Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)

This data was developed using ab169530, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)

This data was developed using ab169530, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).

• WB

Lab

Western blot - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)

This data was developed using ab169530, the same antibody clone in a different buffer formulation.

Western blot : Anti-S100A11 antibody [EPR11171(B)] ab169530 staining at 1/1000 dilution; Mouse anti GAPDH ab8245 loading control staining at 1/20000 dilution, shown in magenta. A band was observed at 12 kDa in Wild-type HeLa cell lysates with no signal observed at this size in S100A11 knockout HeLa cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween 20 (TBS-T) before incubation with primary antibodies overnight at 4 C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit HRP (H+L) and Goat anti-Mouse 680RD at 1/20,000 dilution. This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-S100A11 antibody [EPR11171(B)] (<a href='/en-us/products/primary-antibodies/s100a11-antibody-epr11171b-ab169530'>ab169530</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa at 20 µg

Lane 2:

Western blot - Human S100A11 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-s100a11-knockout-hela-cell-line-ab265085'>ab265085</a>) at 20 µg

Lane 3:

U-2 OS at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit HRP (H+L) & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

true

Exposure time: 10s

• WB

Unknown

Western blot - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)

This data was developed using ab169530, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-S100A11 antibody [EPR11171(B)] (<a href='/en-us/products/primary-antibodies/s100a11-antibody-epr11171b-ab169530'>ab169530</a>) at 1/1000 dilution

Lane 1:

SKBR3 cell lysate at 10 µg

Lane 2:

BxPC-3 cell lysate at 10 µg

Lane 3:

PC-3 cell lysate at 10 µg

Lane 4:

HACAT cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 11 kDa

false