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AB242388

Anti-S100 beta antibody [SP127] - BSA and Azide free

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(1 Publication)

Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting S100 beta in Flow Cytometry, IHC-P. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B

16 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human spinal cord tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human bladder tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Flow Cytometry (Intracellular) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling S100 beta with purified ab115803 at 1 : 200 dilution (0.79 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab130748).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human esophagus tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human kidney tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Formalin-fixed, paraffin-embedded human breast tissue stained for S100 beta using ab115803 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab115803).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 beta antibody [SP127] - BSA and Azide free (AB242388)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling S100 beta with ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115803)

  • Unconjugated

    Anti-S100 beta antibody [SP127] - Astrocyte Marker

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP127

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Mouse": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Rat": { "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Incubate with primary antibody for 10 minutes at room temperature.</p>" }, "Cow": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

What is this antibody validated in?
Anti-S100 beta antibody [SP127] - BSA and Azide free (ab242388) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

Collaboration
Anti-S100 beta [SP127] - BSA and Azide free (ab242388) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Other related products
We have a range of other formats of antibody clone [SP127] also available for your convenience: ab115803, Carrier free - ab242388, ab279621

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.
Biological function summary

The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.

Pathways

S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.

S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed : 20950652, PubMed : 6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed : 20950652, PubMed : 6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed : 20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed : 22399290).
See full target information S100B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cardiovascular medicine 11:1360364 PubMed38576426

2024

SARS-CoV-2 infection of endothelial cells, dependent on flow-induced ACE2 expression, drives hypercytokinemia in a vascularized microphysiological system.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher J Hatch,Sebastian D Piombo,Jennifer S Fang,Johannes S Gach,Makena L Ewald,William K Van Trigt,Brian G Coon,Jay M Tong,Donald N Forthal,Christopher C W Hughes
View all publications
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