Goat Polyclonal S tag antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 11 publications.
pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS
ICC | ELISA | WB | ICC/IF | |
---|---|---|---|---|
Tag | Expected | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info - | Notes Primary: 1/1000 - 1/30000. |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Tag | Dilution info Use at an assay dependent concentration. | Notes - |
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KETAAAKFERQHMDS tag antibody, S peptide, S peptide epitope tag
Goat Polyclonal S tag antibody. Suitable for ICC, ELISA, WB, ICC/IF and reacts with Tag samples. Cited in 11 publications.
pH: 6.8 - 7.4
Preservative: 0.1% Sodium azide
Constituents: PBS
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The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.
S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.
S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.
S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-S tag antibody (ab19321) at 0.04 µg/mL
Lane 1: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.2 µg
Lane 2: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.1 µg
Lane 3: E. coli whole cell lysate expressing a multi-tag fusion protein at 0.05 µg
Predicted band size: 18 kDa
Exposure time: 30s
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