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Anti-S100 antibody [4C4.9] is a mouse monoclonal antibody that is used to detect S100 in IHC-P. Suitable for Human samples.

- Cited in over 80 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (AB4066), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.3 - 7.5
Preservative: 0.05% Sodium azide
Constituents: Tissue culture supernatant

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Full Length Protein corresponding to Cow Protein S100-A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02639

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-P
Human
Tested

Tested
Tested

Species
Human
Dilution info
1/10.00000 - 1/50.00000
Notes

Antigen retrieval is not essential but may optimise staining.

Associated Products

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6 products for Alternative Product

Target data

Function

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed:12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed:23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed:22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed:12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed:11717446).

Alternative names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

Recommended products

Anti-S100 antibody [4C4.9] is a mouse monoclonal antibody that is used to detect S100 in IHC-P. Suitable for Human samples.

- Cited in over 80 publications

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Full Length Protein corresponding to Cow Protein S100-A1. The exact immunogen used to generate this antibody is proprietary information. Database link P02639
Clone number
4C4.9
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 29/01/2021. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Product Specifications
Anti-S100 antibody [4C4.9] (ab4066) is a mouse monoclonal antibody and is validated for use in IHC-P in human samples.
Anti-S100 antibody [4C4.9] (ab4066) specifically detects S100 (UniProt ID: P23297; Molecular weight: 11kDa) and is sold in 500 µL selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-S100 antibody [4C4.9] (ab4066) has high sensitivity and specificity.
Anti-S100 antibody [4C4.9] (ab4066) has been cited over 87 times in peer reviewed journals and is trusted by the scientific community.
Anti-S100 antibody [4C4.9] (ab4066) has 11 independent reviews from customers.

Related Products
Conjugation-ready, carrier free format available for antibody clone 4C4.9 - Anti-p53 antibody [PAb 240] - BSA and Azide free ab176243.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

    Immunohistochemistry of Formalin fixed paraffin embedded human skin labeling S100 with ab4066.
    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

    Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

    Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

    This image was generated using the ascites version of the product.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com