AB34686

Anti-S100 antibody

Name: Anti-S100 antibody. Rabbit polyclonal (ab34686) | Abcam
Brand: Abcam Limited
SKU: AB34686
Price: 445 USD
Availability: InStock
Rating: 5 (5 reviews)

(5 Reviews)

(13 Publications)

Rabbit Polyclonal S10A1 antibody. Suitable for WB, IHC-P and reacts with Cow, Rat, Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Native Full Length Protein corresponding to Cow Protein S100-A1.

View Alternative Names

S100A, S100A1, Protein S100-A1, S-100 protein alpha chain, S-100 protein subunit alpha, S100 calcium-binding protein A1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (AB34686)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (AB34686)

ab34686, at 1/500 dilution, staining S100 in formalin fixed and paraffin embedded human brain astrocyte tumor tissue by immunohistochemistry.

IHC-P

AbReview39444****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (AB34686)

ab34686 staining S100 in Rat nerve tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a 10mM citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/250 in PBS + 1x casein) for 1 hour at 25°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (AB34686)

ab34686, at a 1/500 dilution, staining S100 in mouse brain tissue by immunohistochemistry. The tissue was formalin fixed and embedded in paraffin. Dark nuclear staining is observed within basket cells located near the Purkinje cells in the cerebellum.

Unknown

Western blot - Anti-S100 antibody (AB34686)

All lanes:

Western blot - Anti-S100 antibody (ab34686) at 1/1000 dilution

Lane 1:

Bovine S100 monomer at 0.1 µg

Lane 2:

S100 from rat brain lysate at 35 µg

Secondary

All lanes:

IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX

Predicted band size: 10 kDa

Observed band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Cow, Human

Applications

IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Cow Protein S100-A1.

P02639

Specificity

A BLAST analysis was used to suggest reactivity with this protein from bovine based on 100% homology with the immunogen sequence. A 98% homology is noted for S100 alpha chain from primate sources. Mouse, rat and dog show 94% homology with the bovine S100 alpha sequence. Expect cross reactivity with S100 from most mammalian sources. Cross reactivity with S100 from other specific sources has not been determined. Homologies for the S100 beta chain are similar.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/2000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/2000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/200 - 1/2000", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mammals": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Please note that lot 296545 is supplied at 0.5mg/ml.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

This Protein A purified antibody is directed against bovine S100 protein. The product was purified from monospecific antiserum by Protein A chromatography.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S100 protein family consists of low-molecular-weight proteins with individual members like S100B and S100A4 each having distinct roles. S100 proteins range typically between 9 to 13 kDa in mass. They are expressed broadly but especially in the central nervous system where glial cells are an important source and in several epithelial tissues including melanocytes. These proteins are noted for their calcium-binding properties facilitated by EF-hand motifs influencing both intracellular and extracellular processes.

Biological function summary

S100 proteins drive diverse cellular functions such as regulation of cell cycle differentiation and motility. They often operate as dimers and participate in the formation of protein complexes impacting cellular signaling networks. By binding calcium S100 proteins undergo conformational changes that allow interaction with target proteins modulating processes like cytoskeleton dynamics and neuronal survival.

Pathways

S100 proteins are significant in the regulation of the MAPK/ERK and NF-kB pathways. These pathways are critical for cell proliferation apoptosis and response to environmental stress. S100B in particular interacts with the receptor for advanced glycation end-products (RAGE) influencing downstream signaling cascades and amplifying the cellular response to inflammatory stimuli.

S100 proteins are implicated in the progression of conditions such as melanoma and Alzheimer's disease. In melanoma S100B levels serve as a biomarker reflecting disease progression potentially interacting with p53 to diminish its tumor suppressor function. In Alzheimer's disease S100B overexpression correlates with neuroinflammation and neuronal damage highlighting its association with disease through pathways involving amyloid-beta and tau proteins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Small calcium binding protein that plays important roles in several biological processes such as Ca(2+) homeostasis, chondrocyte biology and cardiomyocyte regulation (PubMed : 12804600). In response to an increase in intracellular Ca(2+) levels, binds calcium which triggers conformational changes (PubMed : 23351007). These changes allow interactions with specific target proteins and modulate their activity (PubMed : 22399290). Regulates a network in cardiomyocytes controlling sarcoplasmic reticulum Ca(2+) cycling and mitochondrial function through interaction with the ryanodine receptors RYR1 and RYR2, sarcoplasmic reticulum Ca(2+)-ATPase/ATP2A2 and mitochondrial F1-ATPase (PubMed : 12804600). Facilitates diastolic Ca(2+) dissociation and myofilament mechanics in order to improve relaxation during diastole (PubMed : 11717446).

