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Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker is a rabbit recombinant monoclonal antibody that is used to detect S100 beta in ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Validated on the Leica BOND™ RX automated IHC staining platform for S100 beta IHC
- Cited in over 390 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642), expandable thumbnail
  • Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (AB52642), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-PIHC-Fr
Human
Not recommended
Tested
Tested
Tested
Tested
Mouse
Not recommended
Tested
Expected
Tested
Tested
Rat
Not recommended
Tested
Not recommended
Tested
Tested
Goat
Not recommended
Predicted
Not recommended
Predicted
Predicted
Macaque monkey
Not recommended
Predicted
Not recommended
Predicted
Predicted
Zebrafish
Not recommended
Predicted
Not recommended
Predicted
Predicted

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Species
Goat
Dilution info
-
Notes

-

Species
Zebrafish
Dilution info
-
Notes

-

Species
Macaque monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/1000 - 1/5000
Notes

-

Species
Human
Dilution info
1/1000 - 1/5000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species
Goat, Zebrafish, Macaque monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Rat, Goat, Zebrafish, Macaque monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/200 - 1/1000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/200 - 1/1000
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Predicted
Predicted

Species
Goat, Zebrafish, Macaque monkey
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Human
Dilution info
-
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Species
Mouse
Dilution info
-
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Predicted
Predicted

Species
Goat, Zebrafish, Macaque monkey
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:20950652, PubMed:6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed:20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed:22399290).

Alternative names

Recommended products

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker is a rabbit recombinant monoclonal antibody that is used to detect S100 beta in ICC/IF, IHC-Fr, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Validated on the Leica BOND™ RX automated IHC staining platform for S100 beta IHC
- Cited in over 390 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP1576Y
Purification technique
Affinity purification Protein A
Dissociation constant
5.5 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-Fr IHC-P and WB.

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) was first used in a scientific publication in 2009 and has been cited over 399 times in peer reviewed journals. It's performance in IHC in mouse and human samples is trusted by the scientific community.

Abcam's high quality manufacturing and validation processes ensure Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) has 35 independent reviews from customers.

Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) specifically detects S100 beta (UniProt ID: P04271; Molecular weight: 11kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EP1576Y - Anti-S100 beta antibody [EP1576Y] - BSA and Azide free ab215989.

Antibody clone EP1576Y is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 647, Alexa Fluor® 594, Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 568 (HRP Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab195535, Alexa Fluor® 647 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab196175, Alexa Fluor® 594 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab307851, Alexa Fluor® 488 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab310990, Alexa Fluor® 555 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab313219, Alexa Fluor® 568 Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker ab313817).

S100B is a calcium-binding protein widely used in neuro research, particularly for its role as a biomarker in neurological diseases. It is expressed in glial cells and certain neurons and its levels can indicate brain injury and neuroinflammation. Researchers use S100B to assess glial activation and neuronal damage in conditions such as traumatic brain injury, stroke and neurodegenerative diseases (e.g. Alzheimer's disease). This makes S100B essential for understanding the extent of brain damage and the inflammatory response in various neurological disorders.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein S100 beta also known simply as S100B is a member of the S100 protein family which consists of low-molecular-weight proteins characterized by the presence of two EF-hand calcium-binding motifs. It has a mass of approximately 10.7 kDa. S100 beta is abundantly expressed in astrocytes oligodendrocytes and Schwann cells within the central nervous system and is also found in a variety of other tissues. Additionally the protein often serves as a beta marker in these cells and plays a role in regulating intracellular calcium levels and cell proliferation.

Biological function summary

The functions of S100 beta involve modulating a range of cellular processes such as the dynamics of cytoskeleton cell growth and differentiation. It does not act alone but often forms homodimers or heterodimers and engages with target proteins influencing their activity. The expression and release of S100 beta can affect neuron growth and survival which makes it notable as one of the astrocyte markers used in immunohistochemistry to study neural tissue and brain injury responses.

Pathways

Research has shown that S100 beta actively participates in the regulation of important signaling pathways including the ERK and NF-κB pathways. These pathways are involved in various cellular responses like inflammation and cell survival. In its signaling roles S100 beta functions in connection with proteins like the receptor for advanced glycation end-products (RAGE) helping to transmit extracellular signals into the cell.

Associated diseases and disorders

S100 beta has become associated with certain neurodegenerative diseases and cancer. Elevated levels of S100 beta have been observed in patients with Alzheimer's disease where the protein is believed to influence tau phosphorylation and amyloid-beta formation alongside interactions with proteins like tau. In the context of melanoma S100 beta serves as a marker indicating tumor progression and its interactions with proteins involved in cellular proliferation highlight its role in the disease process.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

18 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human cerebral cortex tissue using rabbit Anti-S100 beta antibody

    Immunohistochemical analysis of paraffin embedded human cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail
    Image from Chen M et al. PLoS One. 2014;9(2):e89548. Fig 3.; doi: 10.1371/journal.pone.0089548.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta is present in CNV lesions.

    A) S100 beta expression in a normal WT mouse retina. Strong immunoreactivity is present in the astrocytes (arrow). The position of the inner nuclear layer (INL) and outer nuclear layer (ONL) are indicted. Scale bar is 50 μm.

