AB4066

Anti-S100 antibody [4C4.9]

Name: Anti-S100 antibody [4C4.9] (ab4066) | Abcam
Brand: Abcam Limited
SKU: AB4066
Price: 400 USD
Availability: InStock
Rating: 4 (12 reviews)

(12 Reviews)

(102 Publications)

Anti-S100 antibody [4C4.9] (ab4066) is a mouse monoclonal antibody detecting S100 in IHC-P. Suitable for Human.

- Over 80 publications
- Trusted since 2003

View Alternative Names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066)

Immunohistochemistry of Formalin fixed paraffin embedded human skin labeling S100 with ab4066.

This image was generated using the ascites version of the product.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066)

Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

This image was generated using the ascites version of the product.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066)

Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

This image was generated using the ascites version of the product.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4C4.9

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Full Length Protein corresponding to Cow Protein S100-A1. The exact immunogen used to generate this antibody is proprietary information.

P02639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/10 - 1/50", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 29/01/2021. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Product Specifications
Anti-S100 antibody [4C4.9] (ab4066) is a mouse monoclonal antibody and is validated for use in IHC-P in human samples.
Anti-S100 antibody [4C4.9] (ab4066) specifically detects S100 (UniProt ID: P23297; Molecular weight: 11kDa) and is sold in 500 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-S100 antibody [4C4.9] (ab4066) has high sensitivity and specificity.
Anti-S100 antibody [4C4.9] (ab4066) has been cited over 87 times in peer reviewed journals and is trusted by the scientific community.
Anti-S100 antibody [4C4.9] (ab4066) has 11 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone 4C4.9 - ab176243.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.3 - 7.5 Preservative: 0.05% Sodium azide Constituents: Tissue culture supernatant

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S100 protein family consists of low-molecular-weight proteins with individual members like S100B and S100A4 each having distinct roles. S100 proteins range typically between 9 to 13 kDa in mass. They are expressed broadly but especially in the central nervous system where glial cells are an important source and in several epithelial tissues including melanocytes. These proteins are noted for their calcium-binding properties facilitated by EF-hand motifs influencing both intracellular and extracellular processes.

Biological function summary

S100 proteins drive diverse cellular functions such as regulation of cell cycle differentiation and motility. They often operate as dimers and participate in the formation of protein complexes impacting cellular signaling networks. By binding calcium S100 proteins undergo conformational changes that allow interaction with target proteins modulating processes like cytoskeleton dynamics and neuronal survival.

Pathways

S100 proteins are significant in the regulation of the MAPK/ERK and NF-kB pathways. These pathways are critical for cell proliferation apoptosis and response to environmental stress. S100B in particular interacts with the receptor for advanced glycation end-products (RAGE) influencing downstream signaling cascades and amplifying the cellular response to inflammatory stimuli.

S100 proteins are implicated in the progression of conditions such as melanoma and Alzheimer's disease. In melanoma S100B levels serve as a biomarker reflecting disease progression potentially interacting with p53 to diminish its tumor suppressor function. In Alzheimer's disease S100B overexpression correlates with neuroinflammation and neuronal damage highlighting its association with disease through pathways involving amyloid-beta and tau proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).

See full target information S100A1

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Publications (102)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113237 PubMed40894878

2025

Biomarkers for early detection and monitoring of abnormal brain development in mild fetal growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuto Onoda,Yuma Kitase,Jacques-Olivier Coq,Kazuto Ueda,Shinobu Shimizu,Masahiro Tsuji,Masahiro Hayakawa,Yoshiaki Sato

ACS chemical neuroscience 16:2035-2047 PubMed40421586

2025

Reactive Astrocytes with Reduced Function of Glutamate Transporters in the Knock-in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ipsit Srivastava,Julen Goikolea,Tamer Ayberk Kaya,María Latorre-Leal,Francesca Eroli,Marta Pereira Iglesias,Laura Álvarez-Jiménez,Luis Enrique Arroyo-García,Makoto Shimozawa,Per Nilsson,André Fisahn,Maria Lindskog,Silvia Maioli,Raúl Loera-Valencia

Materials today. Bio 31:101588 PubMed40070866

2025

Multifunctional hydrogel loaded with 4-octyl itaconate and exosomes to induce bone regeneration for diabetic infected bone defect via Keap1-Nrf2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Wan,Qing Gao,Bing Ye,Wenzhe Sun,Kaifang Chen,Xiaodong Guo

Bioactive materials 40:378-395 PubMed38978801

2024

Chitosan/PLGA-based tissue engineered nerve grafts with SKP-SC-EVs enhance sciatic nerve regeneration in dogs through miR-30b-5p-mediated regulation of axon growth.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomei Yu,Mi Shen,Daiyue Chen,Yan Li,Qiang Zhou,Chunyan Deng,Xinyang Zhou,Qi Zhang,Qianru He,Hongkui Wang,Meng Cong,Haiyan Shi,Xiaosong Gu,Songlin Zhou,Fei Ding

British journal of pharmacology 181:4028-4049 PubMed38936407

2024

Histone deacetylase inhibition mitigates cognitive deficits and astrocyte dysfunction induced by amyloid-β (Aβ) oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Luan Pereira Diniz,Juliana Morgado,Ana Paula Bergamo Araujo,Leticia Maria da Silva Antônio,Hannah Paola Mota-Araujo,Pedro de Sena Murteira Pinheiro,Fernanda Savacini Sagrillo,Gabriele Vargas Cesar,Sérgio T Ferreira,Cláudia Pinto Figueiredo,Carlos Alberto Manssour Fraga,Flávia Carvalho Alcantara Gomes

Frontiers in oncology 14:1340184 PubMed38817895

2024

Analysis of tumor microenvironment composition in vestibular schwannomas: insights into NF2-associated and sporadic variations and their clinical correlations.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Nickl,David Ziebolz,Charlotte Rumpel,Dennis Klein,Robert Nickl,Eva Rampeltshammer,Camelia M Monoranu,Ralf-Ingo Ernestus,Cordula Matthies,Mario Löhr,Carsten Hagemann,Maria Breun

Cancer cell 42:1086-1105.e13 PubMed38788720

2024

Olfactory neuroblastoma mimics molecular heterogeneity and lineage trajectories of small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

John B Finlay,Abbie S Ireland,Sarah B Hawgood,Tony Reyes,Tiffany Ko,Rachelle R Olsen,Ralph Abi Hachem,David W Jang,Diana Bell,Joseph M Chan,Bradley J Goldstein,Trudy G Oliver

Brain research 1839:149020 PubMed38788929

2024

Morphological evidence for the potential protective effects of curcumin and Garcinia kola against diabetes in the rat hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Eda Denizci,Gamze Altun,Süleyman Kaplan

Biomaterials translational 4:180-195 PubMed38283091

2024

A comparative study of human and porcine-derived decellularised nerve matrices.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Li,Shuai Qiu,Weihong Yang,Zilong Rao,Jiaxin Chen,Yuexiong Yang,Qingtang Zhu,Xiaolin Liu,Ying Bai,Daping Quan

Journal of neuroinflammation 20:258 PubMed37946211

2023

M2 macrophage-derived cathepsin S promotes peripheral nerve regeneration via fibroblast-Schwann cell-signaling relay.

Applications

Unspecified application

Species

Unspecified reactive species

Eri Oshima,Yoshinori Hayashi,Zhen Xie,Hitoshi Sato,Suzuro Hitomi,Ikuko Shibuta,Kentaro Urata,Junjun Ni,Koichi Iwata,Tatsuo Shirota,Masamichi Shinoda

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 antibody [4C4.9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (102)

Product promise