Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Rabbit Recombinant Monoclonal S100 alpha 6/PRA antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (AB250543), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Expected
Rat	Expected	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Target data

See full target information S100A6

Function

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.

Alternative names

CACY, S100A6, Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA

Recommended products

Rabbit Recombinant Monoclonal S100 alpha 6/PRA antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR13084-69
Purification technique: Affinity purification Protein A
Specificity: The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab250543 is the carrier-free version of Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

S100 alpha 6 also known as PRA is a member of the S100 protein family characterized by its small molecular weight of approximately 10 kDa. This protein expresses in various tissues including the heart skeletal muscle kidney and brain. The S100 family proteins commonly contain two EF-hand calcium-binding motifs which play key roles in intracellular and extracellular regulatory activities. S100 alpha 6 participates in calcium-dependent signal transduction processes that affect a range of cellular functions.

Biological function summary

S100 alpha 6 plays a significant role in intracellular calcium signaling and cytoskeletal dynamics. This protein often interacts as a part of protein complexes with various other proteins including annexin and tropomyosin contributing to vital cellular functions like contraction motility and cell cycle regulation. Its expression levels and spatial distribution influence cellular proliferation and differentiation making it an important component in several cell types.

Pathways

The protein S100 alpha 6 participates in essential pathways such as the MAPK and calcium signaling pathways. In the MAPK pathway S100 alpha 6 interacts with proteins like ERK1/2 influencing cell growth and apoptosis signaling cascades. In calcium signaling it helps regulate the release and uptake of calcium ions vital for maintaining cellular homeostasis and function. Through these pathways S100 alpha 6 is interconnected with other proteins like calmodulin and calcyclin which share similar physiological roles.

Associated diseases and disorders

Increased or decreased expression of S100 alpha 6 relates to conditions such as cancer and cardiomyopathy. In cancer S100 alpha 6 often associates with dysregulated cell growth and can influence tumor progression through its interactions with proteins such as p53 and MDM2. In cardiomyopathy it affects calcium-handling properties in heart muscle cells linking it to cardiac dysfunction. These associations highlight its impact on disease development and progression making it a target for research in therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling S100 alpha 6/PRA with purified Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 at 1:4000 (0.04 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
Purified Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 at 1/20 dilution (0.8 μg) immunoprecipitating S100 alpha 6/PRA in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg.
Lane 2 (+): Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 10 kDa
All lanes: Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975)
Predicted band size: 10 kDa
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling S100 alpha 6/PRA with purified Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 at 1:250 dilution (0.66 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (2.5 µg/ml) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red). Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as a nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975) at 1/1000 dilution
Lane 1: Mouse heart lysate at 20 µg
Lane 2: Mouse kidney lysate at 20 µg
Lane 3: Mouse spleen lysate at 20 µg
Lane 4: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 5: Rat kidney lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
Flow Cytometry (Intracellular) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling S100 alpha 6/PRA with purified Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue sections labeling S100 alpha 6/PRA with purified Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 at 1:4000 (0.04 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] (Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975) at 1/1000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 2: Human lung lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 10 kDa
Observed band size: 10 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)
This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling S100 alpha 6/PRA with purified Anti-S100 alpha 6/PRA antibody [EPR13084-69] ab181975 at 1:4000 (0.04 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

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Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free

Images

Publications

Key facts

Immunogen

Reactivity data

IP

TestedTested

ExpectedExpected

WB

TestedTested

ICC/IF

TestedTested

ExpectedExpected

Flow Cyt (Intra)

TestedTested

ExpectedExpected

IHC-P

TestedTested

ExpectedExpected

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

8 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Immunoprecipitation - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Flow Cytometry (Intracellular) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084-69] - BSA and Azide free (ab250543)

Downloads

Product protocols

Tested
Tested

Expected
Expected

Tested
Tested

Tested
Tested

Expected
Expected

Tested
Tested

Expected
Expected

Tested
Tested

Expected
Expected