Rabbit Recombinant Monoclonal S100A10 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 11 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (AB76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99757.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (AB76472)")
![Western blot - Anti-S100A10 antibody [EPR3317] (AB76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img283.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)")
![Western blot - Anti-S100A10 antibody [EPR3317] (AB76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img106008.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)")
![Western blot - Anti-S100A10 antibody [EPR3317] (AB76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img916.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)")
![Western blot - Anti-S100A10 antibody [EPR3317] (AB76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img60676.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)")
Publications
Filter by
- Folia neuropathologica 61:273-2902023Astragaloside IV inhibits experimental autoimmune encephalomyelitis by modulating the polarization of both microglia/macrophages and astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJingwen Yu,Bingtao Mu,Minfang Guo,Chunyun Liu,Tao Meng,Yuqing Yan,Lijuan Song,Jiezhong Yu,Gajendra Kumar,Cungen MaPubMed 37818688
- iScience 26:1074482023Lethal adulthood myelin breakdown by oligodendrocyte-specific Ddx54 knockout.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroaki Oizumi,Yuki Miyamoto,Chika Seiwa,Masahiro Yamamoto,Nozomu Yoshioka,Seiichi Iizuka,Tomohiro Torii,Katsuya Ohbuchi,Kazushige Mizoguchi,Junji Yamauchi,Hiroaki AsouPubMed 37720086
- Frontiers in immunology 13:9668812022CSE/HS ameliorates colitis in mice protection of enteric glial cells and inhibition of the RhoA/ROCK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Wang,Yanyu Ding,Wenjun JiangPubMed 36189321
- Nature communications 13:11342022Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Wan,Wusheng Zhu,Ying Zhao,Xiaohao Zhang,Ruidong Ye,Meng Zuo,Pengfei Xu,Zhenqian Huang,Chunni Zhang,Yi Xie,Xinfeng LiuPubMed 35241660
- Brain pathology (Zurich, Switzerland) 32:e130472022Proteomic analysis of human hippocampal subfields provides new insights into the pathogenesis of Alzheimer's disease and the role of glial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanpan Gao,Jiaqi Liu,Jiayu Wang,Yifan Liu,Ling-Hui Zeng,Wei Ge,Chao MaPubMed 35016256
- Frontiers in cell and developmental biology 9:6916482021Neuron-Glia Crosstalk Plays a Major Role in the Neurotoxic Effects of Ketamine Extracellular Vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDonald H Penning,Simona Cazacu,Aharon Brodie,Vesna Jevtovic-Todorovic,Steve N Kalkanis,Michael Lewis,Chaya BrodiePubMed 34604212
- Frontiers in genetics 12:6555692021KCNMB2-AS1 Promotes Bladder Cancer Progression Through Sponging miR-374a-3p to Upregulate S100A10.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhua Zhu,Yan Huang,Yong Zhang,Rongfu Huang,Chunmei HuangPubMed 34367236
- OncoTargets and therapy 13:10607-106192020LINC01436 Promotes the Progression of Gastric Cancer via Regulating miR-513a-5p/APE1 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing-Dian Lu et. al.PubMed 33116638
- Cell biochemistry and function 38:859-8692020LINC00174 is an oncogenic lncRNA of hepatocellular carcinoma and regulates miR-320/S100A10 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Tao Zhao,Bao-Jin Chi,Yao Sun,Nan-Nan Chi,Xue-Mei Zhang,Jia-Bin Sun,Ying Chen,Yong XiaPubMed 32128852
- Frontiers in physiology 10:72019Annexin II Light Chain p11 Interacts With ENaC to Increase Functional Activity at the Membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTanya T Cheung et. al.PubMed 30800070
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/100.00000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/5000.00000 - 1/50000.00000 | Notes - |
Species Human | Dilution info 1/5000.00000 - 1/50000.00000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Alternative names
ANX2LG, CAL1L, CLP11, S100A10, Protein S100-A10, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11
Recommended products
Rabbit Recombinant Monoclonal S100A10 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3317
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
S100A10 also known as p11 is a member of the S100 protein family. It has a molecular weight of about 11 kDa. This protein usually exists in the cytoplasm and nucleus of many cell types including epithelial cells and immune cells. S100A10 shows high expression in tissues like lungs kidneys and the intestinal tract. This protein functions mechanically as a calcium-binding protein even though it actually does not bind calcium directly promoting its interaction with other proteins.
- Biological function summary
S100A10 is involved in different cellular processes such as membrane trafficking and cellular signaling. It forms a complex with annexin A2 which is vital for the regulation of processes like endocytosis and exocytosis. This complex helps stabilize plasma membrane phospholipids promoting various transport and signaling mechanisms. S100A10 also plays a role in cellular adhesion and migration connecting with actin cytoskeleton.
- Pathways
S100A10 takes part in pathways linked to cellular migration and angiogenesis. It connects with pathways modulating actin dynamics commonly interacting with other proteins like annexin A2 and ARF6. Additionally S100A10 is linked to the plasminogen activation pathway where it facilitates the conversion of plasminogen to plasmin impacting tissue remodeling processes and endothelial cell function.
- Associated diseases and disorders
S100A10 has been linked to conditions such as cancer and inflammatory diseases. It has an association with annexin A2 in many cancer types suggesting its role in promoting tumor growth and invasion. Additionally S100A10 appears to play a part in depression by influencing serotonin signaling. Changes in S100A10 levels can disrupt cellular environments suggesting its significant link to the pathophysiology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99757.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)
Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Western blot - Anti-S100A10 antibody [EPR3317] (ab76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img283.jpg "Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)")
Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)
Blocking buffer: 5% NFDM/TBSTAll lanes: Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution
Lane 1: HaCaT (Human skin keratinocyte) whole cell lysate at 20 µg
Lane 2: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa, 40 kDa
Exposure time: 180s
![Western blot - Anti-S100A10 antibody [EPR3317] (ab76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img106008.jpg "Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)")
Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/50000 dilution
Lane 1: HACAT cell lysate at 20 µg
Lane 2: A431 cell lysate at 20 µg
Lane 3: HT-29 cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa
![Western blot - Anti-S100A10 antibody [EPR3317] (ab76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img916.jpg "Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)")
Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)
Blocking buffer: 5% NFDM/TBSTLane 1: Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution
Lane 2: Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution
All lanes: Western blot - Recombinant Human S100A10 protein (His tag) (Recombinant Human S100A10 protein (His tag) ab221399) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa, 25 kDa
Exposure time: 3s
![Western blot - Anti-S100A10 antibody [EPR3317] (ab76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--western-blot-img60676.jpg "Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)")
Western blot - Anti-S100A10 antibody [EPR3317] (ab76472)
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution
Lane 1: RAW264.7 cell lysate at 20 µg
Lane 2: NIH/3T3 cell lysate at 20 µg
SecondaryAll lanes: HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 11 kDa
Observed band size: 12 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137657.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)
Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-ab76472--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67113.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (ab76472)
Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com