Rabbit Recombinant Monoclonal S100 beta antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Flow Cyt (Intra) | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Expected | Tested |
Rat | Expected | Tested |
Cow | Predicted | Predicted |
Rabbit | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Incubate with primary antibody for 10 minutes at room temperature. |
Species Rat | Dilution info - | Notes Incubate with primary antibody for 10 minutes at room temperature. |
Species Human | Dilution info - | Notes Incubate with primary antibody for 10 minutes at room temperature. |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit, Cow | Dilution info - | Notes - |
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Small zinc- and- and calcium-binding protein that is highly expressed in astrocytes and constitutes one of the most abundant soluble proteins in brain (PubMed:20950652, PubMed:6487634). Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer (PubMed:20950652, PubMed:6487634). Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites (By similarity). Acts as a neurotrophic factor that promotes astrocytosis and axonal proliferation (By similarity). Involved in innervation of thermogenic adipose tissue by acting as an adipocyte-derived neurotrophic factor that promotes sympathetic innervation of adipose tissue (By similarity). Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase (By similarity). Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization (PubMed:20351179). May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity (PubMed:22399290).
Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100B
Rabbit Recombinant Monoclonal S100 beta antibody. Carrier free. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
SP127
Affinity purification Protein A/G
Purified from TCS by Protein A/G.
Blue Ice
+4°C
+4°C
Do Not Freeze
ab242388 is the carrier-free version of Anti-S100 beta antibody [SP127] ab115803.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
S100 beta also known as protein S100 is part of the S100 family of proteins characterized by their calcium-binding properties. The S100 beta protein has an approximate molecular mass of 10.7 kDa. This protein is mainly expressed in astrocytes a type of glial cell in the central nervous system. It acts as a marker for astrocytes and is prominently used in astrocyte immunohistochemistry. Additionally it serves as a valuable marker for identifying and studying astrocyte markers because it can highlight the presence of these cells in various contexts.
The S100 beta protein participates in several intracellular and extracellular activities influencing cell proliferation and differentiation. It forms dimers and sometimes assembles into larger complexes mediated by the binding of calcium ions which alters its functional state. Within the nervous system S100 beta modulates the dynamics between neurons and glial cells affecting processes such as cell survival and neurite outgrowth. This dual role in both supporting and inhibiting various cellular actions highlights its importance in maintaining neural homeostasis.
S100 beta engages in signaling pathways related to inflammation and stress responses. It works within the MAPK and NF-kB pathways playing roles in cellular responses to oxidative stress. These pathways involve interactions with other proteins like p53 which S100 beta can modulate to influence apoptosis. The regulatory properties of S100 beta in these pathways link it with broader networks affecting cell fate decisions under pathological and physiological conditions.
S100 beta is associated with neurodegenerative diseases and cancer. Elevated levels of S100 beta are often found in Alzheimer's disease where it interacts with proteins like amyloid-beta contributing to neuroinflammation and neurotoxicity. In cancer particularly malignant melanoma S100 beta levels serve as a biomarker for tumor progression. Its interactions with p53 and other regulatory proteins highlight its involvement in oncogenic processes emphasizing its potential as a target for therapeutic intervention in these diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling S100 beta with purified Anti-S100 beta antibody [SP127] ab115803 at 1:200 dilution (0.79 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-alpha 1 Fetoprotein antibody [SP154] ab130748).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat cerebrum tissue sections labeling S100 beta with Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S100 beta antibody [SP127] ab115803)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue sections labeling S100 beta with Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S100 beta antibody [SP127] ab115803)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast tissue sections labeling S100 beta with Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S100 beta antibody [SP127] ab115803)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling S100 beta with Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution (0.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S100 beta antibody [SP127] ab115803)
Formalin-fixed, paraffin-embedded human esophagus tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human spinal cord tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-S100 beta antibody [SP127] ab115803)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human breast tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human bladder tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human adrenal gland tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human kidney tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human melanoma tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Formalin-fixed, paraffin-embedded human colon tissue stained for S100 beta using Anti-S100 beta antibody [SP127] ab115803 at 1/100 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-S100 beta antibody [SP127] ab115803).
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com