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Rabbit Recombinant Monoclonal S10A1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Mouse
Tested
Not recommended
Expected
Rat
Tested
Not recommended
Expected

Tested
Tested

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Not recommended
Not recommended

Species

Mouse, Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

-

Expected
Expected

Species

Mouse

Dilution info

1/100 - 1/250

Notes

-

Species

Rat

Dilution info

1/100 - 1/250

Notes

-

Target data

Function

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).

Alternative names

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Rabbit Recombinant Monoclonal S10A1 antibody. Suitable for WB, ICC/IF and reacts with Rat, Mouse, Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR5251

Purification technique

Affinity purification Protein A

Dissociation constant

5 x 10-11 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

Biological function summary

The S100 calcium-binding proteins like S100 alpha modulate the function of target proteins through interactions dependent on the calcium bound state. S100 alpha does not commonly form larger complexes but instead interacts directly with other proteins to exert its effects. Importantly it regulates activities such as phosphorylation enzyme activity and cellular proliferation and differentiation.

Activity summary

S100 alpha also known as S100A1 among other S100 family proteins plays a role in calcium ion binding which influences various cellular processes. It is a small protein with a molecular mass around 10-12 kDa. S100 alpha expresses abundantly in the brain heart and skeletal muscle indicating its functional importance in these tissues.

Pathways

S100 alpha integrates into the intracellular pathways that mediate calcium signaling and respond to oxidative stress. Notably it is involved in the regulation of the RAGE (Receptor for Advanced Glycation End-products) pathway and related NF-kB pathway both of which lead to inflammatory responses. Through these pathways S100 alpha is related to several proteins like p53 and those involved in cytoskeletal dynamics.

Associated diseases and disorders

S100 alpha demonstrates relevance in conditions linked to aberrant calcium signaling and inflammation such as cardiomyopathy and certain neurodegenerative disorders. In cardiomyopathy alterations of S100 alpha affect cardiac contractility and contribute to heart failure. In neurodegenerative disorders the protein's interaction with RAGE and subsequent pathways possibly accelerates neuronal damage. The protein links with other S100 family members such as S100B further influencing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384), expandable thumbnail

    Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Western blot - Anti-S100 alpha antibody [EPR5251] (AB109384) at 1/1000 dilution

    All lanes: C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 11 kDa

    Observed band size: 11 kDa

    Exposure time: 60s

    Blocking and diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using ab109384, the same antibody clone in a different buffer formulation.

  • Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384), expandable thumbnail

    Western blot - Anti-S100 alpha antibody [EPR5251] (ab109384)

    All lanes: Western blot - Anti-S100 alpha antibody [EPR5251] (AB109384) at 1/1000 dilution

    Lane 1: Rat kidney tissue lysate at 10 µg

    Lane 2: C2C12 cell lysate at 10 µg

    Lane 3: Mouse kidney tissue lysate at 10 µg

    Lane 4: Human brain lysate at 10 µg

    Lane 5: HT-1080 lysate at 10 µg

    Lane 6: Mouse heart tissue lysate at 10 µg

    Predicted band size: 11 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [EPR5251] (ab109384)

    ab109384, at a 1/100 dilution staining S100 alpha in HeLa cells

  • OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (ab109384), expandable thumbnail

    OI-RD Scanning - Anti-S100 alpha antibody [EPR5251] (ab109384)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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