Rabbit Recombinant Monoclonal S100A10 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99757.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524)")
![Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--western-blot-img283.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524)")
![Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--western-blot-img916.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138304.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67112.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (AB232524)")
Publications
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- Journal of neuroinflammation 20:442023The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCong Wang,Longxuan LiPubMed 36823628
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected |
| Mouse | Expected | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Alternative names
ANX2LG, CAL1L, CLP11, S100A10, Protein S100-A10, Calpactin I light chain, Calpactin-1 light chain, Cellular ligand of annexin II, S100 calcium-binding protein A10, p10 protein, p11
Recommended products
Rabbit Recombinant Monoclonal S100A10 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR3317
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse samples.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232524 is the carrier-free version of Anti-S100A10 antibody [EPR3317] ab76472.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
S100A10 also known as p11 is a member of the S100 protein family. It has a molecular weight of about 11 kDa. This protein usually exists in the cytoplasm and nucleus of many cell types including epithelial cells and immune cells. S100A10 shows high expression in tissues like lungs kidneys and the intestinal tract. This protein functions mechanically as a calcium-binding protein even though it actually does not bind calcium directly promoting its interaction with other proteins.
- Biological function summary
S100A10 is involved in different cellular processes such as membrane trafficking and cellular signaling. It forms a complex with annexin A2 which is vital for the regulation of processes like endocytosis and exocytosis. This complex helps stabilize plasma membrane phospholipids promoting various transport and signaling mechanisms. S100A10 also plays a role in cellular adhesion and migration connecting with actin cytoskeleton.
- Pathways
S100A10 takes part in pathways linked to cellular migration and angiogenesis. It connects with pathways modulating actin dynamics commonly interacting with other proteins like annexin A2 and ARF6. Additionally S100A10 is linked to the plasminogen activation pathway where it facilitates the conversion of plasminogen to plasmin impacting tissue remodeling processes and endothelial cell function.
- Associated diseases and disorders
S100A10 has been linked to conditions such as cancer and inflammatory diseases. It has an association with annexin A2 in many cancer types suggesting its role in promoting tumor growth and invasion. Additionally S100A10 appears to play a part in depression by influencing serotonin signaling. Changes in S100A10 levels can disrupt cellular environments suggesting its significant link to the pathophysiology of these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img99757.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
This data was developed using Anti-S100A10 antibody [EPR3317] ab76472, the same antibody clone in a different buffer formulation.
Immunohistochemical staining of paraffin embedded human squamous cell cervical carcinoma with purified Anti-S100A10 antibody [EPR3317] ab76472 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
![Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--western-blot-img283.jpg "Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)")
Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
Blocking buffer: 5% NFDM/TBSTThis data was developed using Anti-S100A10 antibody [EPR3317] ab76472, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-S100A10 antibody [EPR3317] (Anti-S100A10 antibody [EPR3317] ab76472) at 1/10000 dilution
Lane 1: HaCaT (Human skin keratinocyte) whole cell lysate at 20 µg
Lane 2: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 3: SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa, 40 kDa
Exposure time: 180s
![Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--western-blot-img916.jpg "Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)")
Western blot - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
Blocking buffer: 5% NFDM/TBSTThis data was developed using Anti-S100A10 antibody [EPR3317] ab76472, the same antibody clone in a different buffer formulation.
Lane 1: Western blot - Anti-S100A10 antibody [EPR3317] (Anti-S100A10 antibody [EPR3317] ab76472) at 1/1000 dilution
Lane 2: Western blot - Anti-S100A10 antibody [EPR3317] (Anti-S100A10 antibody [EPR3317] ab76472) at 1/5000 dilution
All lanes: Western blot - Recombinant Human S100A10 protein (His tag) (Recombinant Human S100A10 protein (His tag) ab221399) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 13 kDa, 25 kDa
Exposure time: 3s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img138304.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
This data was developed using Anti-S100A10 antibody [EPR3317] ab76472, the same antibody clone in a different buffer formulation.
Unpurified Anti-S100A10 antibody [EPR3317] ab76472 at 1/100 dilution staining S100A10 in human colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524), expandable thumbnail](https://content.abcam.com/products/images/s100a10-antibody-epr3317-bsa-and-azide-free-ab232524--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67112.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A10 antibody [EPR3317] - BSA and Azide free (ab232524)
This data was developed using Anti-S100A10 antibody [EPR3317] ab76472, the same antibody clone in a different buffer formulation.
Unpurified Anti-S100A10 antibody [EPR3317] ab76472 at 1/100 dilution staining S100A10 in human kidney tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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