    B) S100 beta expression in WT mouse retinal at day 7 post-laser treatment. S100B was detected in the outer plexiform layer (arrowheads). Strong S100 beta expression was detected at the site of CNV. Scale bar is 50 μm.

  • Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunocytochemistry/ Immunofluorescence staining of rat neurons and glia using rabbit Anti-S100 beta antibody

    ab52642 staining S100 beta in primary rat neurons/glia DIV14 (prepared from E18 rat hippocampal brain area obtained from Transnetyx Tissue by BrainBits LLC cat.no. SDHEP). The cells were fixed with 100% methanol (5 min) permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab52642 at 5μg/ml and Alexa Fluor® 488 Anti-Tubulin antibody [YOL1/34] - Microtubule Marker ab195883 Rat monoclonal to alpha Tubulin (Alexa Fluor® 488) at 2μg/ml (shown in green). The secondary antibody (shown in red) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083 Alexa Fluor® 647 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunocytochemistry/ Immunofluorescence staining of A-375 (human malignant melanoma cell line) cells using rabbit Anti-S100 beta antibody

    Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 A-375 (human malignant melanoma cell line) cells labeling S100 beta with purified ab52642 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 secondary antibody at 1/500 dilution (green). The nuclear counter stain is DAPI (blue). The negative control is as follows;
    ab52642 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

  • Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) at 1/5000 dilution

    All lanes: A-375 (human malignant melanoma cell line) at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

  • Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    IHC image of S100 beta staining in a section of frozen normal human cerebral cortex performed on a Leica BONDTM system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with Anti-Synaptophysin antibody [SP11] - Synaptic Marker ab16659, 1/5000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail
    Image from Selvadurai HJ & Mason JO. PLoS One. 2011;6(8):e23012. Epub 2011 Aug 8. Fig 5.; doi:10.1371/journal.pone.0023012; August 8 2011 PLoS ONE 6(8): e23012.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse brain tissue using rabbit Anti-S100 beta antibody

    Immunohistochemical analysis of embryonic mouse brain tissue staining S100 beta with unpurified ab52642.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunohistochemistry (Frozen sections) staining of rat cerebrum tissue using rabbit Anti-S100 beta antibody

    Immunohistochemistry (Frozen sections) analysis of rat cerebrum tissue sections labeling S100 beta with purified ab52642 at 1/100 (9.9 μg/ml) dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunohistochemistry (Frozen sections) staining of mouse cerebrum tissue using rabbit Anti-S100 beta antibody

    Immunohistochemistry (Frozen sections) analysis of mouse cerebrum tissue sections labeling S100 beta with purified ab52642 at 1/100 (9.9 μg/ml) dilution. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. DAPI was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    Immunohistochemical analysis of paraffin embedded rat cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta antibody ab52642 was used with Tissue Clearing Kit Tissue Clearing Kit - hydrophobic ab243298 to penetrate, stain and clear a 1 mm coronal section of mouse brain. Blue: DAPI, Green: S100 beta.
    Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain thick tissue sections and get more data from each valuable tissue section.
    To use this antibody with tissue clearing, use Tissue Clearing Kit Tissue Clearing Kit - hydrophobic ab243298. For 1 mm brain sections, we recommend a starting dilution of 1:200, and also using Goat Anti-Rabbit IgG H&L AlexaFluor488 (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1:400.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of mouse cerebral cortex tissue using rabbit Anti-S100 beta antibody

    Immunohistochemical analysis of paraffin embedded mouse cerebral cortex tissue labeling S100 beta with purified ab52642 at 1/1000 dilution. Secondary antibody was Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Counter stain: Hematoxylin.

    Negative control: Using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail
    Image from Gerli MFM et al. PLoS One. 2018;13(1):e0191497 Fig 3.; doi: 10.1371/journal.pone.0191497.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human peripheral nerves using rabbit Anti-S100 beta antibody

    Immunofluorescent imaging of native and acellular human peripheral nerve sections stained for the axon protein Neurofilaments (NF200), the Schwann's cell marker S100β (ab52642) and for the extracellular matrix proteins Laminin and Collagen IV. Sections were counterstained with DAPI to confirm the removal of the cell nuclei upon decellularization (scale bar: 100 μm).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail
    This image is courtesy of a customer review submitted by Heiko Locher.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    Unpurified ab52642 staining S100 beta in human spiral ganglion tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at room temperature; antigen retrieval was by heat mediation in citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in PBS-T + 1% BSA) for 12 hours. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

  • Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) at 1/10000 dilution

    Lane 1: Mouse spinal cord at 20 µg

    Lane 2: Rat brain at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

  • Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    Blocking and Diluting buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642) at 1/5000 dilution

    All lanes: B16F0 (mouse melanoma cell line) at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 10 kDa

    Observed band size: 11 kDa

  • OI-RD Scanning - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    OI-RD Scanning - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [EP1576Y] - Astrocyte Marker (ab52642)

    S100 beta Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of human cerebrum using rabbit Anti-S100 beta antibody

    Immunohistochemical analysis of formalin fixed paraffin embedded human cerebrum labelling S100 beta with ab52642 at a concentration of 0.01 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab52642 anti-S100 beta antibody [EP1576Y] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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