AB52271

Anti-S100 alpha antibody [2C8B8]

Name: Anti-S100 alpha antibody [2C8B8] (ab52271) | Abcam
Brand: Abcam Limited
SKU: AB52271

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(1 Publication)

Mouse Monoclonal S10A1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human S100A1.

View Alternative Names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [2C8B8] (AB52271)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [2C8B8] (AB52271)

ab52271 staining Human lymphoid follicle tissue; paraffin embedded.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [2C8B8] (AB52271)

ab52271 staining human brain tissue; paraffin embedded.

Unknown

Western blot - Anti-S100 alpha antibody [2C8B8] (AB52271)

All lanes:

Western blot - Anti-S100 alpha antibody [2C8B8] (ab52271)

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Molecular weight markers

Lane 2:

Truncated S100A recombinant protein

Predicted band size: 11 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2C8B8

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human S100A1. The exact immunogen used to generate this antibody is proprietary information.

P23297

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha also known as S100A1 among other S100 family proteins plays a role in calcium ion binding which influences various cellular processes. It is a small protein with a molecular mass around 10-12 kDa. S100 alpha expresses abundantly in the brain heart and skeletal muscle indicating its functional importance in these tissues.

Biological function summary

The S100 calcium-binding proteins like S100 alpha modulate the function of target proteins through interactions dependent on the calcium bound state. S100 alpha does not commonly form larger complexes but instead interacts directly with other proteins to exert its effects. Importantly it regulates activities such as phosphorylation enzyme activity and cellular proliferation and differentiation.

Pathways

S100 alpha integrates into the intracellular pathways that mediate calcium signaling and respond to oxidative stress. Notably it is involved in the regulation of the RAGE (Receptor for Advanced Glycation End-products) pathway and related NF-kB pathway both of which lead to inflammatory responses. Through these pathways S100 alpha is related to several proteins like p53 and those involved in cytoskeletal dynamics.

S100 alpha demonstrates relevance in conditions linked to aberrant calcium signaling and inflammation such as cardiomyopathy and certain neurodegenerative disorders. In cardiomyopathy alterations of S100 alpha affect cardiac contractility and contribute to heart failure. In neurodegenerative disorders the protein's interaction with RAGE and subsequent pathways possibly accelerates neuronal damage. The protein links with other S100 family members such as S100B further influencing disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).

See full target information S100A1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 19:4820-36 PubMed20858595

2010

A centronuclear myopathy-dynamin 2 mutation impairs skeletal muscle structure and function in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Anne-Cécile Durieux,Alban Vignaud,Bernard Prudhon,Mai Thao Viou,Maud Beuvin,Stéphane Vassilopoulos,Bodvaël Fraysse,Arnaud Ferry,Jeanne Lainé,Norma B Romero,Pascale Guicheney,Marc Bitoun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 alpha antibody [2C8B8]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [2C8B8] (AB52271)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [2C8B8] (AB52271)

Western blot - Anti-S100 alpha antibody [2C8B8] (AB52271)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise