AB180958

Anti-S tag antibody [EPR12996]

RabMAb
Recombinant
Lab Essentials
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal S tag antibody. Suitable for WB and reacts with Tag samples. Cited in 4 publications.

View Alternative Names

KETAAAKFERQHMDS tag antibody, S peptide, S peptide epitope tag

1 Images

Supplier Data

Western blot - Anti-S tag antibody [EPR12996] (AB180958)

All lanes:

Western blot - Anti-S tag antibody [EPR12996] (ab180958) at 1/5000 dilution

Lane 1:

Multi-tag fusion protein at 0.01 µg

Lane 2:

Multi-tag fusion protein at 0.005 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 18 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12996

Isotype

IgG

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S tag is a peptide sequence derived from ribonuclease A commonly used in molecular biology for protein purification and detection. This tag has a molecular mass of approximately 2 kDa. Researchers often utilize S tags for their small size which minimizes interference with protein function. The S tag is typically expressed as a fusion with the protein of interest which enables it to be used as a versatile tool in a variety of expression systems.

Biological function summary

S tags do not independently participate in cellular functions but instead facilitate experimental procedures. When fused to a protein the S tag allows for simple detection and purification using anti-S tag antibodies. This functionality makes it possible to isolate and study the protein of interest without affecting its native activity significantly. While S tags do not form part of any specific complex they interact with complexes like alkaline phosphatase in experimental applications to enhance detection signals.

Pathways

S tags themselves do not have inherent roles in cellular pathways. However they are used as a tool in studies involving signal transduction or metabolic pathways where their presence aids in tracking or manipulating pathway components. Due to their utility S tags can be connected indirectly to proteins such as ALP (alkaline phosphatase) by enabling pathway mapping in experiments.

S tags do not directly relate to specific conditions but are instrumental in research. For instance they allow scientists to study proteins connected to cancer or metabolic disorders more effectively. In research related to metabolic disorders S-tagged proteins can be linked to alkaline phosphatase helping in investigations to understand the role of alkaline phosphatase in calcium metabolism issues.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information S tag

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3789 PubMed38710693

2024

A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3.

Applications

Unspecified application

Species

Unspecified reactive species

Weize Wang,Ling Liang,Zonglin Dai,Peng Zuo,Shang Yu,Yishuo Lu,Dian Ding,Hongyi Chen,Hui Shan,Yan Jin,Youdong Mao,Yuxin Yin

Virus research 336:199194 PubMed37579847

2023

Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody.

Applications

Unspecified application

Species

Unspecified reactive species

Liwei Li,Sina Qiao,Shumao Wang,Jiachen Liu,Kuan Zhao,Yanjun Zhou,Guoxin Li,Yifeng Jiang,Changlong Liu,Guangzhi Tong,Wu Tong,Fei Gao

Nature communications 14:876 PubMed36797319

2023

Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Edwards,Isaac Njaci,Abhimanyu Sarkar,Zhouqian Jiang,Gemy George Kaithakottil,Christopher Moore,Jitender Cheema,Clare E M Stevenson,Martin Rejzek,Petr Novák,Marielle Vigouroux,Martin Vickers,Roland H M Wouters,Pirita Paajanen,Burkhard Steuernagel,Jonathan D Moore,Janet Higgins,David Swarbreck,Stefan Martens,Colin Y Kim,Jing-Ke Weng,Sagadevan Mundree,Benjamin Kilian,Shiv Kumar,Matt Loose,Levi Yant,Jiří Macas,Trevor L Wang,Cathie Martin,Peter M F Emmrich

Channels (Austin, Tex.) 14:268-286 PubMed32815768

2020

Calmodulin binds to the N-terminal domain of the cardiac sodium channel Na1.5.

Applications

Unspecified application

Species

Unspecified reactive species

Zizun Wang,Sarah H Vermij,Valentin Sottas,Anna Shestak,Daniela Ross-Kaschitza,Elena V Zaklyazminskaya,Andy Hudmon,Geoffrey S Pitt,Jean-Sébastien Rougier,Hugues Abriel

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