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AB248534

Anti-S100 alpha antibody [EPR5250] - BSA and Azide free

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Rabbit Recombinant Monoclonal S10A1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples.

View Alternative Names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skeletal muscle tissue sections labeling S100 alpha with purified ab133519 at 1 : 100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of paraffin embedded Human endometrial carcinoma tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human glioma tissue sections labeling S100 alpha with purified ab133519 at 1 : 100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of paraffin embedded Human heart muscle tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Immunohistochemistry analysis of paraffin embedded Mouse heart tissue labelling S100 alpha with unpurified ab133519 at 1/100 dilution.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1 : 100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebral cortex tissue sections labeling S100 alpha with purified ab133519 at 1 : 100 dilution (4.33 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1 : 500 dilution. PBS instead of the primary antibody was used as the negative control.

Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • WB

Lab

Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-S100 alpha antibody [EPR5250] (<a href='/en-us/products/primary-antibodies/s100-alpha-antibody-epr5250-ab133519'>ab133519</a>) at 1/1000 dilution

Lane 1:

Rat heart lysates at 20 µg

Lane 2:

Human fetal heart lysates at 15 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 11 kDa

false

Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • WB

Lab

Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-S100 alpha antibody [EPR5250] (<a href='/en-us/products/primary-antibodies/s100-alpha-antibody-epr5250-ab133519'>ab133519</a>) at 1/1000 dilution

All lanes:

Mouse heart lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 10 kDa

false

Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)
  • WB

Unknown

Western blot - Anti-S100 alpha antibody [EPR5250] - BSA and Azide free (AB248534)

This data was developed using ab133519, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-S100 alpha antibody [EPR5250] (<a href='/en-us/products/primary-antibodies/s100-alpha-antibody-epr5250-ab133519'>ab133519</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Mouse brain tissue lysate at 10 µg

Lane 3:

C2C12 cell lysate at 10 µg

Secondary

All lanes:

HRP-labelled Goat anti-Rabbit at 1/2000 dilution

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5250

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642)

  • 4
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Primary Antibodies

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Secondary Antibodies

AB150077

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab248534 is the carrier-free version of ab133519.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 alpha also known as S100A1 among other S100 family proteins plays a role in calcium ion binding which influences various cellular processes. It is a small protein with a molecular mass around 10-12 kDa. S100 alpha expresses abundantly in the brain heart and skeletal muscle indicating its functional importance in these tissues.
Biological function summary

The S100 calcium-binding proteins like S100 alpha modulate the function of target proteins through interactions dependent on the calcium bound state. S100 alpha does not commonly form larger complexes but instead interacts directly with other proteins to exert its effects. Importantly it regulates activities such as phosphorylation enzyme activity and cellular proliferation and differentiation.

Pathways

S100 alpha integrates into the intracellular pathways that mediate calcium signaling and respond to oxidative stress. Notably it is involved in the regulation of the RAGE (Receptor for Advanced Glycation End-products) pathway and related NF-kB pathway both of which lead to inflammatory responses. Through these pathways S100 alpha is related to several proteins like p53 and those involved in cytoskeletal dynamics.

S100 alpha demonstrates relevance in conditions linked to aberrant calcium signaling and inflammation such as cardiomyopathy and certain neurodegenerative disorders. In cardiomyopathy alterations of S100 alpha affect cardiac contractility and contribute to heart failure. In neurodegenerative disorders the protein's interaction with RAGE and subsequent pathways possibly accelerates neuronal damage. The protein links with other S100 family members such as S100B further influencing disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).
See full target information S100A1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com