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Goat Polyclonal S tag antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 11 publications.

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Images

Western blot - Anti-S tag antibody (AB19321), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Goat
Storage buffer

pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Polyclonal

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCELISAWBICC/IF
Tag
Expected
Expected
Tested
Expected

Expected
Expected

Species
Tag
Dilution info
Use at an assay dependent concentration.
Notes

-

Expected
Expected

Species
Tag
Dilution info
-
Notes

Primary: 1/1000 - 1/30000.

Tested
Tested

Species
Tag
Dilution info
-
Notes

-

Expected
Expected

Species
Tag
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

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Alternative names

Recommended products

Goat Polyclonal S tag antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Purification technique
Affinity purification Immunogen
Concentration
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Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.

Biological function summary

S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.

Pathways

S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.

Associated diseases and disorders

S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - Anti-S tag antibody (ab19321), expandable thumbnail

    Western blot - Anti-S tag antibody (ab19321)

    All lanes: Western blot - Anti-S tag antibody (ab19321) at 0.04 µg/mL

    Lane 1: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg

    Lane 2: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg

    Lane 3: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg

    Predicted band size: 18 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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