Anti-S100A10 antibody [EPR3317]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (AB76472)

Unpurified ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (AB76472)

Unpurified ab76472 at 1/100 dilution staining S100A10 in human colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] (AB76472)

Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• WB

Lab

Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

Negative control : Raji, SH-SY5Y, Neuro-2a.

We are unsure how to define the 50kDa band.

This antibody cannot detect BOI in tissue samples.

All lanes:

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution

Lane 1:

AsPC-1 (human Pancreas epithelial cell) whole cell lysate at 20 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate whole cell lysate at 20 µg

Lane 3:

HaCaT (Human skin keratinocyte) whole cell lysate at 20 µg

Lane 4:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

CT26.WT (mouse colon fibroblast) whole cell lysate at 20 µg

Lane 6:

Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

Mouse hippocampus tissue lysate at 20 µg

Lane 8:

Mouse cerebellum tissue lysate at 20 µg

Lane 9:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 12 kDa

false

Exposure time: 20s

• WB

Lab

Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/50000 dilution

Lane 1:

HACAT cell lysate at 20 µg

Lane 2:

A431 cell lysate at 20 µg

Lane 3:

HT-29 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 12 kDa

false

• WB

Lab

Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)

Blocking buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/10000 dilution

Lane 1:

HaCaT (Human skin keratinocyte) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa,40 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602).

S100A10 shows lower enrichment in tissue samples than in cell lines. No signal was observed on brain samples. This product is not suitable for use on brain-related samples (such as astrocytes, hippocampus, and cerebellum) due to its low expression level in those tissues.

It is recommended to optimize experimental conditions by using 14% SDS-PAGE gel, increasing the sample loading amount, using a lower antibody dilution ratio, and employing femtogram-level sensitivity substrates.

All lanes:

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution

Lane 1:

HaCaT (Human skin keratinocyte) whole cell lysate at 20 µg

Lane 2:

Human brain tissue lysate at 20 µg

Lane 3:

Human lung tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse hippocampus tissue at 20 µg

Lane 6:

Mouse lung tissue lysate at 20 µg

Lane 7:

Rat brain tissue lysate at 20 µg

Lane 8:

Rat hippocampus tissue lysate at 20 µg

Lane 9:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 11 kDa

false

Exposure time: 100s

• WB

Lab

Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)

Blocking buffer : 5% NFDM/TBST

Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution

Lane 1:

RAW264.7 cell lysate at 20 µg

Lane 2:

NIH/3T3 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit (H+L) at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 12 kDa

false

• WB

Lab

Western blot - Anti-S100A10 antibody [EPR3317] (AB76472)

Blocking buffer : 5% NFDM/TBST

Lane 1:

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/1000 dilution

Lane 2:

Western blot - Anti-S100A10 antibody [EPR3317] (ab76472) at 1/5000 dilution

All lanes:

Western blot - Recombinant Human S100A10 protein (His tag) (<a href='/en-us/products/proteins-peptides/recombinant-human-s100a10-protein-his-tag-ab221399'>ab221399</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

Observed band size: 13 kDa,25 kDa

false

Exposure time: 3s