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Rabbit Polyclonal S100 beta antibody. Astrocyte marker. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human S100B.


Images

Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914), expandable thumbnail
  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (AB227914), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (AB227914), expandable thumbnail
  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (AB227914), expandable thumbnail
  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (AB227914), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human S100B. Database link P04271

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-Fr
Human
Tested
Expected
Expected
Mouse
Tested
Tested
Tested
Rat
Tested
Expected
Expected
Cow
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Rhesus monkey
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/500 - 1/3000

Notes

-

Species

Rat

Dilution info

1/500 - 1/3000

Notes

-

Species

Human

Dilution info

1/500 - 1/3000

Notes

-

Predicted
Predicted

Species

Rabbit, Cow, Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow, Rhesus monkey

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Rat, Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rabbit, Cow, Rhesus monkey

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:20950652, PubMed:6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed:20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed:22399290).

Alternative names

Recommended products

Rabbit Polyclonal S100 beta antibody. Astrocyte marker. Suitable for WB, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human S100B.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human S100B. Database link P04271
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.

Biological function summary

The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.

Pathways

S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.

Associated diseases and disorders

S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.

Product promise

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6 product images

  • Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    Frozen sections of adult mouse retina tissue stained for S100 beta using ab227914 at 1/250 dilution in immunohistochemical analysis.

    Counter stain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/250 dilution (red).
    Nuclear counter stain: Fluoroshield with DAPI (blue).

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914), expandable thumbnail

    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    15% SDS-PAGE gel.

    All lanes: Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution

    Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

    Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with V5-human S100 beta, whole cell extract at 30 µg

    Predicted band size: 10 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    Paraffin-embedded mouse hind brain tissue stained for S100 beta using ab227914 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914), expandable thumbnail

    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    15% SDS-PAGE gel.

    All lanes: Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/500 dilution

    All lanes: A-375 (human malignant melanoma cell line) whole cell extract at 30 µg

    Predicted band size: 10 kDa

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914), expandable thumbnail

    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    15% SDS-PAGE gel.

    All lanes: Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution

    All lanes: Mouse brain tissue extract at 50 µg

    Predicted band size: 10 kDa

  • Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914), expandable thumbnail

    Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914)

    15% SDS-PAGE gel.

    All lanes: Western blot - Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution

    All lanes: Rat brain tissue extract at 50 µg

    Predicted band size: 10 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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