AB134149

Anti-S100 alpha 6/PRA antibody [EPNCIR121]

RabMAb
Recombinant
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(7 Publications)

Rabbit Recombinant Monoclonal S100 alpha 6/PRA antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 7 publications.

View Alternative Names

CACY, S100A6, Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

Immunocytochemistry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling S100 alpha 6/PRA with purified ab134149 at 1/50 dilution (2.5 μg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/mL). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Flow Cytometry (Intracellular) - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labelling S100 alpha 6/PRA with purified ab134149 at 1/100 dilution (1 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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Western blot - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

All lanes:

Western blot - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (ab134149) at 1/5000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate

Lane 2:

C6 (Rat glial tumor glial cell) whole cell lysate

Lane 3:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPNCIR121

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/1000", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha 6 also known as PRA is a member of the S100 protein family characterized by its small molecular weight of approximately 10 kDa. This protein expresses in various tissues including the heart skeletal muscle kidney and brain. The S100 family proteins commonly contain two EF-hand calcium-binding motifs which play key roles in intracellular and extracellular regulatory activities. S100 alpha 6 participates in calcium-dependent signal transduction processes that affect a range of cellular functions.

Biological function summary

S100 alpha 6 plays a significant role in intracellular calcium signaling and cytoskeletal dynamics. This protein often interacts as a part of protein complexes with various other proteins including annexin and tropomyosin contributing to vital cellular functions like contraction motility and cell cycle regulation. Its expression levels and spatial distribution influence cellular proliferation and differentiation making it an important component in several cell types.

Pathways

The protein S100 alpha 6 participates in essential pathways such as the MAPK and calcium signaling pathways. In the MAPK pathway S100 alpha 6 interacts with proteins like ERK1/2 influencing cell growth and apoptosis signaling cascades. In calcium signaling it helps regulate the release and uptake of calcium ions vital for maintaining cellular homeostasis and function. Through these pathways S100 alpha 6 is interconnected with other proteins like calmodulin and calcyclin which share similar physiological roles.

Increased or decreased expression of S100 alpha 6 relates to conditions such as cancer and cardiomyopathy. In cancer S100 alpha 6 often associates with dysregulated cell growth and can influence tumor progression through its interactions with proteins such as p53 and MDM2. In cardiomyopathy it affects calcium-handling properties in heart muscle cells linking it to cardiac dysfunction. These associations highlight its impact on disease development and progression making it a target for research in therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.

See full target information S100A6

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of molecular medicine (Berlin, Germany) 101:1015-1028 PubMed37462767

2023

Novel insights into the nervous system affected by prolonged hyperglycemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kamila Zglejc-Waszak,Konark Mukherjee,Agnieszka Korytko,Bogdan Lewczuk,Andrzej Pomianowski,Joanna Wojtkiewicz,Marta Banach,Michał Załęcki,Natalia Nowicka,Julia Jarosławska,Bernard Kordas,Krzysztof Wąsowicz,Judyta K Juranek

Molecular oncology 15:2011-2025 PubMed33932101

2021

Tumour-derived substrate-adherent cells promote neuroblastoma survival through secreted trophic factors.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Yubing Wang,Lisha Li,Penelope M-Y Or,Chi Wai Wong,Tian Liu,Wayne L H Ho,Andrew M Chan

Nature communications 11:1617 PubMed32238817

2020

The translational landscape of ground state pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Yaser Atlasi,Seyed Mehdi Jafarnejad,Christos G Gkogkas,Michiel Vermeulen,Nahum Sonenberg,Hendrik G Stunnenberg

Cells 8: PubMed31137713

2019

Single Cell RNA Sequencing Identifies Subsets of Hepatic Stellate Cells and Myofibroblasts in Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Krenkel,Jana Hundertmark,Thomas P Ritz,Ralf Weiskirchen,Frank Tacke

Nature immunology 18:184-195 PubMed27992400

2016

The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Semir Beyaz,Ji Hyung Kim,Luca Pinello,Michael E Xifaras,Yu Hu,Jialiang Huang,Marc A Kerenyi,Partha P Das,R Anthony Barnitz,Aurelie Herault,Rizkullah Dogum,W Nicholas Haining,Ömer H Yilmaz,Emmanuelle Passegue,Guo-Cheng Yuan,Stuart H Orkin,Florian Winau

The Journal of biological chemistry 288:7158-68 PubMed23344957

2013

Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein).

Applications

Species

Unspecified reactive species

Seiko Shimamoto,Yasuo Kubota,Fuminori Yamaguchi,Hiroshi Tokumitsu,Ryoji Kobayashi

Journal of biochemistry 153:209-20 PubMed23225596

2012

iTRAQ-based quantitative proteomic analysis of submandibular glands from rats with STZ-induced hyperglycemia.

Applications

Species

Rat

Renato M P Alves,Rui Vitorino,Ana I Padrão,Daniel Moreira-Gonçalves,José A Duarte,Rita M P Ferreira,Francisco Amado

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 alpha 6/PRA antibody [EPNCIR121]

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

Flow Cytometry (Intracellular) - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

Western blot - Anti-S100 alpha 6/PRA antibody [EPNCIR121] (AB134149)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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