See full target information S100A1

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 15:87 PubMed40556005

2025

FRRS1L variants and ferriheme overload drive hyperpigmentation and systemic Iron overload in lanping black bone sheep.

Applications

Unspecified application

Species

Unspecified reactive species

Deping Han,Yuanyuan Zhang,Weidong Deng,Xue Yang,Jianfei Chen,Guoying Hua,Hesham Y A Darwish,Huaming Mao,Xiao Gou,Jiankui Wang,Kai Cui,Yuhao Ma,Yurong Tai,Xianggui Dong,Yanzhu Yao,Zu Yang,Suying Cao,Zhengquan Yu,Wansheng Liu,Xuemei Deng

Nature communications 16:2975 PubMed40140393

2025

Non-muscle myosin II inhibition at the site of axon injury increases axon regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Keunjung Heo,Tammy Szu-Yu Ho,Xiangsunze Zeng,Bruna Lenfers Turnes,Maryam Arab,Selwyn Jayakar,Kuchuan Chen,Georgios Kimourtzis,Michael C Condro,Elisa Fazzari,Xuan Song,J Tabitha Hees,Zhuqiu Xu,Xirui Chen,Lee B Barrett,Laura Perrault,Roshan Pandey,Kathleen Zhang,Aparna Bhaduri,Zhigang He,Harley I Kornblum,Jed Hubbs,Clifford J Woolf

Stem cell research & therapy 15:503 PubMed39736729

2024

A chitosan/acellular matrix-based neural graft carrying mesenchymal stem cells to promote peripheral nerve repair.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifa Zhang,Molin Li,Gang Cheng,Peng Wang,Chunhui Zhou,Yang Liu,Xiaofeng Duan,Jing Wang,Fang Xie,Yaqiong Zhu,Jianning Zhang

Frontiers in neuroscience 17:1103437 PubMed37250404

2023

Engineering circuits of human iPSC-derived neurons and rat primary glia.

Applications

Unspecified application

Species

Unspecified reactive species

Sophie Girardin,Stephan J Ihle,Arianna Menghini,Magdalena Krubner,Leonardo Tognola,Jens Duru,Isabelle Fruh,Matthias Müller,Tobias Ruff,János Vörös

Odontology 111:630-639 PubMed36460825

2022

Regeneration of sensory nerve branches in extraction socket and surrounding alveolar bone in rat: immunohistochemical observation of the axon and myelin sheath changes.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Kakuta,Satoru Matsunaga,Yuto Otsu,Kei Kitamura,Shinichi Abe,Yasutomo Yajima,Hideshi Sekine

Membranes 12: PubMed35877922

2022

Small Intestinal Submucosa Biomimetic Periosteum Promotes Bone Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Su,Bing Ye,Lian Zeng,Zekang Xiong,Tingfang Sun,Kaifang Chen,Qiuyue Ding,Weijie Su,Xirui Jing,Qing Gao,Guixiong Huang,Yizhou Wan,Xu Yang,Xiaodong Guo

Journal of anatomy 241:1211-1218 PubMed35728923

2022

Cultured dissociated primary dorsal root ganglion neurons from adult horses enable study of axonal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Adalbert,Stephen Cahalan,Eleanor L Hopkins,Abdulaziz Almuhanna,Andrea Loreto,Erzsébet Pór,Laura Körmöczy,Justin Perkins,Michael P Coleman,Richard J Piercy

Molecular therapy. Nucleic acids 28:342-352 PubMed35474735

2022

Establishment of a pancreatic cancer animal model using the pancreas-targeted hydrodynamic gene delivery method.

Applications

Unspecified application

Species

Unspecified reactive species

Osamu Shibata,Kenya Kamimura,Yuto Tanaka,Kohei Ogawa,Takashi Owaki,Chiyumi Oda,Shinichi Morita,Atsushi Kimura,Hiroyuki Abe,Satoshi Ikarashi,Kazunao Hayashi,Takeshi Yokoo,Shuji Terai

International journal of molecular sciences 22: PubMed34769029

2021

Involvement of the miR-363-5p/P2RX4 Axis in Regulating Schwann Cell Phenotype after Nerve Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Jung Sohn,Yun-Kyeong Nam,Hwan-Tae Park

Journal of neuroinflammation 18:172 PubMed34372877

2021

Increasing toll-like receptor 2 on astrocytes induced by Schwann cell-derived exosomes promotes recovery by inhibiting CSPGs deposition after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Dayu Pan,Yongjin Li,Fuhan Yang,Zenghui Lv,Shibo Zhu,Yixin Shao,Ying Huang,Guangzhi Ning,Shiqing Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (AB34686)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (AB34686)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody (AB34686)

Western blot - Anti-S100 antibody (AB34686)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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