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Rabbit Recombinant Monoclonal S100 alpha 6/PRA antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542), expandable thumbnail
  • Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (AB250542), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

1 products for Alternative Version

    Target data

    Function

    May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes such as the reorganization of the actin cytoskeleton and in cell motility. Binds 2 calcium ions. Calcium binding is cooperative.

    Alternative names

    Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA, CACY, S100A6

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    Rabbit Recombinant Monoclonal S100 alpha 6/PRA antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human samples.

    Alternative names

    Protein S100-A6, Calcyclin, Growth factor-inducible protein 2A9, MLN 4, Prolactin receptor-associated protein, S100 calcium-binding protein A6, PRA, CACY, S100A6

    Key facts

    Isotype

    IgG

    Form

    Liquid

    Clonality

    Monoclonal

    Immunogen
    • The exact immunogen used to generate this antibody is proprietary information.
    Carrier free

    Yes

    Clone number

    EPR13084

    Purification technique

    Affinity purification Protein A

    Concentration
    Loading...

    Storage

    Shipped at conditions

    Blue Ice

    Appropriate long-term storage conditions

    +4°C

    Storage information

    Do Not Freeze

    Notes

    ab250542 is the carrier-free version of Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free batch production

    For more information, read more on recombinant antibodies.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Supplementary info

    Activity summary

    S100 alpha 6 also known as PRA is a member of the S100 protein family characterized by its small molecular weight of approximately 10 kDa. This protein expresses in various tissues including the heart skeletal muscle kidney and brain. The S100 family proteins commonly contain two EF-hand calcium-binding motifs which play key roles in intracellular and extracellular regulatory activities. S100 alpha 6 participates in calcium-dependent signal transduction processes that affect a range of cellular functions.

    Biological function summary

    S100 alpha 6 plays a significant role in intracellular calcium signaling and cytoskeletal dynamics. This protein often interacts as a part of protein complexes with various other proteins including annexin and tropomyosin contributing to vital cellular functions like contraction motility and cell cycle regulation. Its expression levels and spatial distribution influence cellular proliferation and differentiation making it an important component in several cell types.

    Pathways

    The protein S100 alpha 6 participates in essential pathways such as the MAPK and calcium signaling pathways. In the MAPK pathway S100 alpha 6 interacts with proteins like ERK1/2 influencing cell growth and apoptosis signaling cascades. In calcium signaling it helps regulate the release and uptake of calcium ions vital for maintaining cellular homeostasis and function. Through these pathways S100 alpha 6 is interconnected with other proteins like calmodulin and calcyclin which share similar physiological roles.

    Associated diseases and disorders

    Increased or decreased expression of S100 alpha 6 relates to conditions such as cancer and cardiomyopathy. In cancer S100 alpha 6 often associates with dysregulated cell growth and can influence tumor progression through its interactions with proteins such as p53 and MDM2. In cardiomyopathy it affects calcium-handling properties in heart muscle cells linking it to cardiac dysfunction. These associations highlight its impact on disease development and progression making it a target for research in therapeutic strategies.

    Product promise

    We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

    In the unlikely event of one of our products not working as expected, you are covered by our product promise.

    Full details and terms and conditions can be found here:
    Terms & Conditions.

    5 product images

    • Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542), expandable thumbnail

      Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542)

      This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974, the same antibody clone in a different buffer formulation.

      All lanes: Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] (Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974) at 1/2000 dilution

      Lane 1: HeLa cell lysate at 20 µg

      Lane 2: A549 cell lysate at 20 µg

      Secondary

      All lanes: Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

      Predicted band size: 10 kDa

      Observed band size: 10 kDa

    • Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542), expandable thumbnail

      Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542)

      This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974, the same antibody clone in a different buffer formulation.

      All lanes: Western blot - Anti-S100 alpha 6/PRA antibody [EPR13084] (Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974) at 1/2000 dilution

      All lanes: Human fetal lung tissue lysate at 20 µg

      Secondary

      All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

      Predicted band size: 10 kDa

      Observed band size: 10 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542), expandable thumbnail

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542)

      This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue labeling S100 alpha 6/PRA with Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974 at 1/50 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542)

      This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling S100 alpha 6/PRA with Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 488) at 1/200 dilution (green). Dapi staining (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542), expandable thumbnail

      Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha 6/PRA antibody [EPR13084] - BSA and Azide free (ab250542)

      This data was developed using Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling S100 alpha 6/PRA with Anti-S100 alpha 6/PRA antibody [EPR13084] ab181974 at 1/500 dilution, followed by Goat anti rabbit IgG (Dylight 555) at 1/200 dilution (red). Dapi staining (blue).

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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