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AB169530

Anti-S100A11 antibody [EPR11171(B)]

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(5 Publications)

Rabbit Recombinant Monoclonal S100A11 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

MLN70, S100C, S100A11, Protein S100-A11, Calgizzarin, Metastatic lymph node gene 70 protein, Protein S100-C, S100 calcium-binding protein A11, MLN 70

3 Images
Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11171(B)] (AB169530)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11171(B)] (AB169530)

Intracellular flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (AB169530)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] (AB169530)

Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).

Western blot - Anti-S100A11 antibody [EPR11171(B)] (AB169530)
  • WB

Unknown

Western blot - Anti-S100A11 antibody [EPR11171(B)] (AB169530)

All lanes:

Western blot - Anti-S100A11 antibody [EPR11171(B)] (ab169530) at 1/1000 dilution

Lane 1:

SKBR3 cell lysate at 10 µg

Lane 2:

BxPC-3 cell lysate at 10 µg

Lane 3:

PC-3 cell lysate at 10 µg

Lane 4:

HACAT cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11 kDa

false

  • Carrier free

    Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11171(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A11 also known as Calgizzarin or MLN 70 is a calcium-binding protein of the S100 family. It has a molecular mass of approximately 11 kDa. S100A11 can be found in a wide range of tissues including skin placenta and heart. It exhibits the ability to bind calcium ions through its EF-hand motifs which influences its activity and stability. These calcium-binding domains make S100A11 an important regulator of various intracellular processes by responding to changes in calcium ion concentrations.
Biological function summary

The functions of S100A11 include involvement in cell proliferation differentiation and migration. S100A11 often operates as part of a protein complex facilitating these cellular processes by interacting with other signaling molecules. It can shuttle between the cytoplasm and nucleus influencing gene expression and cellular dynamics. S100A11 has also shown involvement in smooth muscle contraction and skin barrier function regulation.

Pathways

The activity involving S100A11 spans multiple biological pathways including the calcium signaling pathway and the epithelial cell signaling pathway. Through these pathways S100A11 maintains critical cellular homeostasis and function. In the calcium signaling pathway it interacts with proteins like annexins and calcyclin to modulate cellular responses to calcium influx. In epithelial cell signaling it affects pathways related to cell adhesion and communication integrating signals that dictate cell behavior in response to external stimuli.

S100A11 is relevant to cancer progression and inflammatory skin disorders. Its overexpression in various cancers such as bladder and prostate cancer suggests a role in tumor growth and metastasis. During the cancerous process S100A11 interacts with the p53 protein which impacts cell cycle regulation and survival. Additionally in inflammatory skin conditions like psoriasis S100A11 influences keratinocyte activity contributing to abnormal skin scaling and inflammation through interactions with filaggrin and keratin proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Facilitates the differentiation and the cornification of keratinocytes.
See full target information S100A11
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 11:e16128 PubMed37927791

2023

Mechanism of LEF1-AS1 regulating HUVEC cells by targeting miR-489-3p/S100A11 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Haoran Zhang,Wenqiu Wang,Junjie Lin,Junbo Qiao,Xinjun Wang,Bin Fang,Changkuan Chen,Yujiao Wang,Gaozan Zhu,Wenbo Liu

Frontiers in immunology 13:911997 PubMed35799787

2022

Proteomic Analyses Reveal Higher Levels of Neutrophil Activation in Men Than in Women With Systemic Lupus Erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Long Cai,Lan Gui,He Huang,Yu-Kun Zhang,Li Zhang,Zhu Chen,Yu-Jun Sheng

American journal of cancer research 12:1934-1959 PubMed35693076

2022

Circ_0006790 carried by bone marrow mesenchymal stem cell-derived exosomes regulates S100A11 DNA methylation through binding to CBX7 in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Gao,Liqiang Wang,Changfeng Li

Bioengineered 12:9081-9093 PubMed34654357

2021

Circular RNA FOXP1 relieves trophoblastic cell dysfunction in recurrent pregnancy loss via the miR-143-3p/S100A11 cascade.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gao,Yukun Tang,Qian Sun,Guixue Guan,Xiaoyan Wu,Fan Shi,Zihao Zhou,Wen Yang

Cell biochemistry and function 37:72-83 PubMed30773657

2019

Proteomic analysis of the effects of cell culture density on the metastasis of breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Man-Lan Guo,Mi-Xin Sun,Jin-Zhi Lan,Li-Sha Yan,Jing-Juan Zhang,Xiao-Xia Hu,Shu Xu,Da-Hua Mao,Hai-Song Yang,Ya-Wei Liu,Teng-Xiang Chen
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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