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AB183979

Anti-S100 alpha antibody [EPR19013]

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(9 Publications)

Rabbit Recombinant Monoclonal S10A1 antibody. Suitable for IP, WB, IHC-Fr, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 9 publications.

View Alternative Names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Part of kidney tubules were strongly staining. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on glial cells and negative on neuron cells of Human cerebrum was observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive on germinal center of Human tonsil. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-S100 alpha antibody [EPR19013] (AB183979)

C2C12 (mouse muscle) cells were fixed with 4% paraformaldehyde and incubated with the primary antibody (ab183979; red line) diluted 1/60 (1μg). The secondary antibody used was Goat anti-rabbit IgG (Alexa Fluor®488) at 1/500 dilution. The isotype control (black line) antibody wasRabbit monoclonal IgG (ab172730). An unlabelled control without incubation with primary antibody and secondary antibody was also perfomed (blue line).

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling S100 with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse kidney. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain tissue labeling S100 with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on glial cells of mouse cortex. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] (AB183979)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling S100 with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse spleen. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • IP

Supplier Data

Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] (AB183979)

S100 was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab183979 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183979 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : RAW 264.7 whole cell lysate, 10μg (Input).

Lane 2 : ab183979 IP in RAW 264.7 whole cell lysate.

Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183979 in RAW 264.7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] (ab183979)

Predicted band size: 11 kDa

false

Western blot - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • WB

Supplier Data

Western blot - Anti-S100 alpha antibody [EPR19013] (AB183979)

Blocking/Dilution buffer : 5% NFDM/TBST.

Note that high molecular weight bands were detected in mouse tissue lysates.

All lanes:

Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979) at 1/2000 dilution

Lane 1:

Human skeletal muscle lysate at 20 µg

Lane 2:

Mouse cerebral cortex lysate at 20 µg

Lane 3:

Mouse liver lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 3min

Western blot - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • WB

Supplier Data

Western blot - Anti-S100 alpha antibody [EPR19013] (AB183979)

Blocking/Dilution buffer : 5% NFDM/TBST.

Note that high molecular weight bands were detected in mouse tissue lysates.

All lanes:

Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979) at 1/1000 dilution

Lane 1:

Mouse muscle lysate at 10 µg

Lane 2:

Mouse heart lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 3min

Western blot - Anti-S100 alpha antibody [EPR19013] (AB183979)
  • WB

Supplier Data

Western blot - Anti-S100 alpha antibody [EPR19013] (AB183979)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-S100 alpha antibody [EPR19013] (ab183979) at 1/2000 dilution

All lanes:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 11 kDa

Observed band size: 11 kDa

false

Exposure time: 3min

  • Carrier free

    Anti-S100 alpha antibody [EPR19013] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19013

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), IHC-Fr, IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>Antigen retrieval: Heated citrate solution (10mM citrate pH 6.0 + 0.05% Tween-20). Weak positive staining was observed in mouse with minimal background.</p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/60", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha also known as S100A1 among other S100 family proteins plays a role in calcium ion binding which influences various cellular processes. It is a small protein with a molecular mass around 10-12 kDa. S100 alpha expresses abundantly in the brain heart and skeletal muscle indicating its functional importance in these tissues.
Biological function summary

The S100 calcium-binding proteins like S100 alpha modulate the function of target proteins through interactions dependent on the calcium bound state. S100 alpha does not commonly form larger complexes but instead interacts directly with other proteins to exert its effects. Importantly it regulates activities such as phosphorylation enzyme activity and cellular proliferation and differentiation.

Pathways

S100 alpha integrates into the intracellular pathways that mediate calcium signaling and respond to oxidative stress. Notably it is involved in the regulation of the RAGE (Receptor for Advanced Glycation End-products) pathway and related NF-kB pathway both of which lead to inflammatory responses. Through these pathways S100 alpha is related to several proteins like p53 and those involved in cytoskeletal dynamics.

