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AB249483

Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal S100A11 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

MLN70, S100C, S100A11, Protein S100-A11, Calgizzarin, Metastatic lymph node gene 70 protein, Protein S100-C, S100 calcium-binding protein A11, MLN 70

3 Images
Western blot - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)
  • WB

Unknown

Western blot - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)

This data was developed using ab169530, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-S100A11 antibody [EPR11171(B)] (<a href='/en-us/products/primary-antibodies/s100a11-antibody-epr11171b-ab169530'>ab169530</a>) at 1/1000 dilution

Lane 1:

SKBR3 cell lysate at 10 µg

Lane 2:

BxPC-3 cell lysate at 10 µg

Lane 3:

PC-3 cell lysate at 10 µg

Lane 4:

HACAT cell lysate at 10 µg

Secondary

All lanes:

HRP labeled goat anti-rabbit at 1/2000 dilution

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Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)

This data was developed using ab169530, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of BxPC-3 cells labeling S100A11 with ab169530 at 1/250 dilution. DAPI nuclear staining (blue).

Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-S100A11 antibody [EPR11171(B)] - BSA and Azide free (AB249483)

This data was developed using ab169530, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HACAT cells labeling S100A11 with ab169530 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11171(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249483 is the carrier-free version of ab169530.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

S100A11 also known as Calgizzarin or MLN 70 is a calcium-binding protein of the S100 family. It has a molecular mass of approximately 11 kDa. S100A11 can be found in a wide range of tissues including skin placenta and heart. It exhibits the ability to bind calcium ions through its EF-hand motifs which influences its activity and stability. These calcium-binding domains make S100A11 an important regulator of various intracellular processes by responding to changes in calcium ion concentrations.
Biological function summary

The functions of S100A11 include involvement in cell proliferation differentiation and migration. S100A11 often operates as part of a protein complex facilitating these cellular processes by interacting with other signaling molecules. It can shuttle between the cytoplasm and nucleus influencing gene expression and cellular dynamics. S100A11 has also shown involvement in smooth muscle contraction and skin barrier function regulation.

Pathways

The activity involving S100A11 spans multiple biological pathways including the calcium signaling pathway and the epithelial cell signaling pathway. Through these pathways S100A11 maintains critical cellular homeostasis and function. In the calcium signaling pathway it interacts with proteins like annexins and calcyclin to modulate cellular responses to calcium influx. In epithelial cell signaling it affects pathways related to cell adhesion and communication integrating signals that dictate cell behavior in response to external stimuli.

S100A11 is relevant to cancer progression and inflammatory skin disorders. Its overexpression in various cancers such as bladder and prostate cancer suggests a role in tumor growth and metastasis. During the cancerous process S100A11 interacts with the p53 protein which impacts cell cycle regulation and survival. Additionally in inflammatory skin conditions like psoriasis S100A11 influences keratinocyte activity contributing to abnormal skin scaling and inflammation through interactions with filaggrin and keratin proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Facilitates the differentiation and the cornification of keratinocytes.
See full target information S100A11
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com