Anti-S100 alpha 6/PRA antibody [EPR13084-69]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

Immunocytochemistry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling S100 alpha 6/PRA with purified ab181975 at 1 : 250 dilution (0.66 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling S100 alpha 6/PRA with Purified ab181975 at 1 : 4000 (0.04 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue sections labeling S100 alpha 6/PRA with Purified ab181975 at 1 : 4000 (0.04 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling S100 alpha 6/PRA with purified ab181975 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling S100 alpha 6/PRA with Purified ab181975 at 1 : 4000 (0.04 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IP

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Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

Purified ab181975 at 1/20 dilution (0.8 μg) immunoprecipitating S100 alpha 6/PRA in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg.
Lane 2 (+) : ab181975 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181975 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 10 kDa

All lanes:

Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975)

Predicted band size: 10 kDa

false

• WB

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Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

All lanes:

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

Human lung lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

• WB

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Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (AB181975)

All lanes:

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (ab181975) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 20 µg

Lane 2:

Mouse kidney lysate at 20 µg

Lane 3:

Mouse spleen lysate at 20 µg

Lane 4:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Lane 5:

Rat kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false