S100 alpha demonstrates relevance in conditions linked to aberrant calcium signaling and inflammation such as cardiomyopathy and certain neurodegenerative disorders. In cardiomyopathy alterations of S100 alpha affect cardiac contractility and contribute to heart failure. In neurodegenerative disorders the protein's interaction with RAGE and subsequent pathways possibly accelerates neuronal damage. The protein links with other S100 family members such as S100B further influencing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).
See full target information S100A1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastrointestinal and liver diseases : JGLD 34:30-39 PubMed40153818

2025

Programmed Cell Death-ligand 1 as Biomarker of Poor Prognosis in Patients with Gastrointestinal Stromal Tumour.

Applications

Unspecified application

Species

Unspecified reactive species

Duolikun Yasheng,Abuduwaili Aierken,Yiliang Li,Aikebaier Aili,Kelimu Abudureyimu

Biomedical reports 22:27 PubMed39720298

2024

Typical presentation of neurofibromatosis type I in a patient with giant cutaneous neoplasm and café au lait spots: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Chenguang Zhang,Yilina Saibaidula,Yulou Luo,Jianghua Ou

Genesis (New York, N.Y. : 2000) 60:e23488 PubMed35765931

2022

Generation of an Ihh-mKate2-Dre knock-in mouse line.

Applications

Unspecified application

Species

Unspecified reactive species

Rongyu Li,Yunting Cai,Huisang Lin,Lei Dong,Mingchuan Tang,Yiming Lang,Yini Qi,Yanli Peng,Bin Zhou,Guan Yang,Yan Teng,Xiao Yang

Cancer science 112:2233-2244 PubMed33743547

2021

Ppp6c haploinsufficiency accelerates UV-induced BRAF(V600E)-initiated melanomagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kosuke Kanazawa,Kazuhiro Kishimoto,Miyuki Nomura,Koreyuki Kurosawa,Hiroyuki Kato,Yui Inoue,Koh Miura,Katsuya Fukui,Yoji Yamashita,Ikuro Sato,Hiroyuki Tsuji,Toshio Watanabe,Takuji Tanaka,Jun Yasuda,Nobuhiro Tanuma,Hiroshi Shima

International journal of medical sciences 18:2008-2016 PubMed33850471

2021

Increased nuclear translation of YAP might act as a potential therapeutic target for NF1-related plexiform neurofibroma.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Liang Liu,Yuan-He You,Zhuo-Wei Tian,Meng Xiao,Jia-Wei Zheng,Yan-An Wang,Zhong Du

Journal of vascular research :1-16 PubMed33535226

2021

Reciprocal Antagonism between MicroRNA-138 and SIRT1 and Its Implications for the Angiogenesis of Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shangyu Wang,Zengwu Shao,Xin Tang,Kaijie Wang,Jinping Zhao,Zhe Dong

Scientific reports 11:1314 PubMed33446752

2021

Combined transcriptomic and lipidomic analysis reveals aberrant lipid metabolism in central nervous system hemangioblastomas.

Applications

Unspecified application

Species

Unspecified reactive species

Qiguang Wang,Wenke Liu,Si Zhang,Zuoyu Liang,Linhong Jiang,Aiqin Xue,Xiaobo Cen,Qian Bu

Neural regeneration research 15:1903-1911 PubMed32246639

2020

Combination of olfactory ensheathing cells and human umbilical cord mesenchymal stem cell-derived exosomes promotes sciatic nerve regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Wen-Tao Wang,Chun-Rong Gong,Chao Li,Mei Shi

Nature communications 8:1466 PubMed29133867

2017

Evidence of renal angiomyolipoma neoplastic stem cells arising from renal epithelial cells.

Applications

IHC-P

Species

Unspecified reactive species

Ana Filipa Gonçalves,Mojca Adlesic,Simone Brandt,Tomas Hejhal,Sabine Harlander,Lukas Sommer,Olga Shakhova,Peter J Wild,Ian J Frew
View all publications
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