AB19321

Anti-S tag antibody

Lab Essentials
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(11 Publications)

Goat Polyclonal S tag antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 11 publications.

View Alternative Names

KETAAAKFERQHMDS tag antibody, S peptide, S peptide epitope tag

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Supplier Data

Western blot - Anti-S tag antibody (AB19321)

All lanes:

Western blot - Anti-S tag antibody (ab19321) at 0.04 µg/mL

Lane 1:

E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg

Lane 2:

E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg

Lane 3:

E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

Predicted band size: 18 kDa

false

Exposure time: 30s

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

ICC/IF, ICC, ELISA, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "Primary: 1/1000 - 1/30000.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.

Biological function summary

S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.

Pathways

S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.

S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

See full target information S tag

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 27:1666-1674.e4 PubMed31067454

2019

The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.

Applications

Unspecified application

Species

Unspecified reactive species

David L Lin,Takamasa Inoue,Yu-Jie Chen,Aaron Chang,Billy Tsai,Andrew W Tai

Molecular cell 74:729-741.e7 PubMed30982745

2019

Dual Role of Ribosome-Binding Domain of NAC as a Potent Suppressor of Protein Aggregation and Aging-Related Proteinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Koning Shen,Martin Gamerdinger,Rebecca Chan,Karina Gense,Esther M Martin,Nadine Sachs,Patrick D Knight,Renate Schlömer,Antonio N Calabrese,Katie L Stewart,Lukas Leiendecker,Ankit Baghel,Sheena E Radford,Judith Frydman,Elke Deuerling

PloS one 13:e0191052 PubMed29485998

2018

Identification and characterization of highly versatile peptide-vectors that bind non-competitively to the low-density lipoprotein receptor for in vivo targeting and delivery of small molecules and protein cargos.

Applications

ELISA, ICC/IF

Species

Unspecified reactive species, Unspecified reactive species

Marion David,Pascaline Lécorché,Maxime Masse,Aude Faucon,Karima Abouzid,Nicolas Gaudin,Karine Varini,Fanny Gassiot,Géraldine Ferracci,Guillaume Jacquot,Patrick Vlieghe,Michel Khrestchatisky

PLoS genetics 11:e1005297 PubMed26132230

2015

Reversible Oxidation of a Conserved Methionine in the Nuclear Export Sequence Determines Subcellular Distribution and Activity of the Fungal Nitrate Regulator NirA.

Applications

Species

Unspecified reactive species

Andreas Gallmetzer,Lucia Silvestrini,Thorsten Schinko,Bernd Gesslbauer,Peter Hortschansky,Christoph Dattenböck,María Isabel Muro-Pastor,Andreas Kungl,Axel A Brakhage,Claudio Scazzocchio,Joseph Strauss

Genetics 198:1513-33 PubMed25261697

2014

Translational control of the oogenic program by components of OMA ribonucleoprotein particles in Caenorhabditis elegans.

Applications

Species

Unspecified reactive species

Caroline A Spike,Donna Coetzee,Yuichi Nishi,Tugba Guven-Ozkan,Marieke Oldenbroek,Ikuko Yamamoto,Rueyling Lin,David Greenstein

The Journal of investigative dermatology 134:1589-1598 PubMed24499735

2014

Palmoplantar keratoderma along with neuromuscular and metabolic phenotypes in Slurp1-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Oludotun Adeyo,Bernard B Allan,Richard H Barnes,Chris N Goulbourne,Angelica Tatar,Yiping Tu,Lorraine C Young,Michael M Weinstein,Peter Tontonoz,Loren G Fong,Anne P Beigneux,Stephen G Young

Methods in molecular biology (Clifton, N.J.) 944:221-36 PubMed23065620

2012

Chromatin immunoprecipitation analysis in filamentous fungi.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Boedi,Yazmid Reyes-Dominguez,Joseph Strauss

Proceedings of the National Academy of Sciences of the United States of America 108:14282-7 PubMed21825172

2011

Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation.

Applications

Species

Unspecified reactive species

Hans-Wilhelm Nützmann,Yazmid Reyes-Dominguez,Kirstin Scherlach,Volker Schroeckh,Fabian Horn,Agnieszka Gacek,Julia Schümann,Christian Hertweck,Joseph Strauss,Axel A Brakhage

Proceedings of the National Academy of Sciences of the United States of America 108:7980-4 PubMed21518912

2011

Mutations in lipoprotein lipase that block binding to the endothelial cell transporter GPIHBP1.

Applications

ICC/IF

Species

Unspecified reactive species

Constance V Voss,Brandon S J Davies,Shelly Tat,Peter Gin,Loren G Fong,Christopher Pelletier,Charlene D Mottler,André Bensadoun,Anne P Beigneux,Stephen G Young

The Journal of biological chemistry 286:19735-43 PubMed21478160

2011

Assessing the role of the glycosylphosphatidylinositol-anchored high density lipoprotein-binding protein 1 (GPIHBP1) three-finger domain in binding lipoprotein lipase.

Applications

Species

Unspecified reactive species

Anne P Beigneux,Brandon S J Davies,Shelly Tat,Jenny Chen,Peter Gin,Constance V Voss,Michael M Weinstein,André Bensadoun,Clive R Pullinger,Loren G Fong,Stephen G Young

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