AB281212

Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture)

RabMAb
Recombinant
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Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture) (ab281212) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting S100A12/CGRP in ELISA. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Protein S100-A12, CGRP, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, Extracellular newly identified RAGE-binding protein, Migration inhibitory factor-related protein 6, Neutrophil S100 protein, S100 calcium-binding protein A12, CAAF1, CAGC, EN-RAGE, MRP-6, p6, S100A12

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Sandwich ELISA - Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture) (AB281212)

sELISA

Supplier Data

Sandwich ELISA - Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture) (AB281212)

Example of Human S100A12/CGRP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23677-121

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture) (ab281212) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human samples.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The S100A12 protein also known as Calgranulin C or CGRP (Calcitonin Gene-Related Peptide) has a molecular mass of approximately 10.4 kDa. This protein shows expression primarily in neutrophils but is also present in other types of cells such as epithelial cells and certain cell lines. S100A12 belongs to the S100 protein family which is part of the EF-hand calcium-binding proteins known for playing roles in cell regulation. The protein binds calcium ions which influence its structural conformation.

Biological function summary

S100A12 is important in the inflammatory response and immune system regulation. It acts as a pro-inflammatory mediator attracting leukocytes to sites of inflammation. Furthermore it can bind to receptors on cell surfaces like RAGE (Receptor for Advanced Glycation End Products) which induces signaling cascades that amplify immune responses. S100A12 does not function alone but often forms homodimers or heterodimers with other S100 proteins enhancing its biological activities.

Pathways

S100A12 participates in pathways like the inflammatory response pathway and the RAGE signaling pathway. By interacting with RAGE and possibly other receptors it impacts cellular responses associated with stress injury and microbial infection. This interaction connects S100A12 to other proteins in the same pathways such as NF-kB an important transcription factor involved in inflammation and immune responses.

S100A12 has connections with conditions like rheumatoid arthritis and inflammatory bowel disease. Elevated levels of S100A12 often correlate with disease activity and severity suggesting its potential as a biomarker. In rheumatoid arthritis it contributes to the inflammatory environment while in inflammatory bowel disease it associates not only with inflammation but also with proteins like IL-6 and TNF-alpha which are significant cytokines in the pathogenesis of these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its pro-inflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of pro-inflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus.

See full target information S100A12

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-S100A12/CGRP antibody [EPR23677-121] - BSA and Azide free (Capture) (AB281212)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise