AB250542

Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal S100 alpha 6/PRA antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

View Alternative Names

CACY, S100A6, Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

This data was developed using ab181974, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling S100 alpha 6/PRA with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green). Dapi staining (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

This data was developed using ab181974, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6/PRA with ab181974 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

This data was developed using ab181974, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of acetone-fixed HeLa cells labeling S100 alpha 6/PRA with ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/200 dilution (red). Dapi staining (blue).

Supplier Data

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

This data was developed using ab181974, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] (<a href='/en-us/products/primary-antibodies/s100-alpha-6-pra-antibody-epr13084-ab181974'>ab181974</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A549 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

Supplier Data

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

This data was developed using ab181974, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] (<a href='/en-us/products/primary-antibodies/s100-alpha-6-pra-antibody-epr13084-ab181974'>ab181974</a>) at 1/2000 dilution

All lanes:

Human fetal lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

Unconjugated

Anti-S100 alpha 6/PRA antibody [EPR13084]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13084

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250542 is the carrier-free version of ab181974.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha 6 also known as PRA is a member of the S100 protein family characterized by its small molecular weight of approximately 10 kDa. This protein expresses in various tissues including the heart skeletal muscle kidney and brain. The S100 family proteins commonly contain two EF-hand calcium-binding motifs which play key roles in intracellular and extracellular regulatory activities. S100 alpha 6 participates in calcium-dependent signal transduction processes that affect a range of cellular functions.

Biological function summary

S100 alpha 6 plays a significant role in intracellular calcium signaling and cytoskeletal dynamics. This protein often interacts as a part of protein complexes with various other proteins including annexin and tropomyosin contributing to vital cellular functions like contraction motility and cell cycle regulation. Its expression levels and spatial distribution influence cellular proliferation and differentiation making it an important component in several cell types.

Pathways

The protein S100 alpha 6 participates in essential pathways such as the MAPK and calcium signaling pathways. In the MAPK pathway S100 alpha 6 interacts with proteins like ERK1/2 influencing cell growth and apoptosis signaling cascades. In calcium signaling it helps regulate the release and uptake of calcium ions vital for maintaining cellular homeostasis and function. Through these pathways S100 alpha 6 is interconnected with other proteins like calmodulin and calcyclin which share similar physiological roles.

Increased or decreased expression of S100 alpha 6 relates to conditions such as cancer and cardiomyopathy. In cancer S100 alpha 6 often associates with dysregulated cell growth and can influence tumor progression through its interactions with proteins such as p53 and MDM2. In cardiomyopathy it affects calcium-handling properties in heart muscle cells linking it to cardiac dysfunction. These associations highlight its impact on disease development and progression making it a target for research in therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.

See full target information S100A6

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise