Signaling Axis Regulates Diurnal Nitrogen Accumulation in Maize Seeds.

Applications

Unspecified application

Species

Unspecified reactive species

Chaobin Li,Weiwei Qi,Zheng Liang,Xi Yang,Zeyang Ma,Rentao Song

Autophagy 15:1234-1257 PubMed30776962

2019

Histone acetyltransferase MoHat1 acetylates autophagy-related proteins MoAtg3 and MoAtg9 to orchestrate functional appressorium formation and pathogenicity in .

Applications

Unspecified application

Species

Unspecified reactive species

Ziyi Yin,Chen Chen,Jie Yang,Wanzhen Feng,Xinyu Liu,Rongfang Zuo,Jingzhen Wang,Lina Yang,Kaili Zhong,Chuyun Gao,Haifeng Zhang,Xiaobo Zheng,Ping Wang,Zhengguang Zhang

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