Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)

Rabbit Recombinant Multiclonal S10A1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (AB317635), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC/IF	WB	IP	IHC-P	Flow Cyt (Intra)
Human	Tested	Tested	Not recommended	Tested	Not recommended
Mouse	Tested	Tested	Not recommended	Not recommended	Not recommended
Rat	Expected	Not recommended	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info -	Notes -

Target data

See full target information Protein S100-A1

Function

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).

Alternative names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

Recommended products

Rabbit Recombinant Multiclonal S10A1 antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: RM1154
Purification technique: Affinity purification Protein A
Specificity: Unsuitable for mouse IHC-P and rat WB.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab317635 is the carrier-free version of Anti-S100 alpha antibody [RM1154] ab317634.

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

S100 alpha also known as S100A1 among other S100 family proteins plays a role in calcium ion binding which influences various cellular processes. It is a small protein with a molecular mass around 10-12 kDa. S100 alpha expresses abundantly in the brain heart and skeletal muscle indicating its functional importance in these tissues.

Biological function summary

The S100 calcium-binding proteins like S100 alpha modulate the function of target proteins through interactions dependent on the calcium bound state. S100 alpha does not commonly form larger complexes but instead interacts directly with other proteins to exert its effects. Importantly it regulates activities such as phosphorylation enzyme activity and cellular proliferation and differentiation.

Pathways

S100 alpha integrates into the intracellular pathways that mediate calcium signaling and respond to oxidative stress. Notably it is involved in the regulation of the RAGE (Receptor for Advanced Glycation End-products) pathway and related NF-kB pathway both of which lead to inflammatory responses. Through these pathways S100 alpha is related to several proteins like p53 and those involved in cytoskeletal dynamics.

Associated diseases and disorders

S100 alpha demonstrates relevance in conditions linked to aberrant calcium signaling and inflammation such as cardiomyopathy and certain neurodegenerative disorders. In cardiomyopathy alterations of S100 alpha affect cardiac contractility and contribute to heart failure. In neurodegenerative disorders the protein's interaction with RAGE and subsequent pathways possibly accelerates neuronal damage. The protein links with other S100 family members such as S100B further influencing disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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14 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: no staining on rat liver. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression tissue: almost no staining on human liver. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat kidney. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat spleen. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on rat cerebrum. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human kidney. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/4000 (0.124 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human cerebrum. The section was incubated with Anti-S100 alpha antibody [RM1154] ab317634 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in RAW 264.7 cell line (shown in green).

Negative control: NIH/3T3.

The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Immunocytochemistry/ Immunofluorescence - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SK-MEL-2 (human skin malignant melanoma cell) cells labelling S100 alpha with Anti-S100 alpha antibody [RM1154] ab317634 at 1/50 (9.88 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green).
Confocal image showing cytoplasmic and nuclear staining in SK-MEL-2 cell line (shown in green).

Negative control: Jurkat.

The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.
Western blot - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Negative control: NIH/3T3.
Lane 3: The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-S100 alpha antibody [RM1154] (Anti-S100 alpha antibody [RM1154] ab317634) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: NIH/3T3 (mouse embryonic fibroblast) ) whole cell lysate at 20 µg with NFDM/TBST
Lane 3: RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) ) whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 11 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Low expression: liver.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lane 1/4: 180 seconds Lane 2-3: 59 seconds
All lanes: Western blot - Anti-S100 alpha antibody [RM1154] (Anti-S100 alpha antibody [RM1154] ab317634) at 1/1000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg with NFDM/TBST
Lane 2: Mouse kidney tissue lysate at 20 µg with NFDM/TBST
Lane 3: Mouse heart tissue lysate at 20 µg with NFDM/TBST
Lane 4: Mouse liver tissue lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 11 kDa, 36 kDa
Western blot - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Low expression: liver and spleen.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-S100 alpha antibody [RM1154] (Anti-S100 alpha antibody [RM1154] ab317634) at 1/1000 dilution
Lane 1: Human cerebellum tissue lysate at 20 µg with NFDM/TBST
Lane 2: Human liver tissue lysate at 20 µg with NFDM/TBST
Lane 3: Human kidney tissue lysate at 20 µg with NFDM/TBST
Lane 4: Human spleen tissue lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 11 kDa, 36 kDa
Exposure time: 180s
Western blot - Anti-S100 alpha antibody [RM1154] - BSA and Azide free (ab317635)
This data was developed using Anti-S100 alpha antibody [RM1154] ab317634, the same antibody clone in a different buffer formulation.
Negative control: Jurkat.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-S100 alpha antibody [RM1154] (Anti-S100 alpha antibody [RM1154] ab317634) at 1/1000 dilution
Lane 1: SK-MEL-2 (human skin malignant melanoma cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 2: SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 20 µg with NFDM/TBST
Lane 3: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg with NFDM/TBST
Lane 4: C2C12 (mouse myoblast) whole cell lysate at 20 µg with NFDM/TBST
Lane 5: C8-D1A (mouse cerebellum astrocyte) whole cell lysate at 20 µg with NFDM/TBST
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 11 kDa, 36 kDa
Exposure time: 180s