Anti-S100 alpha antibody [EPR19013] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (AB227580)

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling S100 alpha with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on glial cells and negative on neuron cells of Human cerebrum was observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (AB227580)

This IHC data was generated using the same anti-S100 alpha antibody clone, EPR19013, in a different buffer formulation (cat# ab183979).

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive on germinal center of Human tonsil. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (AB227580)

This IHC data was generated using the same anti-S100 alpha antibody clone, EPR19013, in a different buffer formulation (cat# ab183979).

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling S100 with ab183979 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Part of kidney tubules were strongly staining. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (AB227580)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling S100 alpha with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse kidney. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (AB227580)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse brain tissue labeling S100 alpha with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on glial cells of mouse cortex. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (AB227580)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling S100 alpha with ab183979 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed positive staining on mouse spleen. The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

• IP

Supplier Data

Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] - BSA and Azide free (AB227580)

S100 alpha was immunoprecipitated from RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate with ab183979 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab183979 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : RAW 264.7 whole cell lysate, 10μg (Input).

Lane 2 : ab183979 IP in RAW 264.7 whole cell lysate.

Lane 3 : Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab183979 in RAW 264.7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183979).

All lanes:

Immunoprecipitation - Anti-S100 alpha antibody [EPR19013] (<a href='/en-us/products/primary-antibodies/s100-alpha-antibody-epr19013-ab183979'>ab183979</a>)

Predicted band size: 11 kDa

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